FastQCFastQC Report
Tue 7 May 2024
EGAF00002876560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762
Total Bases125.1 kbp
Sequences flagged as poor quality0
Sequence length71
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40222.8149829738933No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA181.0215664018161181No Hit
AAATAAATGTGTGGACTTAATTCACCCCAGTCTTCATCATCTGGGCATCA170.9648127128263337No Hit
GAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAA150.851305334846765No Hit
GTATTAAAATTTCCTTCATCTCTATAGAAATGGAAAGATTTTTTATAAGT130.7377979568671964No Hit
ATGTATGTCAAGTTGGTGGAGGCCCTTTGTGCTGAACACCAAATCAACCT120.681044267877412No Hit
GATTTAAAGATTTCTCTTTTTCATTCATATACCATTTTATGAGTTCTGTA110.6242905788876276No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG110.6242905788876276No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110.6242905788876276No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATA100.5675368898978433No Hit
GACTGGGATCCTACTGGGGACTCTGGCTGGTGGCCTTTTTGGTGGGTTGG100.5675368898978433No Hit
ACATAGCAAGACCCCATCTCTACAAAAAAATAAAAATAAAAATTAGCCAG100.5675368898978433No Hit
GTATCTCTGGGACACATTCAAAGCAGTTTGTAGAGGGAAATTTATAGCAC90.5107832009080591No Hit
GAATTGCTTGAACCTGGGAGGTGGAGGTTGCAGTGAGCTGAGATCATGCC80.4540295119182747No Hit
CTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTC80.4540295119182747No Hit
AAGGCCAAATTCTTCAGCAGAAGAGCTGAGGAGAAGATTAAGAGTGTTGG80.4540295119182747No Hit
GGGCAGATCACAAGGTCAGGAGATCAAGACCATCCTGGCTAACACGGTGA80.4540295119182747No Hit
AATGTGACTGACTAGCAGCTTCATTTTGAATGAGGGTCGCTGTCTGCCCA80.4540295119182747No Hit
GCCCAAGCTGGAGTGCAGTGGCGCGATGTCGGCTCGCTGCAAGCTCCACC80.4540295119182747No Hit
CCAAGACTAGACCTGGCTAACAAACATAGGAGACAAAGTTAGGAAACATT70.39727582292849034No Hit
CAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATAT70.39727582292849034No Hit
AATGTGTTGAGCCTAACTGCAGATCCAAGAGGATGCTGGCCATTAAGAGA70.39727582292849034No Hit
CACAACTTCTTAAGCTAAATGGTATTTTCATTTTTCTCAAGCTCTCCAAT70.39727582292849034No Hit
TTTTAAGGCTGGGCATGGTGGTTCACGCCTGTAATCCCAGCACTTTGGGA70.39727582292849034No Hit
GCAGAGCCAGATACAGGAGGCCCGAGCCGACTTAGCCCGCAGGGGGCTGC70.39727582292849034No Hit
CTAAAGAACTTGCCACTAAACTGGGTTAAATGTACACTGTTGAGTTTTCT60.340522133938706No Hit
ATTTTGTAATATAAGTTTTGAAATCTAAATGTCAATTTTCTACAAATTAT60.340522133938706No Hit
GGTGAGAGATGAGTACTTTCATATTTCTGAAATCCTGAGATTCAGGCAAA60.340522133938706No Hit
CACAAAATTAGCGAGGTATGGTGGCTCATGCCTGTAATCCCAGCACTTTG60.340522133938706No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.340522133938706No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGA60.340522133938706No Hit
GTATTGGGAAATGTGAAAATAAAATTGTCAACTCTTTCAATCAAAAAAAA60.340522133938706No Hit
CCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAA60.340522133938706No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC60.340522133938706No Hit
GGGAGGCCAAAGTGGGAGGATCGCTTGAGCCCAGAAGTTCAAGACCAGCC60.340522133938706No Hit
TGCTCATAAAAGTGACTTTCAGTAACATTTTCAGTATAAAAATGGATTTA50.28376844494892167No Hit
CCTCACAATCATGGCAAGCCAACGCCACTTATCCAGTGAACCACTATCAC50.28376844494892167No Hit
GTATTAGGGAAAGGTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.28376844494892167No Hit
