FastQCFastQC Report
Wed 8 May 2024
EGAF00002876434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054164
Total Bases74.8 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA44630.4233686599049104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26310.2495816590207975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24650.2338345836131759No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT12960.12294102245950346No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC11390.10804770415229509No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT10820.10264057584967803No Hit
GGTTAATACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT10710.101597094949173Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)