FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002876079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5023
Sequences flagged as poor quality0
Sequence length71
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140327.93151503085805No Hit
GGAGTGAAAAGAAAGTGGGAATTCAGGAACTTGGACCACGTTTTACCTTAAAATTAAGGTCTCTTCAGAAA360.7167031654389807No Hit
CAGTTTTGGCTTCTTAAACTTCATATTTGGGTAGGTTAAGCTGCCATACGTGTTCAGTGTGAATAGTGTTT350.6967947441767868No Hit
GCTACTCAGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCC350.6967947441767868No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG280.5574357953414294No Hit
GATGATAAAGTTGGAACAGATATGCTGGAGGAGCAGATCACTGCTTTTGAGGACTATGTGCAGTCCATGGA280.5574357953414294No Hit
GTACCAAAAATTTACTTCTACTATCACACTAAAAAACATAACCAAAAATGGAGGCAGACCTCTGGCCATGA260.5176189528170416No Hit
GGGTGAGCACAGACAGGATCGCAGGGAGAGGCCGTATTAATTAGCCTGGCTCCCCAGGTTCTGGAACAGCT240.47780211029265385No Hit
CCAAGTACAGATGGACTGAGTACGGCCTGACGTTTACAGAGAAATGGAATACCGACAATACACTAGGCACC230.45789368903045985No Hit
AGACCAGCCTGGCCAACATGATGAAATCCCGTCTCTACTAAAAACACAAAAATTAGCTGGGCATGATTGCA220.437985267768266No Hit
GTATGTTAATGACTTGTTGGGTAGCTATTAAGGTACTAGAGTTGATAAATGTGTACAGGGTCCTTTTGCAA210.418076846506072No Hit
TTTTACAATCATTTCTGAATACAGAAGTTGTGGCCAAGTACAAATTATGGTATCTATTACTTTTTAAATGG210.418076846506072No Hit
GGACCTAAAAGACCTGTGACTAGTGAGCTCTAGGCTGTAGAAATTTAAAAACTACAATGTGATTAACTCGA210.418076846506072No Hit
ACATGTGTTACATAGGCAATAGTATTATCTCAATTCTATAAATGAGGAACTATCATTGTAAATGGAACATG210.418076846506072No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA210.418076846506072No Hit
GTACTGTTTCAAATCAAACCTCAGGGATTTAGAGATACTGTTGTTACTTCTGCATGTTCATTGCAAACAGA210.418076846506072No Hit
GCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAG200.3981684252438782No Hit
ACCTGATCCAAAAGCCAGTCTCAGGAGTTTACCCCTGGGATGGGGGATGCATCTGCACCTGACTTTGGGGC200.3981684252438782No Hit
GGGCGTGATGGTGCACACCTGTAGTCCCAGCTACTTGGAAGGCTGTGGCAGGAGAATGGCTTGAACAAGGG200.3981684252438782No Hit
GTTTTCACAGTAGAAGTTGCATCTTATTTAATGACTCTGATACATTGCAAGCACTCAGTAAATATCAGCAA200.3981684252438782No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.37826000398168425No Hit
ATGACTGATGATCCTCCAACAACAAAACCACTTACTGCTCGTAAATTCATTTGGACGAACCATAAATTCAA190.37826000398168425No Hit
CCTGGGTTCCCTAGGCCCTAGTGAGGGCAGAAGAGAAACCATCCTCTCCCTTCTTACACCGTGAGGCCAAG190.37826000398168425No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA180.35835158271949036No Hit
