FastQCFastQC Report
Tue 7 May 2024
EGAF00002876377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292
Total Bases304.7 kbp
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA2435.661696178937558No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1463.401677539608574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG471.0950605778191984No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC431.0018639328984158No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG240.5591798695246971No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA220.5125815470643057No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA200.4659832246039142No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT160.3727865796831314No Hit
GTAGTTGGAAACCTTGCTGGTGTATGTGATGTGCTTCTGTGCTTTTGAAT100.2329916123019571No Hit
GTTCTATATTTCTACATAGTTGAATTATTTTATCATGCTGTTGCTGGGGA100.2329916123019571No Hit
GGAAGAGTCGGAGGAGTCGGACGAGGATATGGGATTTGGTCTCTTTGACT90.2096924510717614No Hit
ATGCTGTTCTTCTCTCCTAAGTTGAAAATCAGAAACTTGTGGAAAAGAGC90.2096924510717614No Hit
ATTTTACACATATTTTTCTACTGTCCAGTGGAAATCATTTTCTGTTTTGG90.2096924510717614No Hit
TCTGCAGATGGCTAGGGAGTGTGGAGCTGGTACATTTGTCAATTTTGCAT90.2096924510717614No Hit
CTTCAGGAGGAAGCAGCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAA80.1863932898415657No Hit
GCTGAGGAGAAGAAAGTGGAAGCAAAGAAAGAAGAATCCGAGGAGTCTGA80.1863932898415657No Hit
CTTTCTGGTTATATACTGCTTATATCTGTGGATTCAAGTTACTGAAGTGA80.1863932898415657No Hit
GCATAACAGGGTCTCCTTGGCAGCTGTATTCTGGAGTCTGGATGTTGTTC80.1863932898415657No Hit
CCTTGTGGGTGATGATCACTGTGCTGCTTGCGGCTCATGGCAGAGCATTC80.1863932898415657No Hit
CAGTAAGGATGTTAATGTAAATTTTGAAAAATCCAAACTTACATTCAGTT70.16309412861137No Hit
GGCAGGTGCTCAGATGGAAGTAGATTATACAGAGCAACTGAATTTCAGTG70.16309412861137No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAA70.16309412861137No Hit
GTCTAGAAGTATTGCGTTTAGCCATTTTGAGTACAGCATTAAATTGAGGA70.16309412861137No Hit
CTGTTGCTGTATTGATTAGGAAAAGAGACAGAGTAATTTGCAGTTTGTTT70.16309412861137No Hit
GAGTCGACCCTGCACCTGGTCCTGCGCCTGAGGGGTGGCTGTTAATTCTT70.16309412861137No Hit
GGCTGCGGTGGGCAGCCATGAGAACAAAACCTCTTCTGTATTTTTTTTTT70.16309412861137No Hit
GACCACTGAACCAGACAGCGAAGCCAAGATCATTGTTCTACTTTGTATTT70.16309412861137No Hit
GCCTTGATACATCATCAAAGGCATAGCCTTTCCAAGATGGTTTATCAGAC70.16309412861137No Hit
ACTTATAACCCACTTTAAAATATCTACATTAATATACTTGAATGAAAATG70.16309412861137No Hit
TTTTATACTCTATGTTTCTGTTTATTTTTAAATCCTTAACAGCAAAATAT60.13979496738117428No Hit
GTGGTGTTGAGCTGATTATTAACAGTTACTGAAATCAAATATTTATTTGT60.13979496738117428No Hit
GCTGATTAATGGTTTTGCAACTGTAATAGTAGCTGTATCGTTCTAATGCA60.13979496738117428No Hit
GAAAAAAAATGATTTATTGGACTTTGTTAAGATTAAAAACTCTTATTCTT60.13979496738117428No Hit
CCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATA60.13979496738117428No Hit
GGCCTTCCGGGCAGATGACACCTTTGAGGCCCTGTGCATCGAGCCGTTTT60.13979496738117428No Hit
ATGCTAAACTGAAGAAAGAAATTGAAGAACTGAAACAAGAGCTAATTCAG60.13979496738117428No Hit
CCCCCCCAATCGGACTGCCAAATTCTCCGGTTTGCCCCGGGATATTATAG60.13979496738117428No Hit
GTCTCTACTAAAAATACAAAAATTAGCTGGGCATGGTTGCAGGTGCCAGG60.13979496738117428No Hit
GAGTAATCTTTGTAACAAACAGTGCTGGAACAATTGCATAGTCATATGTA60.13979496738117428No Hit
GCTTATCTAATTAACTGTGTTTGGAACTGCTATAAATACATCAACAACCG60.13979496738117428No Hit
ACAAAGACAGGGAGGACAAGAGGAGCGCCCTGTCCTGTGTTATCTCCAAA60.13979496738117428No Hit
CTAACATTTTTGGAAAATTTTCTTAATTTCTTTTGATTAAATTATCTTGC60.13979496738117428No Hit
GCTGGCAAAGGAGAAGTAACTTTTGAGGATGTTAAGCAAGTTTTTGGACA60.13979496738117428No Hit
TTCCCTACACGACGCGGGAATGGAAGCGATTCTCCCATGCTCATGTGTAA60.13979496738117428No Hit
