FastQCFastQC Report
Wed 8 May 2024
EGAF00002877054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018448
Total Bases72.3 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130441.2807723123811918No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA54910.5391536926774857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16960.16652789342214822No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC15380.15101409203022637No Hit
GTATTAATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC14430.14168617347179238Illumina Single End PCR Primer 1 (100% over 40bp)
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA13460.1321618776805492No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC12630.12401222251897004No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT11910.1169426421378411No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT11010.10810566666142996No Hit
GTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAGCTTTC10840.10643646018255228No Hit
TTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAG10340.10152702936232386No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGC10300.10113427489670558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)