GTCTCGGCGGGAGGAGAAAAAAAGAGCAGAGAAGGAGCGAATCATGAATGAGGAAGATCCTGAGAAACAGC180.35835158271949036No Hit
AGTTAAACCATCCACTCCCTCACCTTCAGCCTTCAGTGAATGTGCTTTCTAGTTGTCAGGAATGCTGAAGA180.35835158271949036No Hit
GACAATGTTTTATTTGCCACCAGGCAGAACAGAATGAGCATTGGTAGAGAAGGCTCCCTCTTGGTATGTTA180.35835158271949036No Hit
GCTCTGCCTCCTGTGCTTATATCATCCAAAAACTTTTTAAAAAGGTCCAGAATTCTATTTTAACCTGATGT170.3384431614572964No Hit
CATAAGTTCAGTAGTTGCTTCCCTAACTGCAAAGGCAATCTCATTTAGTTGAGTAGCTCTTGAAAGCAGCT170.3384431614572964No Hit
CCTGGGGTTGTCCTGGCTCTGGGGAGAGAGACGGAGCCTTTAGTACAGCTATCTGCTGGCTCTAAACCTTC170.3384431614572964No Hit
GGGTACAAGCAGACTCTGAAGATGATCAGACAAGGCAAAGCGAAATTGGTCATTCTCGCTAACAACTGCCC170.3384431614572964No Hit
GTAAAGAAAATCATACAACTCAGCATCCAGTTGGCTTTTTAAGAATTCTGTACTTCCAATTTATAATGAAT160.31853474019510253No Hit
GTTTCAATGAGGTGCTGAAACAGTGCCGACTTGCAAACGGATTGGCCTAATGAAGAAGTTCAACCTGGAGA160.31853474019510253No Hit
GGATGTTTATAAACAAGACTAAGTCTGACTTCATAAGGGCTTTCTAAAACCATTTCTGTCCAAGAGAAAAT150.2986263189329086No Hit
GATCTGACTTCAGTTCTAGTAAGAGGGAAGCGGCCCTGATGAGGGCGTCCCTCTTTTGACTCTGCCCGTTT150.2986263189329086No Hit
CTGCCCCTCCCGCTGCGTGTCCAGGTCCAAAGTGGAGAGCCTGCCTTTGGGTTTAGTTCCCGAATCAGAAT150.2986263189329086No Hit
GTTCCAGTATTGGTTATGAGTCTTCTGTTCATCGCTTCTGTATTTATGTTGCACATTTGGGGCAAGTACAC140.2787178976707147No Hit
GGCCAGCCTAGGGGCCAGGCACATCGGGCACCTCCTCCCCATGGACTATAGCGCCAATGCCATTGCCTTCT140.2787178976707147No Hit
GCTGAAGTGTTACCAACTAGCCACACCATGAATTGTCCGTAATGTTCATTAACAGCATCTTTAAAACTGTG140.2787178976707147No Hit
GGACCAGCCTGGGCAATATAGTGAGACCTTGTCTCTACAAAAAATACAAAAAATTAACTGGGCGTAGTGGT140.2787178976707147No Hit
GCACCAGTGTCCACCCTATGAGAAGTCCCACCCAGGCAGCTGGGCGCAGTGGCTCACACCTGTAATCCCAG140.2787178976707147No Hit
GCATATGCCAAAAAATTTTAAGCAAATGAAAGCTACCAATTTAAAGTTACGGAATCTACCATTTTAAAGTT140.2787178976707147No Hit
TTCTTACATGTGTGTCTTGTTTTGTTTTTTCCAAATGTTCCACAATGGAGATTCATTACTTTTATGATATG130.2588094764085208No Hit
CACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCT130.2588094764085208No Hit
ACTCAGGAGGTTAAGTCAGGAGAATCACTTGAACCCAGGAGGCAGTGGTTGCAGTGAGCCGAGATCACACC130.2588094764085208No Hit
GCCACTGAGTATTTTTTTTGCCTTAGCCAGTGTACCTCCTACCTCAGTCTATGTGAGAGGAAGAGAATGTG130.2588094764085208No Hit
CCCTTACCATGGATAGCAAAAAGAGAATTGGCTGGCGATTCATAAGGATAGACACTTCTTGTGTATGTACA130.2588094764085208No Hit
GAACAGTGGGAAGAAAGAATACAGAACTGGCATGAAGAACATAGAGGAATGTTAAGGGAGGATTCTATGAT120.23890105514632692No Hit
GGTAGTAACAGCCCAATCTTGGGCTGTTAAATCCTAGGAAATCTCGAATCATAGTGATTAAAATAGTTGGG120.23890105514632692No Hit
