FastQCFastQC Report
Wed 8 May 2024
EGAF00002877056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262114
Total Bases160.6 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA182730.8077842230762905No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169750.7504042678662526No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162360.7177357109323402No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA62050.27430094150869494No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGC39270.1735986780507083No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC37600.16621620307376198No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC36400.16091143063523766No Hit
TTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGACCCTCAGGGTAGGCGAG34150.15096498231300456No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT30090.1330171688959973No Hit
GGGTAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAACCTGGTCCACAT29720.1313815307274523No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACC29300.12952486037396876No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA28790.12727033208759592No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT28080.12413167506146905No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAA23810.10525552646772002No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAA23020.10176321794569151No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG22780.10070226345798665No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)