FastQCFastQC Report
Wed 8 May 2024
EGAF00002876601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193181
Total Bases13.7 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18850.9757688385503751No Hit
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7240.3747780578835393No Hit
TCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7110.3680486176176746No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA6020.31162484923465555No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC5440.2816011926638748No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT4670.24174220031990723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4570.23656570780770367No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT4490.23242451379794077No Hit
TTCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4110.21275384225156718No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC4060.2101655959954654No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG3890.2013655587247193No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA3760.19463611845885465No Hit
TTCCCTACACGACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3370.1744477976612607No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG2970.15374182761244637No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC2650.1371770515733949No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2620.13562410381973383No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT2550.13200055906119132No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG2510.12992996205630988No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT2310.11957697703190273No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC2290.118541678529462No Hit
TCCCTACACGACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.11388283526847878No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2140.11077693976115663No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG2130.11025929050993627No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA2120.10974164125871592No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG2080.10767104425383447No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT2080.10767104425383447No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA2080.10767104425383447No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA2080.10767104425383447No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA1970.10197690249041055No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA1970.10197690249041055No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCA1940.10042395473674948No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)