GGTCTGGGAGGGGCTGAACGTGGTGAAGACAGGCCGAGTGATGCTTGGGGAGACCAATCCAGCAGATTCAA120.23890105514632692No Hit
GTTGATGGCCGCCATCCTCATGACATCATCGATGACATCAACAGTGGTGCTGTGGAATGCCCAGCCAGTTA120.23890105514632692No Hit
CTTTCTGCCCATGCTGCAGACAGTGGCCAAGAACAAGGACCAGGGCACCTATGAGGATTATGTCGAAGGAC120.23890105514632692No Hit
GGAATAACTTACATAACTTGGAATACTTAATGCATCATCCTTTCAAGTGCTTTTAGAATAAGATGTTGATT120.23890105514632692No Hit
GGGTGGTGCTAAGCGCCATCGTAAGGTGCTCCGGGATAACATCCAGGGCATTACAAAACCGGCTATTCGCC120.23890105514632692No Hit
GTCTGCTTTGACTGTACAATTTGTTCAAGGAATTTTTGTAGAAAAATACGATCCTACGATAGAAGATTCTT120.23890105514632692No Hit
GCCACAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAGAGCCCTTGCCCGCTC120.23890105514632692No Hit
GCACAAGAACCCTGAAGATCCTACTGAGGTGCCTGGTGGATTTTTAAGTGACCTGAACCTGGCATCACTAC120.23890105514632692No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT110.218992633884133No Hit
GTAAAGGAAAAGTATGATACAGATTTTTATATTCTTGATAAATATCCATTGGCTGTAAGACCTTTCTATAC110.218992633884133No Hit
GTTTAAAAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.218992633884133No Hit
ATTACATATGCATGCATAGGGGTACATTTACATTCTGTAAGAGATTGAGCCTGAACTCTCTTAGTCATAAA110.218992633884133No Hit
CCGGAGGCCTGACTCTGGGAGCACGCACGCACAACTACGGGATTGGCGCCGCCGCCTGCGTGTACTTTGGC110.218992633884133No Hit
CACCAGCCACCAGTGAACAGGAAGCAGAACATCAGAGAGCCATGCAGAGACGTGCTATCCGTGATGCCAAA110.218992633884133No Hit
GTATCAGTCAGTGCTGGAGAAATCTTGAATAGCTTATGTACAAAACTTTTTAAATTTTATATTATTTTGAA110.218992633884133No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTG100.1990842126219391No Hit
GGTTTAATTTTCTCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.1990842126219391No Hit
CTGTGGGGCTCACCCCAAAGTATTAAAAGTAGCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.1990842126219391No Hit
GCTACTGGAAGGGGTAGGGAACTGACGGCGGATCACTTCAGCCCAGAAATTAGAGGTTGCAGTAAACTATA100.1990842126219391No Hit
GCTACTGCGAATTGATGACATCGTTTCAGGCCACAAAAAGAAAGGCGATGACCAGAGCCGGCAAGGCGGGG100.1990842126219391No Hit
CTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAAAC100.1990842126219391No Hit
GGCGGAGGTTGCAGTGAGCTGAGATCCCGCTACTGTACTTCAGCCTGGGCAAGAGAGTGAGACTCCATCTC100.1990842126219391No Hit
GCCTATGTCGAGTGGCGCCCATGGCGAAGAGGGCTCAGCTCGCATGTGGAAGACTCTCACCTTCTTCGTCG90.17917579135974518No Hit
CCCCATGTGCCTGCACCAGGAGACAGACAGAGAAGGCAGCAGGCGGCCTTTGTTGCTCAGCAAGGGGCTCT90.17917579135974518No Hit
CACCAGGACAGCTGCCACCTCTAAACCAAGTAGAATTCTCTGTGAAATGGGAGCGCCGTAGAATTTTAAGT90.17917579135974518No Hit
