FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002876560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762
Sequences flagged as poor quality0
Sequence length71
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39322.304199772985246No Hit
AAATAAATGTGTGGACTTAATTCACCCCAGTCTTCATCATCTGGGCATCAGAATATTTCCTTATGGTTTTG160.9080590238365494No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG120.681044267877412No Hit
GTATTAAAATTTCCTTCATCTCTATAGAAATGGAAAGATTTTTTATAAGTCCTCTAATTTGCTTTAAGATA120.681044267877412No Hit
ATGTATGTCAAGTTGGTGGAGGCCCTTTGTGCTGAACACCAAATCAACCTAATTAAGGTTGATGACAACAA120.681044267877412No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT100.5675368898978433No Hit
GAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGA100.5675368898978433No Hit
GATTTAAAGATTTCTCTTTTTCATTCATATACCATTTTATGAGTTCTGTATAATTTTTTGTGGTTTTTGTT100.5675368898978433No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.5107832009080591No Hit
GACTGGGATCCTACTGGGGACTCTGGCTGGTGGCCTTTTTGGTGGGTTGGGTCTTGTGGCAGAAATTTTAC90.5107832009080591No Hit
GTATCTCTGGGACACATTCAAAGCAGTTTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGAGAAAGC80.4540295119182747No Hit
ACATAGCAAGACCCCATCTCTACAAAAAAATAAAAATAAAAATTAGCCAGCAGCCACGGTGGCACACACCT80.4540295119182747No Hit
GGGCAGATCACAAGGTCAGGAGATCAAGACCATCCTGGCTAACACGGTGAAACCCTGTCTCTACTAAAAAT80.4540295119182747No Hit
CACAACTTCTTAAGCTAAATGGTATTTTCATTTTTCTCAAGCTCTCCAATAAATATGACCACCAAGATGCA70.39727582292849034No Hit
AATGTGTTGAGCCTAACTGCAGATCCAAGAGGATGCTGGCCATTAAGAGATGCAAGCATTTTGAACTGGGA70.39727582292849034No Hit
CAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTCAACTTAGTATTATAC70.39727582292849034No Hit
AAGGCCAAATTCTTCAGCAGAAGAGCTGAGGAGAAGATTAAGAGTGTTGGGGGGGCCTGTGTCCTGGTGGC70.39727582292849034No Hit
AATGTGACTGACTAGCAGCTTCATTTTGAATGAGGGTCGCTGTCTGCCCATTGATAGAGGCCAGATTGTCT70.39727582292849034No Hit
CTTTAAGAGTTTAAAATCATCAGAAAGGAAAAACAAACTCTATATTGTTCAGCATGCAATACATACCACGC70.39727582292849034No Hit
GTATTGGGAAATGTGAAAATAAAATTGTCAACTCTTTCAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.340522133938706No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA60.340522133938706No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGACCTACAGAGCTCATGGTCGGA60.340522133938706No Hit
CCAAGACTAGACCTGGCTAACAAACATAGGAGACAAAGTTAGGAAACATTGATACAAGCTTTGTACAGAGA60.340522133938706No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA60.340522133938706No Hit
GGGAGGCCAAAGTGGGAGGATCGCTTGAGCCCAGAAGTTCAAGACCAGCCTGTGCATCATGGAGAAACCCC60.340522133938706No Hit
CTAAAGAACTTGCCACTAAACTGGGTTAAATGTACACTGTTGAGTTTTCTGTACATAAAAATAATTGAAAT60.340522133938706No Hit
CACAAAATTAGCGAGGTATGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATC60.340522133938706No Hit
ATTTTGTAATATAAGTTTTGAAATCTAAATGTCAATTTTCTACAAATTATAAAAATAAACTCCACTCACTA60.340522133938706No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.340522133938706No Hit
GCCCAAGCTGGAGTGCAGTGGCGCGATGTCGGCTCGCTGCAAGCTCCACCTCCTGGGTTCACACCATTCTT60.340522133938706No Hit
GCAGAGCCAGATACAGGAGGCCCGAGCCGACTTAGCCCGCAGGGGGCTGCGCTTCTGACAGCCTAACCCCA60.340522133938706No Hit
CCTCACAATCATGGCAAGCCAACGCCACTTATCCAGTGAACCACTATCACGAAAAAAAAAAAAAAAAAAAA50.28376844494892167No Hit
GAATTGCTTGAACCTGGGAGGTGGAGGTTGCAGTGAGCTGAGATCATGCCACTGCACTCCAGCCTGAGCAA50.28376844494892167No Hit
GTATTAGGGAAAGGTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.28376844494892167No Hit
TTTTAAGGCTGGGCATGGTGGTTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGGATCACC50.28376844494892167No Hit
