FastQCFastQC Report
Tue 7 May 2024
EGAF00002876658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110
Total Bases220.8 kbp
Sequences flagged as poor quality0
Sequence length71
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77925.04823151125402No Hit
GAGCACTTTGATATAATGCCTCATTCGGAACTTCACCTGGCAGAAGTTCA561.8006430868167203No Hit
GGGTGAGTAAGTAGTTGGGTATAAGATCTGAGCTTTTCATCTGCAGAGGC481.5434083601286173No Hit
CTTCTGAGCCCAGCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTT401.2861736334405145No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA361.157556270096463No Hit
GCATATGAACTGTACACAGAAGCCCTGGGGATAGACCCCAACAATATAAA341.0932475884244373No Hit
GGCTGAAGCGCAAAAGAAGAAAGATGAGGCAGAGGTCCAAGTAAACCGCT331.0610932475884245No Hit
CACTAGGCATAAATGGTTTATTCAGTTGTGCAAATGAAAGCCATCTGACA300.964630225080386No Hit
GCTCCTCGGGAGGCTGAGGCAGGAGAATCACTTGAACCCAGGAGGCAGAG280.9003215434083601No Hit
GGGCTGACAGCACAGTAACGTGCATACATATCTGCAGGGCTGGAATGGAA260.8360128617363344No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA260.8360128617363344No Hit
GCCCTTGGGCCACGCTCTTGAGGAAGCCCAGGCTCGGAGGACCCTGGAAA250.8038585209003215No Hit
GCTTGGAGGCGAGCATTTTCACTGCTAGGACAAGCTCAGCTGTTGAGGAC240.7717041800643086No Hit
GTGGCTGGAACTTAGAAATGGTTGCCTCTGCTGTTCAGTGAAGTGAGGAA230.7395498392282959No Hit
GCTTAAACAGGAAACCCTTCTTGTTCAGGACTGTCATAGCCACAGTTTGC230.7395498392282959No Hit
CACAGTTAAGGAAGCTGTGCTGAATGGTTTGGTGGCCACTGAGGTGTTGA230.7395498392282959No Hit
AACTAAAGGACTTTGGATGCCACTGGGGAAAGAAGTCAAAGTTATGCAGT220.707395498392283No Hit
CGCCTGAGCGTGGAGAGCACGAGTTAGTTGTAGTCCGGCTTGCGGTGGGG190.6109324758842444No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.6109324758842444No Hit
CAGTAGGGAAATATTGGAAACAACCTAAATGTCCATTTATAAAATGAATA190.6109324758842444No Hit
GAAGCCTGGGTCTCCTCTAGCTTATTTGGTGTCTCCAGAGCTGTCCAGGA180.5787781350482315No Hit
GGCTTATGTTTGGTGGCATTAAATTGGTTTCTTTAAAATGCTTTGGTGGC180.5787781350482315No Hit
AGCCTTGGTGACAGAGCAAGACCCTGGCAAACACACACACACACACACAC170.5466237942122186No Hit
GCAGCTGCTGATGTCTGGAGGCTGATTTTCCTGTTCTCTGTTCTCCACTG170.5466237942122186No Hit
CCTTTGCATCCCTGGCCAGTGACCTTGTATTTATGAGGCCTCTGTGAGGC160.5144694533762058No Hit
GGCGGATCACGAGGTCAGGAAATCGAGACCATCCTGGCTAACACGGTGAA160.5144694533762058No Hit
GCTGGGCTGCTGTGCAGAGTATAAGATTTTGGACTTGTTCATGTCCCCTC160.5144694533762058No Hit
ACACCATGAAGAAGAAATCGTTCATCATAAGAAGGAGATTGAGCGTCTGC160.5144694533762058No Hit
GTAGTAGCGGTGGTCAGACATGGAAATGGTGGGGAGACAAAAATATACAT150.482315112540193No Hit
CACCTGAAGTGCCAGCATTTGGACTTTTGCACCTTTTTTTCCCTTGGCCC150.482315112540193No Hit
GTTGTAGTGAATTGCTACTGAAAGCTATCCCAGGTGATACAGAGCTCTTT150.482315112540193No Hit
CAGTTTACTGCACAGGTGCATCACTTCATGGAGTTATGTTGGGATAAATG140.45016077170418006No Hit
GACCTCGTCCATCTGCACCAGGCTCTGCCTTCACTCCCCCAAGTCTTTGG140.45016077170418006No Hit
GTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACAC130.4180064308681672No Hit
ACAGAGCTCTTTGTAAACCGCAGTCACACATTAGGGTTAGTATTAAACTT130.4180064308681672No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.3858520900321543No Hit
