FastQCFastQC Report
Tue 7 May 2024
EGAF00002876327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67152
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6550.9753990945913747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5920.8815820824398379No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA2630.39164879675959013No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG950.14147009768882535No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC860.12806766738146294No Hit
TCCCTACACGACGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.10870860138193948Illumina Single End PCR Primer 1 (95% over 22bp)
TCCCTACACGACGCTCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.10573028353585896Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)