CACTACAAGAAAAACTCCTTGTGGTGAAGGTTCTAAGACGTGGGATCGTTTCCAGATGAGAATTCACAAGC50.28376844494892167No Hit
GGTGAGAGATGAGTACTTTCATATTTCTGAAATCCTGAGATTCAGGCAAAGTTTTAATAATTGTTTTATAT50.28376844494892167No Hit
CCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTG50.28376844494892167No Hit
ATTTTAAAACCGCATACATAATAAAAGACATTGTTGCATGGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.28376844494892167No Hit
GGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCC40.22701475595913734No Hit
AGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.22701475595913734No Hit
TGCTCATAAAAGTGACTTTCAGTAACATTTTCAGTATAAAAATGGATTTACATTTCATTTCTGGAAAACCG40.22701475595913734No Hit
CTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACGAAAAAAAAAAAAAAA40.22701475595913734No Hit
GGGAGGAAAAGTAACAATAGTCCACATGTCCCTGGCATCTGTTCAGAGCAGTGTGCAGAATGTAATGCTCT40.22701475595913734No Hit
GCCTTAATAAACAGACATTGATTCCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.22701475595913734No Hit
GGATGCCTCTAGGCCCCCTTCCCTCTACACACCTCTGGGAAAAGATTACACTGTATTAACTCTCGAGGAGT40.22701475595913734No Hit
GGATAAATACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.22701475595913734No Hit
GTATTGGGAAATGTGAAAATAAAATTGTCAACTCTTTCAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.22701475595913734No Hit
GGGTGGATCGCCTGAGGTCAGGAGTTCAAGACCAGCCCGACCAACATGGTGAAACCTTGTCTCTACTAAAT40.22701475595913734No Hit
GAATTTATCCTCACAAATTGGTGTTCTAAATGTCTTAAGAACCTAATTAAATAGCTGACTACAGAAAAAAA40.22701475595913734No Hit
TATATATGCAGATCTAATGTAAAATCCAGAACTTGGACTCCATCGTTAAAATTATTTATGTGTAACATTCA40.22701475595913734No Hit
GGCACTGTTCATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTCTGCTGAAAGTTCATATCTGGA40.22701475595913734No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA30.170261066969353No Hit
GCACTATTTCATTAGTTTTAACAAAACTGTTCCAAAAGCGATTTGAGATGCCAATACCTGTGAATACCTGT30.170261066969353No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
GTGTTAGCCAGGATGGTCTCGATCTTCTGACCTCGTGATCCGCCCATCTCGGCCTCCCAAAGTGCTGGGAT30.170261066969353No Hit
TCCCTACACGACGCGGGAGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
ACACGACGCGGGGGCAGAAACAGAGCATGGTGGCGGATACTGATGACTTCAACGTAATATGAAAGGACGTG30.170261066969353No Hit
GGCTACAGAAGCTTCCAACAAGCATGTAGGCAGATACTCAAATGACATTCAGGAACTCTAATATTCATGGA30.170261066969353No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC30.170261066969353No Hit
GTTAAGCACAAAGGAAAACATTTCAATAAAGGATCATTTGACAACTGGTGAAAAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
GAGTTAAGTTTATGATTATGAAATAAAAACTAAATAACAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
CGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAACTGGCTTTTCTGAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
GAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGC30.170261066969353No Hit
GTGAGACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.170261066969353No Hit
TGTCTATTGTTTGTGTGCTTGTTTTGCGCTATGGAACATTTTTTAATTTATTTTAAGATAAATTATTAAGT30.170261066969353No Hit
CCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GAGTGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GCCAAATAAACAGCTATTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
CCTCATGGGTTTGGCTTAGGCTGGTAGCTTCTATGTAATTCGCAATGATTCCATCTAAATAAAAGTTCTAT20.11350737797956867No Hit
GGGCATTCCTCTTTATAAAGGCATATTAAGGAGACACTTAGAAGGCCAGGTGTGGTGGCTCATGCCTGTAA20.11350737797956867No Hit
GTTCCGACCTGCACGAATGGCGTAACGACTTCCCCACTGTCTCCAGGACATGCTCAGCGAAAAAAAAAAAA20.11350737797956867No Hit
GAATTGCTTGAACCTGGGAGGTGGAGGTTGCAGTGAGCTGAGATCATGCCACTGCACTCCAGCCTGCGCAA20.11350737797956867No Hit