GCTTTCTCTCCAGCCTCTCTTAGGAGGGGTAATGGTGGAGTTGGCATCTT120.3858520900321543No Hit
GGGCCTTCTATGCTGAGCTTTATCATATCATCAGCAGCAACCTGGAGAAA120.3858520900321543No Hit
CCTCCGTCTCTGTGCAGCTCCGTCATTACCATAGGGGACTTGGTTTTAGA120.3858520900321543No Hit
GACCACAATCCACTCCATTCATAAGAGCAAGTCCACTTTCCAGAGTCACA120.3858520900321543No Hit
CCCCACCTCTGTGTGTGATGTAGCTTTCTCTCCCTCAGCCTGCAAGGGTC110.3536977491961415No Hit
GGATCCAGTGTATAAATCCAATATCATGTCTTGTGCATAATTCTTCCAAA110.3536977491961415No Hit
GCCTTGTCTGAAGCTATGGGTCTCTTTTGTTTGATGGTTGCTTTCTTGAT100.3215434083601286No Hit
CCTTTTCCTACCCAAGGGGGAAGACTGGAGCCTAAGCTGCCTGCTACTGG100.3215434083601286No Hit
GCATTTATTAGTATTCTAATAAAAGCATTTTGATCGGAAGAAAAAAAAAA100.3215434083601286No Hit
ATATCTGGCTGTCCTTTTTATAATGCAGAGTGAGAACTTTCCCTACCGTG100.3215434083601286No Hit
CCAATGGCACCTTGTTAAAAGCTGCTATGCCCCAAGAGAAGCATACTACA100.3215434083601286No Hit
ATGAAGAAATTGTGGCACATGATGGCTCCGCTACTTACTTGAGATTCATT100.3215434083601286No Hit
AAATAAATGTATTAATAAAGTTGCATAAAACACAAAAAAAAAAAAAAAAA90.28938906752411575No Hit
GCTTTACTATAGCCGGACAATGGCGAAGTCGTCAGTAGACCAAAAGGATG90.28938906752411575No Hit
GATATAAGAGAAGAAGCAGAGAAATATGCTAAGGAATCTCTGAAGGAAGA90.28938906752411575No Hit
GGGCGTGGTGGCACATGCCTGTAATCGCAGCTACTCGGGAGGCTGAAGTA90.28938906752411575No Hit
CCTTAGGCGATTCACCCACCTCAGTCTCCCAAAGTGCTGGGATTACAGTT90.28938906752411575No Hit
CCCCTCCTGTGTGTGCCCATGGCCCTTTTCTCCTGACCCCATTTTAATTT90.28938906752411575No Hit
CATCGAGAAGGACAAAATCACCACCAGGACACTGAAGGCCCGAATGGACT80.2572347266881029No Hit
CCCTCGGGCCCTCACGGAGGCGGGGGTTCCAGGGCACGAGTTCGAGGCCA80.2572347266881029No Hit
CTTCTAGTAAGCCTCTACCTGCACGACAACACATAAAAAAAAAAAAAAAA80.2572347266881029No Hit
TACCCTATTTTCAAAGGGTAATCGTAAGATGTTAACCCTTGGTATTTAGA80.2572347266881029No Hit
GCAACCATGTAATTATAAAGGAGCCCTACCTCTCAAGAGTGAAGTAAGGG80.2572347266881029No Hit
CCCTCACTGCTGGGGAGTCCCTGCCACACTCAGTCCCCCACCACACTGAA70.22508038585209003No Hit
ATGTTAAGTAACACACTTATTTTTGCCTGTGTATGGAGAGATTCAAGAAA70.22508038585209003No Hit
GTTTTGTTATATAATTTAGACTTGGATTCTTTCCATTTGCATTTGTTTAT70.22508038585209003No Hit
TTTTCCCATCATGGGCCCTGAAGAGGAGGGAGACTCTGCTGCTATAACTG70.22508038585209003No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCCAAAAATCTTTGAAATA70.22508038585209003No Hit
GAAGACAGAATTCAAAGCAAATGCTGAGAAGACTTCTGGCAGCAACGTAA70.22508038585209003No Hit
AGCCAGAAGCATCCCATGGACCATGGGGTGAGTGTCCTCCAAGAGCCCCC70.22508038585209003No Hit
GTCCAGGGCGCTGCGCCTGACGCCTTCGTCATACCCAAATTACGGCAGCT70.22508038585209003No Hit
GGGAAGAAATAAATCTTTGGCTCACAAAAAAAAAAAAAAAAAAAAAAAAA70.22508038585209003No Hit
AAATTAATGTAATAAAGTGAACTTTGTTTTAAAAAAAAAAAAAAAAAAAA70.22508038585209003No Hit
ATTCCATCCTGCCCAACCCTTCCTCTCCCATCCTCAAAAAAGGGCCATTT70.22508038585209003No Hit
ACAGGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCT70.22508038585209003No Hit
TCTCCATACTGTTTCTTTCTTTTTTTTTCATTACTGTGTTCAATTATCTT70.22508038585209003No Hit
GGGGCGGCCTGCCCTCATCTCATTTCTATTAAACGCCTTTGCCAGCTAAG70.22508038585209003No Hit
ATTAAAAAGCGTCACCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.19292604501607716No Hit
