FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002876216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391
Sequences flagged as poor quality0
Sequence length66
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86816.10090892227787No Hit
GTATTAGACATACTGTTTTTATAATGTTTTACTTCTGCCTTAAGATTTAGGTTTTTTAAATGTATT180.333889816360601No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.24114264514932293No Hit
TTCAGGTAGCATCTGAAATTTTAATGAATGTATAATTGGCATATCATGGAAAATTAACCAGAAAGT120.22259321090706735No Hit
CTATTACGGTTTGCGATAGTATTTCTCTCTAGTTCTCAGTGATTTAAATGTGACCAAGCCTTCTGT110.20404377666481174No Hit
CTGTATTAGACATACTGTTTTTATAATGTTTTACTTCTGCCTTAAGATTTAGGTTTTTTAAATGTA100.1854943424225561No Hit
ATGTGAAGAGAAGAGAGAATGATTGAAAATGTTTAGTATAGAACTCTTCTTGCAGTGGGTTGCTAT100.1854943424225561No Hit
CCTACAGACGGAGTGCTGTGCCACCTGGTGCCGACAAGAAAGCCGAGGCTGGGGCTGGGTCAGCAA100.1854943424225561No Hit
CCTATACGGAGCACGCCAAGCGCAAAACTGTCACAGCCATGGATGTAGTATATGCCCTGAAACGTC100.1854943424225561No Hit
ATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTA90.1669449081803005No Hit
TTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGA90.1669449081803005No Hit
TGCCAGGACTGTGGAGCGGATAGGGGGAGTAGGAGTAGAGAAGGGAACAAGGGAGCAAGGGAACAA80.1483954739380449No Hit
TAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACAT80.1483954739380449No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTC70.12984603969578928No Hit
ATGTTTTACTTCTGCCTTAAGATTTAGGTTTTTTAAATGTATTTTTGCCCTGAATTAAGTGTTAAT70.12984603969578928No Hit
GTAATAATAGTTTGATTTTTTTATTTCGAGAGAATCTTTCATTTTCCTATGTATGCTTTTTTCCTT70.12984603969578928No Hit
ATCTAACAGTGAAGAAAATTTTTGTTGGTGGTATTAAAGAAGATACAGAAGAATATAATTTGAGAG70.12984603969578928No Hit
GACATTTACTTGGGTGACTTGACATTAAAGGGAAGAGATTGCCTAGAGCAGGGGGCAGCACACTGT70.12984603969578928No Hit
CTTAAAAATCTGACATTTTACAGTCTGTATTAGACATACTGTTTTTATAATGTTTTACTTCTGCCT70.12984603969578928No Hit
ACACGACGCGGGAGGTGTCCAGAAATGGAGTTCTGTGTTTGAGTTGTGCTGAATGAAATCTCTTCT70.12984603969578928No Hit
ACCACTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAAATCAGTACTTTT70.12984603969578928No Hit
CTCTTACAGGTCACTGTCTTTTATGGTGGTGAACGAAAACTATAATAATAAATTAATTAAAGGTTT70.12984603969578928No Hit
CCCCCATTACTTCAGTCAAGCCCAAATTTCATCCTCATCTGTTACCTATCTCAGCATAATTCTCAT70.12984603969578928No Hit
GGCTGGGGCTGTGCGGCCAATGATGGATTTGTTTCTTTTATGTTTTAAAATAGGATTTAAAAACTC70.12984603969578928No Hit
GTGTCCAACAGCTGAAGGAATTTGAGGGGAAGACTTTAGTGTCAGTCACCAAAGAAGGCCTGGAAC70.12984603969578928No Hit
GAGAAGAACCAAGCTGGGTCTATAAGGAATTGCACATGAGATGGCACACATATTTATGCTGTCTGA70.12984603969578928No Hit
GCGCGGTGGCTCCCGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATGAGTTGAGGTC60.11129660545353368No Hit
CTGCAGAACTGCCCATTCTCATTACTGTGTCCTATTTTATTCATGCCTGTGTGTTTTTCTTAAGTA60.11129660545353368No Hit
CCTATGAATTTGGCAAACTGGTTTCCTCCACCAAGAATGAGAACAAAAAGAGAAGAAATCCGAAAC60.11129660545353368No Hit
GATTCACCAAATAAGCTATATACTTTGGTTACCTATGTACCTGTTACCACTTTCAAAAATCTACAG60.11129660545353368No Hit
TGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTC60.11129660545353368No Hit
GTATTTGTTAATGTGGAGAAAGTAGCAAATGAAAGCCAGAAGACCCCCAGGGATGTGGTTATGATG60.11129660545353368No Hit
CCCCATCTCTACTAAAAAAAATACAAAAAATTAGCCGGTGTGGTGGCAGACGCCTGTATTCCCAGC60.11129660545353368No Hit
GTGTAATACATTGCCTTAATTGTAAAGCAGCAGAATGAATCAATGTAGGTGCATTCAATTTTTCTT60.11129660545353368No Hit
ATTTTATGGATGTAATGTCTATTACGGTTTGCGATAGTATTTCTCTCTAGTTCTCAGTGATTTAAA60.11129660545353368No Hit
GACTTTGTCATGCTGAAAGGCTGTGTGGTGGGAACCAAGAAGCGGGTGCTCACCCTCCGCAAGTCC60.11129660545353368No Hit
ATTTGATGGAAACTCTGCTTTTAAAATCATCATTTACTGGGTTCTAATAAATTAAAAATTAAACTT60.11129660545353368No Hit
GTGTCCAACAGCTGAAGGAATTTGAGGGGGAGACTTTAGTGTCAGTCACCAAAGAAGGCCTGGAAC60.11129660545353368No Hit
GTGATGGAGGGGCCTGGAAGCCTCATCCTTCTTGATTGCCACGCGGTGAGGGCCACAGGCATGCGC60.11129660545353368No Hit
GAGGTGGGGAGTGTGGTGTGTAAAGTCTCTGGCCTCATAAAAGGTGGCTGTGGGTCGTCAGGAATC60.11129660545353368No Hit
CCTATAATCCCAGGTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAACCTGGGAGGCAGAGGTTG60.11129660545353368No Hit
ATCTATATGATTGAGCCCATTGATGAGTACTGTGTCCAACAGCTGAAGGAATTTGAGGGGAAGACT60.11129660545353368No Hit
GGGCACAGCATGCGGAAAATACTACAGAGTGTGCACACTGGCTATCATTGATCCAGGTGACTCTGA60.11129660545353368No Hit
GATGCAGAGTCAGAAGATGAAGAGGAGGAGGATGTGAAACTCTTAAGTATATCTGGAAAGCGGTCT60.11129660545353368No Hit
GTCAGAAGATGAAGAGGAGGAGGATGTGAAACTCTTAAGTATATCTGGAAAGCGGTCTGCCCCTGG60.11129660545353368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)