FastQCFastQC Report
Wed 8 May 2024
EGAF00002877133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217749
Total Bases15.4 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37421.7184923926171876No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6010.27600585995802507No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA5750.264065506615415No Hit
CTACACGACGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT4010.18415698809179376Illumina Single End PCR Primer 1 (100% over 37bp)
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG3500.16073552576590477No Hit
TCCCTACACGACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.12537371009740572No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA2610.11986277778543186No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT2600.11940353342610069No Hit
TCCCTACACGACGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2340.10746318008349061Illumina Single End PCR Primer 1 (100% over 34bp)
TCCCTACACGACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2310.10608544700549714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)