FastQCFastQC Report
Wed 8 May 2024
EGAF00002877099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893986
Total Bases63.4 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107391.2012492365652259No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA70750.7913994178879759No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27670.30951267693230095No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT20780.2324421187803836No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC16250.18177018432055983No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC16010.17908557852136386No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA15370.17192662972350797No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT14370.16074077222685815No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA11880.13288798706020005No Hit
CTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCA10850.1213665538386507No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC10470.11711592798992379No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA10200.11409574646582832No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA9540.10671308051803943No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACC9220.10313360611911147No Hit
GTCCATAGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC9030.10100829319474802Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)