GCTACTTGGGAGGCTGAGGCAGGAGACTCGCTTGAACCTGGGAGGCAGAG60.13979496738117428No Hit
GCTAATCTTGGGAATAAGAGGAGGAAGCGGCTGGCAACTGAAGGCTGGAA60.13979496738117428No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAA60.13979496738117428No Hit
GTTTTAAACGACTTTTGTTTGCGGGGCTTTTAAAAGGAAAACCGAATTAG60.13979496738117428No Hit
CTATTGAAATGGCATCAACATGATGCTGCCCATTCCACTGAAGTTCTGAA60.13979496738117428No Hit
CTGCCCATGTACCAGCAGCTTCAGAAAGAACTGGCGGGGGCACACAGAAC60.13979496738117428No Hit
GTACAATGTAACTATCAAAGTTTTATAATTCATTATGAGTTAACCATTTT60.13979496738117428No Hit
GCTCCAGGGCTACCTGCGATCTGTGTTTGCTCTGACGAATGGAATTTATC60.13979496738117428No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT50.11649580615097856No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA50.11649580615097856No Hit
AGAGAAAGAAGGTTAAGCTGGCTGTCCTGAAATATTATATGGTGGATGAG50.11649580615097856No Hit
GCCGCCTCCGGGAGAGCTCCCGCCTCATGTTTGAATACCAGACATTACTG50.11649580615097856No Hit
GGATATGAGGGTAATAATCTGAAACTCAGTTGCTTTCCTGTAGACCATCA50.11649580615097856No Hit
GGTTACTGGAGGGCCTTTCAGCCCTCTCCTTGGCACAAGAAGTATGTCAG50.11649580615097856No Hit
CCCCTTTGGATATCAGACTTAAAGAGGGTTGCTCTCCTTTCTTGGACCAA50.11649580615097856No Hit
GTGTAATGTACACATATGGGAATAAAGGAAGGGGAAGAAAGAGAGAAAGG50.11649580615097856No Hit
GCAGATATAATGGGGAGCCTGAGCATATTGAGAGAAATGACGCACCTCAT50.11649580615097856No Hit
CTATATTGTTTAACTTTGTAAGAATGCCCATTACTTTTTTAACTAGTAAA50.11649580615097856No Hit
GACCAACTTTAAATGAACTGGGAATCTCCACTCCGGAGGAACTGGGCCTT50.11649580615097856No Hit
GCCCTACAAAGGCTAGAACACTACAGGGGATGAATTCTTCAAATAGGAGC50.11649580615097856No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCA50.11649580615097856No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA50.11649580615097856No Hit
GGATCTAGTGTTATCGGTATAGGTGTAAAGAAGAGTTTGAGGTTTATTAT50.11649580615097856No Hit
CCTTAAAATGCTTAACACATATTTGTTGAATGAATAAGTGCCCCCCAGTG50.11649580615097856No Hit
GGGAAAATGGGGCTGGAGATAGCAGGGTAGAGATAAGGCAGAGCAAAAAT50.11649580615097856No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT50.11649580615097856No Hit
ATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGC50.11649580615097856No Hit
AAACCCGTCTCTACAAAAACTACAAAAATTAGCCGGGTGTGGTGACTCAC50.11649580615097856No Hit
GTGATATCTGCAAAATCCTGAATATTAGAAGATCAGAAGAGCTTTCCTTG50.11649580615097856No Hit
CTGTCATCTGTGTTTAGCTCGGGGAGTGCCCCCTAAGGGGGCGAACTGAC50.11649580615097856No Hit
CTGATATTCCTGTGGTGTGTCTAGAATCAGATAATGGGAACATTATGATC50.11649580615097856No Hit
GATGACTGTCAGGATGTTGCTTCAGAGTGTGAAGTCAAATGCATGCCAAC50.11649580615097856No Hit
GGTCTAGAAAACCAGCTTGCGAATAACAGTCCCCGTGGCCATCCCTGTGA50.11649580615097856No Hit
GGGCTATCCAGAAAAAAAGCAAAGCAGGTGATATCTAATTTAGGATTTGA50.11649580615097856No Hit
CCCTCAGCACTTGCTCAGTAGTTTTGTGAAAGTCTCAAGTAAAAGAGACA50.11649580615097856No Hit
GAAGAAACTGTTATAAGTGAAGAACCACCAGCTAAGAGACAATGTATCGA50.11649580615097856No Hit
CCCCACGACCTTGGCCCGAGCCCCTCCGTGAGGAACACAATCTCAATCGT50.11649580615097856No Hit
GCGCCAGTGAAGAAATCTATACGAGATACTCCAGCCAAAAATGCACAAAA50.11649580615097856No Hit
CCATCAGTTGCCATCCACTACCCCGTTTTCTCTTCTTGCTGCAAAATAAA50.11649580615097856No Hit
GTGCATCTGTAACTTCAGGAGGATCACTTGAGCTTTAAAACCTGTGCCTT50.11649580615097856No Hit
GGAAACACAAGAAGAAACGCCTGGTGCAGAGCCCCAATTCCTACTTCATG50.11649580615097856No Hit
GGACCCTACAGTGCCTGGGGCTCCGCTTACAATAGTCCTCCCTCTGTTCC50.11649580615097856No Hit
GGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCT50.11649580615097856No Hit
GGGTCAGTCCTTCCCGTTCTGTGCTAGATTGCTGTATAAGGACCTCACAG50.11649580615097856No Hit
GCTATATACTTTGGTTACCTATGTACCTGTTACCACTTTCAAAAATCTAC50.11649580615097856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)