FastQCFastQC Report
Wed 8 May 2024
EGAF00002876787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123553
Total Bases8.1 Mbp
Sequences flagged as poor quality0
Sequence length66
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46043.7263360663035296No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6660.5390399261855237No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGC6580.532564972117229No Hit
TTCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGG2970.24038266978543618No Hit
TTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGAGAGGCGAGG2610.2112453764781106No Hit
TTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAG1950.15782700541468034No Hit
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAACCTGGTC1460.11816791174637604No Hit
TCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAACCTGGTCCAC1430.11573980397076558No Hit
CCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCC1390.1125023269366183No Hit
GCATTACCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG1320.10683674212686055Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)