FastQCFastQC Report
Wed 8 May 2024
EGAF00002877105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094190
Total Bases77.6 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACC159651.459070179767682No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC156041.4260777378700227No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA66270.6056534971074493No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63440.5797896160630238No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA59960.5479852676409034No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA58720.5366526837203777No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA42620.3895118763651651No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA36060.3295588517533518No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA35060.3204196711722827No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG33910.3099096135040532No Hit
ACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACCTAAGGGTC26830.245204214990084No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT26520.24237106900995256No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG25860.23633920982644696No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA25030.2287536899441596No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG23460.21440517643188112No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA22940.2096528025297252No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC22450.20517460404500132No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA22420.20490042862756924No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA21440.19594403165812155No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA21180.19356784470704358No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA21140.1932022774838008No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA20500.18735320191191657No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT20330.18579954121313483No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC19770.18068160008773612No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC18640.17035432603112804No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT18630.17026293422531735No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA17910.1636827242069476No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC15890.14522157943318803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14970.13681353329860446No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA14940.13653935788117239No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA14870.13589961524049754No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG14490.1324267266196913No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG14340.1310558495325309No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT13860.12666904285361774No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG13570.1240186804851077No Hit
CCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATA13170.12036300825268008No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC12930.11816960491322348No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA12910.1179868213016021No Hit
GACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAG12370.11305166378782479No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT11770.10756815543918333No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC11470.1048264012648626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)