FastQCFastQC Report
Wed 8 May 2024
EGAF00002876469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469183
Total Bases33.3 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42610.9081744223469307No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA12500.26642056510998907No Hit
GATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACC11560.2463857386137179No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9100.19395417140007204No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC7190.1532451090512657No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT6540.13939123966554628No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA6100.13001323577367466No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC5750.12255345995059498No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA5610.11956954962136308No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC4750.10123981474179586No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA4710.10038726893344388No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)