FastQCFastQC Report
Wed 8 May 2024
EGAF00002876458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213880
Total Bases15.1 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA24111.1272676267065644No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG6900.32261080979988777No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT5840.27305030858425283No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC5270.24639985038339257No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5130.23985412380774263No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5030.23517860482513558No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC4960.23190574153731064No Hit
GAACCGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG4740.2216195997755751Illumina Single End PCR Primer 1 (100% over 41bp)
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT4600.21507387319992516No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAA4210.19683934916775764No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC3940.18421544791471853No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA3570.16691602767907238No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCA3500.16364316439124743No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA3290.15382457452777257No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC3270.15288947073125117No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA3240.15148681503646905No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA3120.14587619225734058No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG3030.1416682251729942No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT3000.1402655694782121No Hit
CTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAAATCGCTGT3000.1402655694782121No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAA2890.13512249859734432No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA2760.1290443239199551No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT2670.12483635683560876No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC2640.12343370114082663No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2520.11782307836169816No Hit
ATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACACTTCT2500.11688797456517674No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAG2450.1145502150738732No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTAC2440.1140826631756125No Hit
TCCTAATACCAACTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCC2200.10286141761735552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)