FastQCFastQC Report
Wed 8 May 2024
EGAF00002876499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561810
Total Bases39.8 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66961.1918620174080206No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA51330.9136540823410051No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17150.30526334525907334No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA10290.183158007155444No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC9420.16767234474288462No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA7590.13509905484060447No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT7470.13296310140438938No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC7430.13225111692565103No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG6790.1208593652658372No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT6780.12068136914615263No Hit
ATATAACGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG6530.11623146615403784Illumina Single End PCR Primer 1 (100% over 41bp)
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACC5620.10003381926274008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)