FastQCFastQC Report
Wed 8 May 2024
EGAF00002876491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681037
Total Bases48.3 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA107301.5755384802881487No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44560.6542963157655164No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41000.6020230912564222No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA39590.5813193703132136No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT20090.2949913147156469No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACC19160.28133566898714757No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC17570.25798891983842287No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA16520.2425712553062462No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGG15100.2217206994627311No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAA14860.21819666185537642No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAA14360.21085491684005422No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13890.20395367652565133No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGC13430.19719927111155489No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA12950.19015119589684554No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11850.17399935686313667No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT11600.17032848435547557No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG11400.16739178634934668No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG11060.16239939973892756No Hit
GGGTAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAACCTGGTCCACAT10540.15476398492299245No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA10480.15388297552115376No Hit
TTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAG10330.1516804520165571No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTAC9770.14345769759939622No Hit
TTCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGG8410.12348815115771977No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGG8370.122900811556494No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC8210.12055145315159087No Hit
GAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATA7710.11320970813626867No Hit
ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAA7690.11291603833565578No Hit
GGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA7400.10865782622676888No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA7020.10307810001512399No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTG6920.10160975101205956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)