CACTACAAGAAAAACTCCTTGTGGTGAAGGTTCTAAGACGTGGGATCGTT50.28376844494892167No Hit
ATTTTAAAACCGCATACATAATAAAAGACATTGTTGCATGGCTAAAAAAA50.28376844494892167No Hit
GGATGCCTCTAGGCCCCCTTCCCTCTACACACCTCTGGGAAAAGATTACA40.22701475595913734No Hit
TTAGAAATCAGTTGGACTTCAACATTCTCAAGTACTTCCACAAAGCATCC40.22701475595913734No Hit
GGATAAATACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.22701475595913734No Hit
GGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGG40.22701475595913734No Hit
GGGTGGATCGCCTGAGGTCAGGAGTTCAAGACCAGCCCGACCAACATGGT40.22701475595913734No Hit
GCCTTAATAAACAGACATTGATTCCCTTAAAAAAAAAAAAAAAAAAAAAA40.22701475595913734No Hit
GTATTGGGAAATGTGAAAATAAAATTGTCAACTCTTTCAGTCAAAAAAAA40.22701475595913734No Hit
CTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTT40.22701475595913734No Hit
GAATTTATCCTCACAAATTGGTGTTCTAAATGTCTTAAGAACCTAATTAA40.22701475595913734No Hit
TATATATGCAGATCTAATGTAAAATCCAGAACTTGGACTCCATCGTTAAA40.22701475595913734No Hit
GTGTTAGCCAGGATGGTCTCGATCTTCTGACCTCGTGATCCGCCCATCTC40.22701475595913734No Hit
GGGAGGAAAAGTAACAATAGTCCACATGTCCCTGGCATCTGTTCAGAGCA40.22701475595913734No Hit
GAGTGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.22701475595913734No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.22701475595913734No Hit
AGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.22701475595913734No Hit
GGGCATTCCTCTTTATAAAGGCATATTAAGGAGACACTTAGAAGGCCAGG40.22701475595913734No Hit
GGCACTGTTCATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTC40.22701475595913734No Hit
CTTTTAATGTGTACAATTAGGAAATTTTTTTAAGTGAGGACTCTCTACCC30.170261066969353No Hit
ACACGACGCGGGGGCAGAAACAGAGCATGGTGGCGGATACTGATGACTTC30.170261066969353No Hit
TCCCTACACGACGCGGGAGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
GAGTTAAGTTTATGATTATGAAATAAAAACTAAATAACAATTAAAAAAAA30.170261066969353No Hit
CCCAAGAACAGGGTTTGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
GTCTCGTCTCTTTTACCCTGGCCCCTCTCCCTTGGGCTCTGATGAAAAAT30.170261066969353No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
GCACTATTTCATTAGTTTTAACAAAACTGTTCCAAAAGCGATTTGAGATG30.170261066969353No Hit
GGCTACAGAAGCTTCCAACAAGCATGTAGGCAGATACTCAAATGACATTC30.170261066969353No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC30.170261066969353No Hit
TCCCTACACGACGCGGGGTCTGGCCTCACGTGTCTCAGATTTTCTAAGAA30.170261066969353No Hit
GTTAAGCACAAAGGAAAACATTTCAATAAAGGATCATTTGACAACTGGTG30.170261066969353No Hit
CGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAACTGGCTTTT30.170261066969353No Hit
TGTCTATTGTTTGTGTGCTTGTTTTGCGCTATGGAACATTTTTTAATTTA30.170261066969353No Hit
CTCTCCATTGCCTGCACCCCACCCCAGCCACTCAACTCCTACTTATTTTT30.170261066969353No Hit
GTGAGACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
GGTATAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
TGGCTTACCTTTCCTGCCTTTGACCCATCACACCCCATTTCCTCCTCTTT20.11350737797956867No Hit
ACCGGGTTTTCCTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
TCCCAGCTACTCGGGAGGCTGAGGCAGAAGAATGGTGAGAATCCAGGAGG20.11350737797956867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGG20.11350737797956867No Hit
GAAAATGACAAAGACATCAAGCACCCAGCATTACTGTTATCTTCCCTGAG20.11350737797956867No Hit