GTTTTAAAAAGCAAGAGAAATTCACAAACTGCAGCTTTCTAAAAAACAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
TCCCTACACGACGCGGGGTACATAAAAATAATTGAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GCCACTTAGTTCAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GTCCTCCATCCTTCTCCCAGGATGGTGAAGGGGGACCTGGTACCCAGTGATCCCCACCCCAGGATCCTAAA20.11350737797956867No Hit
CGCGGGAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
CATCTACTTAATAAATATTACAGTGAATTACCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GTGCAGCGGACTCAGGCTCCAGCTGTGGCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAA20.11350737797956867No Hit
ATAAAACTCAGTGCTGTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
TCACAGGACCTGGGACTGCACAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
CAATGTGTGATCTTTATTTTGAAAAATACAGAACTTTGGAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
TCCCAGCTACTCGGGAGGCTGAGGCAGAAGAATGGTGAGAATCCAGGAGGCAGAGCTTGCAGTGAGCCAAG20.11350737797956867No Hit
GCTCTTCTCCTGACCTGCCAATAAAAATTTATGGTCCAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
ATTTTAAAACCGCATACATAATAAAAGACATTATTACATAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
CCTCATCTTCCTGCCCCGCTTTCCTCTCCCATCCTCATCCCCCACACTGGGATAGATGCTTGTTTGTAAAA20.11350737797956867No Hit
TTAGAAATCAGTTGGACTTCAACATTCTCAAGTACTTCCACAAAGCATCCCTTGGTTTTATTCCAGTTTGT20.11350737797956867No Hit
GGTCTATTTGGTGTCGTGGAACCTCTTACTGCTTTCAATACACGATTAGTAATCTAAAAAAAAAAAAAAAA20.11350737797956867No Hit
GTCTCGTCTCTTTTACCCTGGCCCCTCTCCCTTGGGCTCTGATGAAAAATTGCTGACTGTAGCTTTGGAAA20.11350737797956867No Hit
GAGCCGAGATTGCGCCACTGCACTCCAGCCTGGGCGACAGAGCAGGACTCCATCTCAAAAAAAAAAAAAAA20.11350737797956867No Hit
GCCCAAGCTGGAGTGCAGTGGCGCGATGTCGGCTCGCTGCAAGCTCCACCTCCTGGGTTCACACCAGTCTT20.11350737797956867No Hit
CAATGTGTGATCTTTATTTTGAAAAATACAGAACTTTAGAATCTAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
CTTTTAATGTGTACAATTAGGAAATTTTTTTAAGTGAGGACTCTCTACCCTTGCCGTATCTAAGGAGCTGA20.11350737797956867No Hit
GGGTTTATGGCAGAGCTTTACAAATAAAAAAAAAACTAACTTTTCTAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
AAATAAATGTGTGGACTTAATTCACACCAGTCTTCATCATCTGGGCATCAGAATATTTCCTTATGGTTTTG20.11350737797956867No Hit
ACACGACGCGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
TGTCTGAGACCTGAGAGGTGAGACTGAGCCTGGTGGACCCCTGCAAAGTGACATTGAGAAAGATGGCAAGC20.11350737797956867No Hit
GTGTGAGAGCAGGTGCCGGCTCCTCACACACCATGGAGACTACCATTGCGGCTGCATCGCCTTCTCCCCTC20.11350737797956867No Hit
ACCGGGTTTTCCTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
CCTAGGAGGTGGAGGTTGCAGTGGGCAACAGAGAGAGATTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
ATGTATGTCAAGTTGGTGGAGGCGCTTTGTGCTGAACACCAAATCAACCTAATTAAGGTTGATGACAACAA20.11350737797956867No Hit
TGCCTCTGGGCCCTGGGGGCTGGGCTGTAGAAGAATTATTAAAAAAAAAAAAAACTAAAAAAAAAACATCT20.11350737797956867No Hit
CAATCCAACTTGAACTCTCTCACTGATTACTTGATGACAATAAAATATCTGATATTCTACAAAAAAAAAAA20.11350737797956867No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
CAATGTGTGATCTTTATTTTGAAAAATACAGAACTTTGGAATCTGAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
ATTTTAAAACCGCATACATAATAAAAGACATTGTTGCATAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11350737797956867No Hit
TGGCTTACCTTTCCTGCCTTTGACCCATCACACCCCATTTCCTCCTCTTTCCCTCTCCCCGCTGACAAAAA20.11350737797956867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.11350737797956867No Hit
GAAAATGACAAAGACATCAAGCACCCAGCATTACTGTTATCTTCCCTGAGAATGAGACAGAATAATCATGT20.11350737797956867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)