TGTGGTCCCAGCTACTCAGGAAGCTGAGGCAGGAGGATTGCTTCAGCCCAGGAGTTCGAGGTTACGGTGAA90.17917579135974518No Hit
ATTAAAAGCTCAATATTTTCTATGAATTCAAAAATACTTCAGAGCCAAAGCCAACTTCAAATACCGTGACC90.17917579135974518No Hit
CATTGATGGAGATGAAGATTTGCCGGGACCTCCAGTAAGATACTACTTCAGTCATGACACTGATTAATACA90.17917579135974518No Hit
GGATTTAAACTTGCTGAATGTAAAGATTGAACCTCAAGTCACTGTAGCTTTAGTAATTGCTTATTGTATTA90.17917579135974518No Hit
TCTTTAATTTGTAAAGAAATAAAATAAATTAAGATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.17917579135974518No Hit
CGGCAACAGCGGAATATTCCGATTCATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTT90.17917579135974518No Hit
GTCTGAGACCAATGACACCAAAGTTCTGAGGCATTAAGCCAGCAGAAGCAGGGTACCCTTTGGGGAGCAGG90.17917579135974518No Hit
GACATAACAGTAACACGATGTACATTTACAAGCGGCCTTATGTACATTTCCCAACAATCTTTTTAAGGCAA90.17917579135974518No Hit
AATTCATACAGATCGTTTAAATAAATGAACATCATTAAAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.17917579135974518No Hit
AAGTCACAACTCTGAAGAGATTTTAAATTTGAAAAGATTTCCCAACATAGATCAAAATCAATTTAGTAAAC90.17917579135974518No Hit
GTGCTGTCTCCATGTTTGATGTATCTGAGCAGGTTGCTCCACAGGTAGCTCTAGGAGGGCTGGCAACTTAG90.17917579135974518No Hit
GAGTAAGGAGCGCTCCCTACTGCATGGGGACTTCCACGCGTTCTCCGCTGGGCCTGGACTCTTCTCCTATA90.17917579135974518No Hit
GTGCAGAATGGGAAGCACTTCAAGTTCACCATCACCGCTGGGTCCAAAGTGATCCAAAACGAATTCACGGT90.17917579135974518No Hit
TAAACAGACAAATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.15926737009755126No Hit
GCTGTTGCTAGTACCTGTTATTTATTACCTGGAGGCCTGTCCAGCACCCACCCTACCCCCATAAAGCATTG80.15926737009755126No Hit
AGTGATGAGATGAATGTGAAGGTGCTGGACTTTGAGCACTTTCTGCCCATGCTGCAGACAGTGGCCAAGAA80.15926737009755126No Hit
CGGCAGAAGGTGGATCTTGGGATCAATTTTTATAAAAATCGAGACAGTTCTGTGGTTAAATCTACAAATTA80.15926737009755126No Hit
CGTTCCTTGTTGCCACTTTGGGTATTCTGAGATTTTCTCTTGCATGCCCTTAGCTTTACAGCTTTTGCATT80.15926737009755126No Hit
GCCTTCTACCTTGTTTGAAACCTGAATCCTAATAAAGAATTAATAACTCCCAAAAAAAAAAAAAAAAAAAA80.15926737009755126No Hit
GTGCAGCTGATATGAATGTCTTGAAGAGTCATGTGGAACAACTTGTCCAAAATCAATTCATATGAAAGCTT80.15926737009755126No Hit
CTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCTAAACCAGAAGAGGAGGTTGCC80.15926737009755126No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA70.13935894883535735No Hit
TCCCTACACGACGCGGGTTTAAATAAATGTAAAAACTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13935894883535735No Hit
GTCATGCCGGTGGTGGTGTGCACATTGAGCCCCGGTATAGACAGTTCCCCCAGCTGACCAGATCCCAGGTG70.13935894883535735No Hit
