FastQCFastQC Report
Wed 8 May 2024
EGAF00002876319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420349
Total Bases29.8 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39300.9349373972579927No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA16210.38563193917435273No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6630.15772607999543237No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA6560.156060797099553No Hit
TCCCTACACGACGGCCAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG6290.14963756307258969Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)