FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002876820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13819
Sequences flagged as poor quality0
Sequence length71
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175612.70714234025617No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG500.36182068167016423No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG370.26774730443592154No Hit
CTGGAGGACCTGTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAGAGGGAGCTTTTTAAGCTCAAGCAA280.202619581735292No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT240.17367392720167885No Hit
CTCCGGACCCAGCATGTAGGTGCCGGGCGACTGCCATGAACTCCGGAGCCATGAGGATCCACAGTAAAGGA230.16643751356827557No Hit
GTACAAACTTAGAAGAAAATTGGAAGATAGAAACAAGATAGAAAATGAAAATATTGTCAAGAGTTTCAGAT220.15920109993487228No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAA210.151964686301469No Hit
ATTCACAGCCGTGCACGCGCTCAAGCCACCAGGGTGCGAGATGCCAGCTCTGGAGTTCTCGGTTGTTGTAG200.1447282726680657No Hit
GTGTTACCATGGCAAAACTGGAAGAGTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGTAAACAAAC200.1447282726680657No Hit
GTTCATTATAATCTCAAAGGAGAAAAAAAACCTTGTAAAAAAAGCAAAAATGACAACAGAAAAACAATCTT190.1374918590346624No Hit
CCTTCGTCGAGAGTGCCCTTCTGATGAATGTGGTGCTGGGGTGTTTATGGCAAGTCACTTTGACAGACATT170.12301903176785585No Hit
TCCCTGGATTATGCAAAGAAAAATGAACCCAAACATAGACTTGCAAGACATGGCCTGTATGAGAAGAAAAA170.12301903176785585No Hit
GTAAATTGAGATTTAATTCCCAAATGTATTCTACTTGTTCTAAAACAATCTGTCCACAAATATAAAACTAT170.12301903176785585No Hit
CCATGATGGACGATCTTGCTCCCAGGACCTGCCTCTTCCCAGGCTTCCTGGGGAAGAGTTGTACGCCCAGG170.12301903176785585No Hit
CCTGATGTTTTAGAAGTGTATAGTTTAATTTTCAATTATATGTGTTTTCTAGATATCTTTTTTGTCAAGTT160.11578261813445256No Hit
CCTCAGGGACAAAGAGATCTGGACAGAATCGCCGGACAGGTGGCAGCTGCCAACAAGAAGCATTAGAACAA160.11578261813445256No Hit
ATACAGGCAAGAAGACAAAAAGGAGAGAAAATGTACCTCTTCTACTGGAATAATGTTTATGATTACAAGTG160.11578261813445256No Hit
ACACAATGGGGCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCAT160.11578261813445256No Hit
GTATTATATAACATATCTTGAGCATTCTTGACAGACTCCTGGCTGTCAGTTTCTCAGTGGCAACTATTTAC160.11578261813445256No Hit
CTGTATGTATCAATAGTTTATTCTTTTTAACTGTTGAGTATTCCATGGTATGGAAGTACCACAGTTTGTTT160.11578261813445256No Hit
CTATCATTCTAAAGTCATGGACTTCACTTTCGGCAACAAAACTAAATAAGGATGGAACACTTATTGAATGA150.10854620450104929No Hit
GTAATTGAAAGACGTCATCACTGAAAGACAGAATGCCATCTGGGCATACAAATAAGAAGTTTGTCACAGCA150.10854620450104929No Hit
GTGACACCCCTGCCCATTTTCTGTCTTTAATTAACCAAGGTGTTAGGTGTGACTGTCACAACTGTTATGTT150.10854620450104929No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACAACATAAA150.10854620450104929No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAG150.10854620450104929No Hit
GTCCAACAAAGTCCACCAATATGATGGGTCACCGGGTTGATTATTTTATTGCTTCAAAAGCAGTGGACTGT150.10854620450104929No Hit
GTGCCACCTGGTGTCGACAAGAAAGCCGAGGCTGGGGCTGGGTCAGCAACCGAATTCCAGTTTAGAGGCGG150.10854620450104929No Hit
CCTAAAAATTGTATACCGCAGATTCAGGCATGGATTCCGTGAAGGAACAACACCTAAACCCAAGAGGGCAG150.10854620450104929No Hit
GGCCCGGTGCACCATGCATTGCAATGACAAAGCCAAAGATTCAATAGATGCTGGGAGTAAGGAGCTTCAGG150.10854620450104929No Hit
GCATTATTGAGCCTTCTCTCCGCCAGCTTGCCCAGAAATACAACTGCGACAAGATGATCTGCCGCAAGTGC140.101309790867646No Hit
GTATTTATCTGGAGACCGGGTCTCACTCTGTCACCCAGGCTGTAGTGCAGTGGCACGATCTTGGGTCACTG140.101309790867646No Hit
GAAAAAGAAAGAAGCCAAAGAGAAAGGTACCTGGGTTCAACTAAAGCGCCAGCCTGCTCCACCCAGAGAAG140.101309790867646No Hit
TATTGAAACGGTGTGAAGACGGATTCTTTGGTTGATAAATTGCTATCATTCTAAAGTCATGGACTTCACTT140.101309790867646No Hit
CCGTGAAGACCTGGAGCGACTGAAGAAGATTCGGGCCCATAGAGGGCTGCGTCACTTCTGGGGCCTTCGTG140.101309790867646No Hit
GTCCTCGTCCTTGGCATTTTCACTGTTCTGTACAAGGCTGCTTGTTTTTTTATTGCCAAAGTCAAATAAAC140.101309790867646No Hit
GTGCAGGTACCATCAGGAAGCAGCGGGATAAGCTGATAAAGGAAGGAAAGTACACCCCTCCACCTCACCAC140.101309790867646No Hit
GAACTGTCCTTTCTGCGCTCAAATTTTTCGAGTGTGCCTTTTTATTCATCTACTTTATTTTGATGTTTCCT140.101309790867646No Hit
GGTTTAATAGAAATCCTTAAAAAAGTAAGCCAACAAACAGAAAAGACAACAACAGTGAAATTCAACAGAAG140.101309790867646No Hit
ACAGAGGGAAATTACTGTTACTGTGTCAAATATGTGACTGATGCTTTTGGTCAGATAATCTGCTGCCTGTC140.101309790867646No Hit
TGTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCTGTCCCAATGTATGGGATGGCGGATAGTAAG140.101309790867646No Hit
AGCTGGGTGAGATGTGGAATAATTTAAATGACAGTGAAAAGCAGCCTTACATCACTAAGGCGGCAAAGCTG140.101309790867646No Hit
GAAGTACAAGCCCCTGGACCTGCGGCCTAAGAAGACACGTGCCATGCGCCGCCGGCTCAACAAGCACGAGG140.101309790867646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)