FastQCFastQC Report
Tue 7 May 2024
EGAF00002876289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61690
Total Bases4.3 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8241.3357108121251418No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2040.33068568649700114No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT1380.22369914086561843No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG1320.21397309126276542No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG1270.20586804992705462No Hit
ACTATAAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG1250.2026260333927703Illumina Single End PCR Primer 1 (100% over 41bp)
TTCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGG970.15723780191278974No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA950.15399578537850545No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA700.11347057869995136No Hit
GCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGA700.11347057869995136No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGC690.11184957043280921No Hit
GTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAA660.10698654563138271No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT630.10212352082995624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)