FastQCFastQC Report
Wed 8 May 2024
EGAF00002876446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610221
Total Bases43.3 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63611.042409225510102No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA25690.42099501655957433No Hit
GGTATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG16300.26711633981786925Illumina Single End PCR Primer 1 (100% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15750.25810321178720497No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC8730.1430629231049079No Hit
CGGTATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC7160.1173345394537389Illumina Single End PCR Primer 1 (100% over 40bp)
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT6640.10881303658838355No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG6170.10111090899854315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)