FastQCFastQC Report
Tue 7 May 2024
EGAF00002877043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14930
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA9436.316141995981246No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2441.634293369055593No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA1931.2926992632283993No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG1220.8171466845277965No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACC1020.6831882116543871No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT740.49564634963161425No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAA710.4755525787006028No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGG690.4621567314132619No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTAC660.4420629604822505No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC650.4353650368385801No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA650.4353650368385801No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG580.3884795713328868No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT530.35498995311453446No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA520.348292029470864No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAA510.3415941058271936No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG500.33489618218352313No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGG470.31480241125251174No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA430.2880107166778299No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCC420.2813127930341594No Hit
GAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATA390.261219022103148No Hit
GGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA370.24782317481580712No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT360.24112525117213662No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.22103148024112526No Hit
ACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAA330.22103148024112526No Hit
ACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATC320.21433355659745476No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.20763563295378434No Hit
ATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAA300.20093770931011384No Hit
CAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAA290.1942397856664434No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC280.18754186202277295No Hit
AACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTT280.18754186202277295No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCA270.18084393837910248No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA260.174146014735432No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC260.174146014735432No Hit
TAATAAAATTATTCACTTGGTAAGTGTTCAAGTCTTTCTGATCACCCCAA250.16744809109176156No Hit
GTACAAACAGATCCTACTGATGAAATTCCCACTAAAAAGTCAAAGAAGCA240.1607501674480911No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTG240.1607501674480911No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCAAAAAAAAAAAAAAAAAAAA240.1607501674480911No Hit
ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAA230.15405224380442062No Hit
GTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTC230.15405224380442062No Hit
TCCCTACACGACGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.14735432016075017No Hit
GCACTAAAAGCACTCTGAGTCAAGATGAGTGGGAAACCATCTCAATAAAC220.14735432016075017No Hit
AATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATAG220.14735432016075017No Hit
AAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT220.14735432016075017No Hit
GCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAAT220.14735432016075017No Hit
TTCCCTACACGACGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1406563965170797No Hit
CCCTAAAAATCTTTGAAATAGGACCCGTATTTACCCTATAGCACCCCCTC200.13395847287340923No Hit
CTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAAT200.13395847287340923No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCAAAAAAAAAAAAAAAAAA200.13395847287340923No Hit
ACCTTCAGGACCTTAATAAAATTATTCACTTGGTAAGTGTTCAAGTCTTT190.12726054922973878No Hit
GACCCGTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAA190.12726054922973878No Hit
GGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTC190.12726054922973878No Hit
GCTATATACTTTGGTTACCTATGTACCTGTTACCACTTTCAAAAATCTAC190.12726054922973878No Hit
GACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCAC180.12056262558606831No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCAAAAAAAAAAA180.12056262558606831No Hit
CTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.12056262558606831No Hit
GTTATTTTCTCATTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.12056262558606831No Hit
TCCCTACACGACGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.12056262558606831No Hit
GGAAAATCAAGGCCCGCAAAGGCAAATAAATCCTTGTTTTGTCTTCACCC160.10716677829872738No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT160.10716677829872738No Hit
TCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTT150.10046885465505692No Hit
ACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10046885465505692No Hit
CCCTACACGACGCGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAG150.10046885465505692No Hit
TGTCTCAAGTAAGCTTAAACTACAACTTGTCACATAAAGGAAGTCTTAAG150.10046885465505692No Hit
ATATAATTTATGTATCACCTTCATTGTAAATTTAGCAGGAAATGCATCAC150.10046885465505692No Hit
GGGAAGAGATGATCATTGGAAGCAATCAGTTTACTCTTCCCCACCACAGT150.10046885465505692No Hit
ATATAAGCCAGACTTGAAAGAGGGCTCCAGAAAAAGTAGATGCGTATCTG150.10046885465505692No Hit
AACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCC150.10046885465505692No Hit
ACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCAC150.10046885465505692No Hit
CCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10046885465505692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)