TGTCTGAGACCTGAGAGGTGAGACTGAGCCTGGTGGACCCCTGCAAAGTG20.11350737797956867No Hit
CCTAGGAGGTGGAGGTTGCAGTGGGCAACAGAGAGAGATTCCATCTCAAA20.11350737797956867No Hit
ACTTGAAACAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GACTCACCGCTGTTCGCTCTCGCCGAGGAACAAGTCGGTCAGGAAGCCCG20.11350737797956867No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GTGTGAGAGCAGGTGCCGGCTCCTCACACACCATGGAGACTACCATTGCG20.11350737797956867No Hit
ACACGACGCGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GTGCAGCGGACTCAGGCTCCAGCTGTGGCTACAACATAGGGTTTTTATAC20.11350737797956867No Hit
TGCCTCTGGGCCCTGGGGGCTGGGCTGTAGAAGAATTATTAAAAAAAAAA20.11350737797956867No Hit
GCAGGGCCAGTGTGAGCTTACAGCGACGTAAGCCCAGGGGCAATGGTTTG20.11350737797956867No Hit
CAATGTGTGATCTTTATTTTGAAAAATACAGAACTTTGGAATCTGAAAAA20.11350737797956867No Hit
CCCTTAGAAACATTTTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
TTTTAAGGCTGGGCATGGTGGTTCACGCCTGTAATCCCAGCACGTTGGGA20.11350737797956867No Hit
CCTCATGGGTTTGGCTTAGGCTGGTAGCTTCTATGTAATTCGCAATGATT20.11350737797956867No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA20.11350737797956867No Hit
AAATAAATGTGTGGACTTAATTCACACCAGTCTTCATCATCTGGGCATCA20.11350737797956867No Hit
GCCACTTAGTTCAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GGGTTTATGGCAGAGCTTTACAAATAAAAAAAAAACTAACTTTTCTAAAA20.11350737797956867No Hit
ATGTATGTCAAGTTGGTGGAGGCGCTTTGTGCTGAACACCAAATCAACCT20.11350737797956867No Hit
CGCGGGAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
CAATCCAACTTGAACTCTCTCACTGATTACTTGATGACAATAAAATATCT20.11350737797956867No Hit
CCTCATCTTCCTGCCCCGCTTTCCTCTCCCATCCTCATCCCCCACACTGG20.11350737797956867No Hit
CACAGCACTTCGGCTCCTCTAACCTGTGCCACAGGTGACCACCAATAAAA20.11350737797956867No Hit
TCCCTACACGACGCGGGGTACATAAAAATAATTGAAATAAAAAAAAAAAA20.11350737797956867No Hit
CAATGTGTGATCTTTATTTTGAAAAATACAGAACTTTGGAATCTAAAAAA20.11350737797956867No Hit
CCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
CTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCACTTGAACCT20.11350737797956867No Hit
GCTCTTCTCCTGACCTGCCAATAAAAATTTATGGTCCAAGGGAAAAAAAA20.11350737797956867No Hit
ATAAAACTCAGTGCTGTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GTCCTCCATCCTTCTCCCAGGATGGTGAAGGGGGACCTGGTACCCAGTGA20.11350737797956867No Hit
GAGCCGAGATTGCGCCACTGCACTCCAGCCTGGGCGACAGAGCAGGACTC20.11350737797956867No Hit
ATTTTAAAACCGCATACATAATAAAAGACATTGTTGCATAACTAAAAAAA20.11350737797956867No Hit
CATCTACTTAATAAATATTACAGTGAATTACCTGTCTCAAAAAAAAAAAA20.11350737797956867No Hit
GTATATACTACTGAAATGAATGGCAAAAACACATTCTGGGATGTGGATGG20.11350737797956867No Hit
GTTTTAAAAAGCAAGAGAAATTCACAAACTGCAGCTTTCTAAAAAACAAA20.11350737797956867No Hit
GCCAAATAAACAGCTATTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GTTCCGACCTGCACGAATGGCGTAACGACTTCCCCACTGTCTCCAGGACA20.11350737797956867No Hit
GGTCTATTTGGTGTCGTGGAACCTCTTACTGCTTTCAATACACGATTAGT20.11350737797956867No Hit
ATTTTAAAACCGCATACATAATAAAAGACATTATTACATAACTAAAAAAA20.11350737797956867No Hit
CCATTATGCAGAATCCACGCCAGTACAAGATCCCAGACTGGTTCTTGAAC20.11350737797956867No Hit
CAATGTGTGATCTTTATTTTGAAAAATACAGAACTTTAGAATCTAAAAAA20.11350737797956867No Hit
TCACAGGACCTGGGACTGCACAGGAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)