FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002876657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206
Sequences flagged as poor quality0
Sequence length71
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26221.724709784411278No Hit
ACATATGCCAGTTCCTCGTAGAGATTGGACTGGCTAAGGACGATCAGCTGAAGGTTCATGGGTTTTAAGTG423.482587064676617No Hit
CCTTCCCAGTGGACAGATGAAGAATTAGGTATCCCTCCTGATGATGAAGACTGAAGGTGTAGACTCAGCCT342.8192371475953566No Hit
ATAGAGGGCTGCGTCACTTCTGGGGCCTTCGTGTCCGAGGCCAGCACACCAAGACCACTGGCCGCCGTGGC302.4875621890547266No Hit
GTATGGCTCCAAAGGATGAAATTTCATTCTGATTTTCTGGCTGAAGACTATTCTCTTTGTGTATGTCCACC302.4875621890547266No Hit
CCCAAGGACAGGACTTGCGACCTTGAAGCCAAAGGAATCTCACTTGTGGGGCCTCCTTGTCAGCTCTGCTG292.404643449419569No Hit
GGATTTCCTTGCAATGAGTAGAATTTCCCTTCTCTCCCTTGTCACAGGTTTAAAAACCTCACAGCTTGTAT282.321724709784411No Hit
CATTTCTGTAGCATGTCAGTTTGGAGAAATGATGAGCTACGCCTTGATGAAAGAACCGTGTTGGTGCTGCT241.9900497512437811No Hit
GGCTCATCCCAGCCCAGGTGGGCCAATTATTCAATTTTCAAGAATTTTGTTGCAAGCCAGTTGTCAAACAC211.7412935323383085No Hit
CCTATATCCCTCCCAAAGGGGAGACAAAAAAGAAGTTCAAGGATCCCAATGCACCCAAGAGGCCTCCTTCG191.5754560530679935No Hit
GGTTGGATGGCAAGCATGTGGTGTTTGGCAAAGTGAAAGAAGGCATGAATATTGTGGAGGCCATGGAGCGC191.5754560530679935No Hit
GGTGGATGTTTACCGTGTGTTATATAACTTCCTGGCTCCTTCACTGAACATGCCTAGTCCAACATTTTTTC181.4925373134328357No Hit
GATACTCACTGTTCATGAATACACTTGATGTTCAAGTATTAAGACCTATGCAATATTTTTTACTTTTCTAA181.4925373134328357No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTT171.4096185737976783No Hit
GTTTACTAGTCAGTGAAACTACCAAAGGCTTTTGTGCTGTAGTAATCCTTTTCCTGTTTAAAACTCTGGGG171.4096185737976783No Hit
TACCATGACATTGGGTGACATTGTCTTCAAGAGAATCAGCAAGAGAATTTAAACAAGTCTGCATTTCATAT151.2437810945273633No Hit
CCATGGAGTGATGTGGAGCTAGGATTGTGAGTGACCTGCAGGCCATTATCAGTGCCTCATCTGTGCAGAAG151.2437810945273633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141.1608623548922055No Hit
TCATTAAACCTGTAAGAAATACATCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.9950248756218906No Hit
GAAATTTACTACAGTAGTTAGAATTATATCACTTCACTGTTCTCTACTTGCAAGCCTCAAAGAGAGAAAGT120.9950248756218906No Hit
GACATAAATAGATGGAGATTTAGTGGAGACATGTCATTAACAAGAGTACACACTTTCTCTGTGTTTCATTA120.9950248756218906No Hit
ACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAG110.9121061359867331No Hit
TGCGTGCTCTGTGCCCCCACCAGTTTGCTCACCTCTTGGGGCATTTTGTAATGTGTTTTCTCTGTCTTGTA100.8291873963515755No Hit
GTCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.8291873963515755No Hit
CCCTTGTCACAGGTTTAAAAACCTCACAGCTTGTATAATGTAACCATTTGGGGTCCGCTTTTAACTTGGAC100.8291873963515755No Hit
GTTAGGGTCAGCCATTTTTAATGATCTCGGATGACCAAACCAGCCTTCGGAGCGTTCTCTGTCCTACTTCT90.7462686567164178No Hit
GTGTAACTTACAGTTTCCTTTAAAAAAAAAAAAGTAAACCTGGCAGCTATAGAATACACTATGTGCATTTA90.7462686567164178No Hit
ATGTAAGGGTCATTAGACAAATGTCTTGAAGTAGACATGGAATTTATGAATGGTTCTTTATCATTTCTCTT70.5804311774461027No Hit
GCCAACATTGTGTTTTACTAGAAAATTCCAATTCATGACAGTTCAGAGCTTTTCATTTAGTTCATGTAGAC70.5804311774461027No Hit
GTCCCTATATAGCAGGCTGATTGGGCTGCTTCTGGGATGCAAGCATTTGTGAGAATAATTCAGACATGAGC60.4975124378109453No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG50.41459369817578773No Hit
GTTTGAATTTTTTCTGTAGTGCTGTATTATTTTCAATAAATCTGGGACAACAGCAAAAAAAAAAAAAAAAA50.41459369817578773No Hit
CCTTACACCCAAGCAGTAGGTCTGTGCTCTCCCTGTCTCTAGGTCGCTGAGAGAGGTGCTTTTCTTCATAA40.33167495854063017No Hit
CTTGGGATCTTTGACACTTGAAAAATTACACCTGGCAGCTGCGTTTAAGCCTTCCCCCATCGTGTACTGCA40.33167495854063017No Hit
