FastQCFastQC Report
Wed 8 May 2024
EGAF00002876262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173166
Total Bases12.2 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG22641.3074160054514166Illumina Single End PCR Primer 1 (100% over 45bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9940.574015684372221No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6630.3828696164374069No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)