TGGTAGCCGGCACCTACAGTCCCAGCTACTCAGGAGGCTAAGGCAGGAGAATGGCGTGAACCCAGGAGGCG70.13935894883535735No Hit
GGCTGAGATCGCCACCTACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGTGATGCCTTGGACA70.13935894883535735No Hit
GAAGAAAAGAGACGGGTTGAAGAGGAGAAGAGGAAGAAGAAAGAGGAGGCGGCCCGGAGGAAACAGGAACA70.13935894883535735No Hit
GGGCGAGTATCTCTTCGAAAGGCTCACTCTCAAGCACGACTAAGAGCCTTCTGAGCCCAGCGACTTCTGAA70.13935894883535735No Hit
CTCATGAGGTGGAGGCTGCAGTGAACCGAGATCATGCCACTGCACTCCAGCCTGGGCGAGAAAGCAAGACT70.13935894883535735No Hit
GGATAATGAAATAATCAGTGACTGAAACCATTTTCCCATCATCCTTTGTTCTGAGCATTCGCTGTACCCTT70.13935894883535735No Hit
GTTTTAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13935894883535735No Hit
GTATTTTCTGTATAATTTTACTGCAATTTATTAAATTCTAATGACTTAAAAAAAAAAAAAAAAAAAAAAAA70.13935894883535735No Hit
ATATAAAGCCAACCATTTTTGAGGACCATGTACCTAGTGCTTTGAAAACTCTAAGTCACTATATGAATATG70.13935894883535735No Hit
TTTATATATATTTCTAGACAATAAAGATTTGTTTGTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13935894883535735No Hit
CATCCAAACTGTATCTTCCTTTACTGTTTATATCTTCACCCTGTAATGGTTGGGACCAGGCCAATCCCTTC70.13935894883535735No Hit
ATTTATAATAGAGCTTTTATATAAAGTTTTAAAAATTGAATGTGTGATATGTGTTCTTTGAAGGTTTTTTA70.13935894883535735No Hit
GTACAAGTCTTTCTGCCGGGACCGCATCGCCGATGCCTTCTCCCCGGCCAGCGTCGACAACCAGCTCAATG60.11945052757316346No Hit
CTCAGAGGCAGTAGGAGCTGAGCAAGCTTTGGTGCGGCCTCGGGGCCGAGCTGTGCCCCCCTTCGTATTCC60.11945052757316346No Hit
GCGGAAGACCTGAAGAGTGGTCAGAACAGAGCCTTTGATTTTTATTATGGTTATTTTATTGATTATTACTG60.11945052757316346No Hit
GTGGTACTCTTGCCAGTCCTTGACATTGTCGGGCTTTTCACATGTTGGTAATATTTATTAAAGATGAAGAT60.11945052757316346No Hit
GTTGGGATGAATTTCAAAACGCCTCGGGGACCTGTTTGAATTTTTTCTGTAGTGCTGTATTATTTTCAATA60.11945052757316346No Hit
GATTTTAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11945052757316346No Hit
CTCCTGACCCCTCCCTGCAGGGGCTACGTTAGCAGCCCAGCACATAGCTTGCCTAATGGCTTTCACTTTCT60.11945052757316346No Hit
TTTCTGTCCCCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11945052757316346No Hit
TTCCCTACACGACGCGGGAGGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11945052757316346No Hit
TTACAAAACAGACTCTTTACCTTCTGCTGTGTTTGAAGTATGTTTAGTCAGCATGCTCAGGAAATAAATGT60.11945052757316346No Hit
ATGCTGGACCCAGTAAGATGAGGAAGTCGGGCAGTACACAGGAAGAGGAGCCAGGCCCTTGTACCTATGGG60.11945052757316346No Hit
TTTGTATCATGTGACTTATTAAAATCAGTTTCTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11945052757316346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)