FastQCFastQC Report
Tue 7 May 2024
EGAF00002876194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88156
Total Bases5.8 Mbp
Sequences flagged as poor quality0
Sequence length66
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56496.407958618812105No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4870.5524297835654975No Hit
GGCAGTAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG1400.15880938336585146Illumina Single End PCR Primer 1 (100% over 41bp)
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA1180.13385362312264623No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)