FastQCFastQC Report
Wed 8 May 2024
EGAF00002876865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002876865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172886
Total Bases12.2 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36162.091551658318198No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA32861.900674432863274No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA29711.7184734449290282No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT14830.8577906828777345No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC14480.8375461286628182No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGG11040.6385710815219278No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG10390.6009740522656548No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT10370.599817220596231No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG8610.4980160336869382No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC8500.49165345950510736No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA8400.48586930115798854No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA7450.43091979686035886No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTG7430.4297629651909351No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCC6990.40431266846361186No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6780.3921659359346621No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6180.3574609858519487No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTAC5900.3412653424800157No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAA5420.31350138241384495No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGG5230.30251148155431906No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA5190.3001978182154715No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAA5180.29961940238075957No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT5080.29383524403364064No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC4490.2597087097856391No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT4470.2585518781162153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4290.24814039309140126No Hit
AACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTT4080.23599366056245158No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT3910.22616059137234942No Hit
GAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATA3830.22153326469465429No Hit
AGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA3190.18451465127309324No Hit
CTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAAT2970.17178950290943165No Hit
ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAA2400.13881980033085387No Hit
ACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATC2400.13881980033085387No Hit
AATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG2380.13766296866143007No Hit
CTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCT2270.1313003944795993No Hit
GAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAA2240.12956514697546362No Hit
GTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTC2230.1289867311407517No Hit
AATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC2180.12609465196719225No Hit
ATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAA2090.12088890945478523No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCAAAAAAAAAAAA2080.12031049362007334No Hit
ACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCAC2030.11741841444651388No Hit
ACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.116839998611802No Hit
CCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.11163425609939498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)