FastQCFastQC Report
Wed 8 May 2024
EGAF00002877138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040448
Total Bases73.8 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130041.2498462200898075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35440.34062250107645936No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA22910.22019360890693238No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG17270.1659861905640647No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA13300.1278295503475426No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT11240.10803038691025404No Hit
GCATTACCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG10970.10543535092575507Illumina Single End PCR Primer 1 (100% over 41bp)
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT10490.10082195361997909No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)