FastQCFastQC Report
Tue 7 May 2024
EGAF00002876216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391
Total Bases355.8 kbp
Sequences flagged as poor quality0
Sequence length66
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88516.416249304396217No Hit
GTATTAGACATACTGTTTTTATAATGTTTTACTTCTGCCTTAAGATTTAG180.333889816360601No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170.3153403821183454No Hit
CCTACAGACGGAGTGCTGTGCCACCTGGTGCCGACAAGAAAGCCGAGGCT130.24114264514932293No Hit
TTCAGGTAGCATCTGAAATTTTAATGAATGTATAATTGGCATATCATGGA120.22259321090706735No Hit
CTATTACGGTTTGCGATAGTATTTCTCTCTAGTTCTCAGTGATTTAAATG120.22259321090706735No Hit
CCTATACGGAGCACGCCAAGCGCAAAACTGTCACAGCCATGGATGTAGTA100.1854943424225561No Hit
ATGTGAAGAGAAGAGAGAATGATTGAAAATGTTTAGTATAGAACTCTTCT100.1854943424225561No Hit
CTGTATTAGACATACTGTTTTTATAATGTTTTACTTCTGCCTTAAGATTT100.1854943424225561No Hit
CCCCATCTCTACTAAAAAAAATACAAAAAATTAGCCGGTGTGGTGGCAGA90.1669449081803005No Hit
CCTATCTACCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC90.1669449081803005Illumina Single End PCR Primer 1 (100% over 40bp)
ATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAAT90.1669449081803005No Hit
TTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATT90.1669449081803005No Hit
TGCCAGGACTGTGGAGCGGATAGGGGGAGTAGGAGTAGAGAAGGGAACAA90.1669449081803005No Hit
GACATTTACTTGGGTGACTTGACATTAAAGGGAAGAGATTGCCTAGAGCA80.1483954739380449No Hit
TAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCC80.1483954739380449No Hit
ATGTTTTACTTCTGCCTTAAGATTTAGGTTTTTTAAATGTATTTTTGCCC70.12984603969578928No Hit
GTGTCCAACAGCTGAAGGAATTTGAGGGGAAGACTTTAGTGTCAGTCACC70.12984603969578928No Hit
ACACGACGCGGGAGGTGTCCAGAAATGGAGTTCTGTGTTTGAGTTGTGCT70.12984603969578928No Hit
GAGAAGAACCAAGCTGGGTCTATAAGGAATTGCACATGAGATGGCACACA70.12984603969578928No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC70.12984603969578928No Hit
CTGCAGAACTGCCCATTCTCATTACTGTGTCCTATTTTATTCATGCCTGT70.12984603969578928No Hit
GTAATAATAGTTTGATTTTTTTATTTCGAGAGAATCTTTCATTTTCCTAT70.12984603969578928No Hit
ACCACTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTT70.12984603969578928No Hit
GATTCACCAAATAAGCTATATACTTTGGTTACCTATGTACCTGTTACCAC70.12984603969578928No Hit
GACTTTGTCATGCTGAAAGGCTGTGTGGTGGGAACCAAGAAGCGGGTGCT70.12984603969578928No Hit
CTCTTACAGGTCACTGTCTTTTATGGTGGTGAACGAAAACTATAATAATA70.12984603969578928No Hit
ATCTAACAGTGAAGAAAATTTTTGTTGGTGGTATTAAAGAAGATACAGAA70.12984603969578928No Hit
GGGCATTACAAAACCGGCTATTCGCCGTTTGGCTCGGCGCGGTGGCGTCA70.12984603969578928No Hit
CTTAAAAATCTGACATTTTACAGTCTGTATTAGACATACTGTTTTTATAA70.12984603969578928No Hit
CCCCCATTACTTCAGTCAAGCCCAAATTTCATCCTCATCTGTTACCTATC70.12984603969578928No Hit
GGCTGGGGCTGTGCGGCCAATGATGGATTTGTTTCTTTTATGTTTTAAAA70.12984603969578928No Hit
CATTAGGCTCAGCAAATTACCTGGGCTGTACTGCCGCAAGGCTTCACAGA60.11129660545353368No Hit
GAGGTGGGGAGTGTGGTGTGTAAAGTCTCTGGCCTCATAAAAGGTGGCTG60.11129660545353368No Hit
GCGCGGTGGCTCCCGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGG60.11129660545353368No Hit
GTAAAGATCTCAAAAAAGAGTAAAGTTGGAATTCTTCCATTTGTTGCTGA60.11129660545353368No Hit
GGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGT60.11129660545353368No Hit
CCTATAATCCCAGGTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAACC60.11129660545353368No Hit
TGTCAGAGTCATTATTATGATTAATATCAATTACGATGCCAAAAATTGCT60.11129660545353368No Hit
GCTAATCATGTGGGTGAGCCAGTGGAACAGCGGGAGACCTGTGCTAGTTT60.11129660545353368No Hit
GTGATGGAGGGGCCTGGAAGCCTCATCCTTCTTGATTGCCACGCGGTGAG60.11129660545353368No Hit
GTGTAATACATTGCCTTAATTGTAAAGCAGCAGAATGAATCAATGTAGGT60.11129660545353368No Hit
GATATATCTAGCTTTACCTCCCGAGTTAAAAAAAAAATCTTTTTATTTTA60.11129660545353368No Hit
ATCTATATGATTGAGCCCATTGATGAGTACTGTGTCCAACAGCTGAAGGA60.11129660545353368No Hit
GGGCACAGCATGCGGAAAATACTACAGAGTGTGCACACTGGCTATCATTG60.11129660545353368No Hit
GATGCAGAGTCAGAAGATGAAGAGGAGGAGGATGTGAAACTCTTAAGTAT60.11129660545353368No Hit
ATTTTATGGATGTAATGTCTATTACGGTTTGCGATAGTATTTCTCTCTAG60.11129660545353368No Hit
TGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTG60.11129660545353368No Hit
GTATTTGTTAATGTGGAGAAAGTAGCAAATGAAAGCCAGAAGACCCCCAG60.11129660545353368No Hit
CAGCCACACCAAACCCTACGTCCGCTCCAAGGGCCGGAAGTTCGAGCGTG60.11129660545353368No Hit
CCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTCA60.11129660545353368No Hit
CCTATGAATTTGGCAAACTGGTTTCCTCCACCAAGAATGAGAACAAAAAG60.11129660545353368No Hit
GTGTCCAACAGCTGAAGGAATTTGAGGGGGAGACTTTAGTGTCAGTCACC60.11129660545353368No Hit
GTCAGAAGATGAAGAGGAGGAGGATGTGAAACTCTTAAGTATATCTGGAA60.11129660545353368No Hit
CTTCTGCAAAAAGAATTGAACCTGCGGAAGAGCTGAACAGCAACGACATG60.11129660545353368No Hit
TTATCGAATGAATTGAATGAAATCATAATCGAATGGAGTCGAATGGAATC60.11129660545353368No Hit
ATTTGATGGAAACTCTGCTTTTAAAATCATCATTTACTGGGTTCTAATAA60.11129660545353368No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)