GCCGCAGGTCAGTCGTGAAGAGGGAGCTCTATTGCCACCATGAGTTTCTCCGGCAAGTACCAACTGCAGAG40.33167495854063017No Hit
GTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTA40.33167495854063017No Hit
CCTGAAGCAAGTGAAAAATAATGAAAGAAGTGAAAATGTCCGGTTCCTGCCTTAACTGATGAAATTGCCTT40.33167495854063017No Hit
GTTAGGGTCAGCCATTTTTAATGATCTCGGATGACCAAACCAGCCTTCGGAGCGTTCTCTGTCCTACTTCG40.33167495854063017No Hit
GATGCTTTAGAAAGCAATTTTCTCTCATCAGTTTCGTCCTTATTCAGCCTTACACAAAAAAAGGCATAGTT40.33167495854063017No Hit
GTGTTAAGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.33167495854063017No Hit
CCCTTACCACGCTACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAAAAAAAAAAAAAAAAAAAA30.24875621890547264No Hit
GGCTTCACAAATAAAGTCGTGTGGCAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.24875621890547264No Hit
CCTGCAGTGGAAAGTGTATAGTGTTTTGTAATAAATGGCCTGATGCTAATGTGTAAAAAAAAAAAAAAAAA30.24875621890547264No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.24875621890547264No Hit
ATAGAGGGCTGCGTCACTTCTGGGGCCTTCGTGTCCGAGGCCAGCACACCAAGACCACTGGCCGCCCTGGC30.24875621890547264No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAA20.16583747927031509No Hit
ACCCTTGGCCATAATATGATTTATCTCCCCACTAGCAGAGCCCAACCGAACCCCCTTCGACCTTGCCGAAG20.16583747927031509No Hit
TTGTTATACATACTGTGTACCTAATTATGTATAGCAGTGTAGTCTCAATTATATCTGAAAGTAATTGTGAC20.16583747927031509No Hit
GTGAATTAAGCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16583747927031509No Hit
CAGTTGATCTCGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16583747927031509No Hit
CCCTACACGACGCGGGCTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16583747927031509No Hit
CAGTATACCCTGGCACACTTAATAAATTAAACATTTGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16583747927031509No Hit
GAATAAAGTTAATACCAAACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16583747927031509No Hit
CCTATATCCCTCCCAAAGGGGAGACAAAAAAGAAGTTCAAGGATCCCAATGCACCCAAGAGGCCTCCGTCG20.16583747927031509No Hit
GGTTGGATGGCAAGCATGTGGTGGTTGGCAAAGTGAAAGAAGGCATGAATATTGTGGAGGCCATGGAGCGC20.16583747927031509No Hit
GACAATTGACAAGGAACTTATTGATCATTCTCCGAAAGCAACTCCTGACAATTAAGCATTTTTTTCTCCAA20.16583747927031509No Hit
GTCTTATTCTGGCAGGTTTTGACGTTTTAAATTTTTTAAAGAAATTTTATTCCTTGGACCAAAAGGTTTGG20.16583747927031509No Hit
TATTTCTGTTGATAAACCATTTGGACAGAGTGAGGACGTTTGCCCTGTTATCTCCTAGTGCTAACAATACA20.16583747927031509No Hit
GTGTAACTTACAGTTTCCTTTAAAAAAAAAAAAGTAAACCTGGCAGCTATAGAATACACTATGTGCATTTT20.16583747927031509No Hit
ACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGACCAACCGAACCCCCTTCGCCCTTGCCGAAG20.16583747927031509No Hit
GTGTGGCAGGTGACCATTGGCACACGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAACTGGCA20.16583747927031509No Hit
GGCAAGGAGTCTCAGGCCAAGGATGTCATTGAAGAGTATTTCAAATGCAAGAAATGAAGAAATAAATCTTT20.16583747927031509No Hit
ACCTCAATCACACTACTCCCCATATCTAACAACGTAAAAATAAAATGACAGTTTGAACATACAAAACCCAC20.16583747927031509No Hit
CACCTATCCCCCATTCTCCTCCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTAAAAAAAAAA20.16583747927031509No Hit
ATTCACAAAAAACAATAGCCTCATCATCCCCACCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCT20.16583747927031509No Hit
GTGTAACTTACAGTTTCCTTTAAAAAAAAAAAAGTAAACCTGGCAGCTATAGATTACACTATGTGCATTTT20.16583747927031509No Hit
TAATATTACTCTGCTACAAAGTGTCTCCAACTAGAAATGATCAATGAAGTGAGAAATTGTTGAGAAGGATA20.16583747927031509No Hit
GGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAA20.16583747927031509No Hit
ATCAAACTATGGGCCAAACGCCACAACATCTATTCCAATATATTAGGTTTCCTCGGTGGTGTTTCCTGGGC20.16583747927031509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)