GTTGTAAATAGTGTACGATACTAGCATCTGGTATTTTATTTATTTTGCAG60.19292604501607716No Hit
ACAATATATTCTCGGTCCAAGTGAGTAAGTTCTTTGCTTTATGTGAATCC60.19292604501607716No Hit
GCCCTGGGGGCAGCTCAGGCTACTGCTAAGGCCTGAGAGTTTTTGCAGAA60.19292604501607716No Hit
CAATGTATCAGAAGCTTTGAGATCAGCAGGGCTACCATCTCACTCTTCTG60.19292604501607716No Hit
CCTTTCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.19292604501607716No Hit
TGTTTTGGTTATTCATGATCACTTGGGCAGTATAAGAAAATGTAGCTTCT60.19292604501607716No Hit
GTGTTCGCTCTAAACAAAACAGTGGTAGGTTAATGTGTTCAGAAGTCGCT60.19292604501607716No Hit
GACTTGATAAGAGGCGCAAAAGAAAAGAATCTCAAAGTGAAAGGACCAGT60.19292604501607716No Hit
GACCATGGGGTGAGTGTCCTCCAAGAGCCCCCTGAGCTCAGCCCTCTGCC60.19292604501607716No Hit
CTTCAATACAAAGTGTTAATATGTGTCATCAGTATAATAACAACCAACAA60.19292604501607716No Hit
GCTCTGAGCTGCACTGGCCCCGGTGCTGGCATCTGGTGGAGCGGACCCAC60.19292604501607716No Hit
GCCTATAATCCCAGCACTTTGGGAGGACAAGGCAGGAGGATCACTTGAGC50.1607717041800643No Hit
ACTTGTCAAGGAATGTTATCAGTATTAAGCTAATGGTCCTGTTATGTCAT50.1607717041800643No Hit
GAGGTCATTTGATCTTTTGGTAAAGAATCTAAATGGGAAGAGTTACTCCA50.1607717041800643No Hit
GAGATGGTCTGAGTTTGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1607717041800643No Hit
GGACCACTGAAGAAAGACCGAATTGCAAAGGAAGAAGGAGCTTAATGCCA50.1607717041800643No Hit
GAATGAATTATATTTTCAGAAGTAAAGCAAAGAAGCTATAACATGTTATG50.1607717041800643No Hit
CAGAAATGAAACTGTCCTTTTGACAACTCTCTTATATAATAAAGTATCAC50.1607717041800643No Hit
TGTTTATACTGTATGATAATTAAAACATCATGTAAAGATTAAAAAAAAAA50.1607717041800643No Hit
ATGTACCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1607717041800643No Hit
CCCCTAGTGTGCCCAAGTCAGCCTCATATTCTGGGCGGACAGCTTGTCTG40.12861736334405144No Hit
GCAGAGGAGAAGAAAGATGAGAAGAAGGAGGAGTCTGAAGAGTCAGATGA40.12861736334405144No Hit
GATTTATTGCACTTGCAACAGAGTTTAAATAAGTCCTGGGTGTCTGGTGC40.12861736334405144No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12861736334405144No Hit
GCATTACTCTGCACTATAGCCATTTGCCCCAACTTAAGTTTAGAAATTAC40.12861736334405144No Hit
ATGTCCATTCCGCTGTGTTGATTGAGGACGTTCCCTTCACGGAGAAAGAT40.12861736334405144No Hit
ATTTGGGCCTTTTGTATTGCACTCTTGATATTAAATTAAATGTGCCTTGA40.12861736334405144No Hit
GTGTATGGAGAGATTCAAGAAATAATTTTAAAACCGCATACATAATAAAA40.12861736334405144No Hit
GCCTTGTCCAACAGGAAGATATCACAAACCTAAACATATATGCACCTAAT40.12861736334405144No Hit
TGAGGATACAGCAAGAGGGCAGCCAACTACATTCCTGGAAGACAGCCCTG40.12861736334405144No Hit
CAACTGGGTCGGATCATGCTAAAAGGAGATAATATTACTCTGCTACAAAG40.12861736334405144No Hit
GACCTGATTATACAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12861736334405144No Hit
GTAAGATAAGATTTTGACTCTTGTGTTAATGTGTTAGCAAATTAAAAGTT40.12861736334405144No Hit
GAATAAAATTTACCCATTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12861736334405144No Hit
GTATTAAACTTTGTTTAGATGTACCATAAAAAAAAAAAAAAAAAAAAAAA40.12861736334405144No Hit
CTGGAAGAGATTACCAAGAGAATAAAAAGAGACTCATTCAGTGGAAAAAA40.12861736334405144No Hit
ATCCTAACCCTTGAATGACTCAAATCAGTGCCAGGTGGAGGACTCCCATC40.12861736334405144No Hit
ACCTCTTGTATGAGAAACGGTTTGGCCCTTACTACACTGAGCCAGTCATT40.12861736334405144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)