FastQCFastQC Report
Tue 7 May 2024
EGAF00002877048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77015
Total Bases5.4 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6730.8738557423878465No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA4690.6089722781276374No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT2380.3090307083035772No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2360.30643381159514377No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC2130.27656949944815945No Hit
GGCTTGAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC1570.20385639161202362Illumina Single End PCR Primer 1 (100% over 40bp)
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT1540.1999610465493735No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA1390.18048432123612285No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA1310.17009673440238915No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAA1210.15711225086022204No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACC970.12594949035902098No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC890.11556190352528728No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA890.11556190352528728No Hit
GCTTGAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG870.11296500681685386Illumina Single End PCR Primer 1 (100% over 41bp)
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT860.11166655846263716No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGC860.11166655846263716No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAA840.10906966175420373No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTAC830.10777121339998702No Hit
TTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAG810.10517431669155361No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC790.10257741998312016No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCA780.10127897162890347No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)