FastQCFastQC Report
Tue 7 May 2024
EGAF00002877194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4920
Total Bases349.3 kbp
Sequences flagged as poor quality0
Sequence length71
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA141028.65853658536585No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA621.2601626016260163No Hit
GTGTTTATGGAGTTTAAGAAATTTATTTTCCAGGTGCAGCCCCTGTCTAA490.9959349593495935No Hit
GGATGTGCATGCATCTTGAAGCAACAAAGCAGCCACAGTTTCAGACAAAT480.975609756097561No Hit
GTTGAATTTTACATGGCTGGAGCTAGAATTTGATATGTGAACAGTTGTGT440.894308943089431No Hit
CCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATA430.8739837398373983No Hit
CAGTTTAGAGGTTTGCAGAGCCGAATTGAACAAGAACAGAAGTTCACTGT400.8130081300813009No Hit
CATGGTGTCCTTTGGACATAAGAGATACCAGCAATGTGTGCACAGAACAA400.8130081300813009No Hit
GAATGTATCTCTCCAAGATGAGAGCTCATTAAAAGATAATTACAAAGCTT350.7113821138211381No Hit
GATGATGAAGCCCCGTCTGTACTAAAAATACAAAAATTAGCCGGACGTGG340.6910569105691057No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT330.6707317073170732No Hit
CCTCTCTACAAATGGCCCTGTAGAAGCTGTGCTCAGTGATCCACTTCCTG330.6707317073170732No Hit
AACTACTGCCAATGTTTAAATTGCCCTTATGTTTATGTTTATTATGTCAA300.6097560975609756No Hit
GCACTCCATGCATTCTGATAACGGCAGCTTTCCTAAAAAGAAAAAGTTAT280.5691056910569106No Hit
GGTAAAGGCAGACCGAGATGAATCCTCACCATATGCTGCTATGTTGGCTG260.5284552845528455No Hit
GCTGCAGTCACTGTACTTAGAGTGGATCAGATCATCATGGCAAAACCAGC260.5284552845528455No Hit
GATTTGATCCCTTTATGAACCTTGTGATAGATGAATGTGTGGAGATGGCG250.5081300813008129No Hit
GTATCATACATAGGAGTATGCCAGAGTAAATTCCATTTTTTTGAAGATCA250.5081300813008129No Hit
GAAATCAAGGACTTCCTGCTCACAGCCCGACGAAAGGATGCCAAATCTGT250.5081300813008129No Hit
GGGTGCCTCATCCCAGCAACCTGTCCTTGTGGGTGATGATCACTGTGCTG250.5081300813008129No Hit
GATTTCATTCAATGTGTCATGAGTGTTTAAATCAAGAGGAAGCTGCATGA220.4471544715447155No Hit
GCCTCGTAGAGCTTCACAGCTACTCCAGAAAATAGGGTTATGTGTGTCAT220.4471544715447155No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220.4471544715447155No Hit
GGTGGAAATAGAAACCGTACATGTCAGTACAGAAAAGCTTAAGAATCGAA220.4471544715447155No Hit
GGAAAAGGGCAAATACAAGGAAGAAACCATTGAGAAGATGCAGGAATAAA200.40650406504065045No Hit
GAATAAACAAATTGAGACAGTGAAAAAGTTGAATGAGTCACTCAAGGAAC200.40650406504065045No Hit
CTATTTGAAACCAAAATACCTGGCCCAAGTTAGGAGGTTTGTTTCAGACC200.40650406504065045No Hit
GTGCATTACATTTGGAAAAAAATGTGAATCAGTCACTACTGGAACTGCAC200.40650406504065045No Hit
GCTTGAGTGAGGATGAAGCACATCTTAAAGGAATGAAGTGTAATGCTTGA200.40650406504065045No Hit
GACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC200.40650406504065045No Hit
GAACCAGGGTGACATCAAGCTCTGTGAGGGTTTCAATGAGGTGCTGAAAC190.3861788617886179No Hit
AAGTAGTTGGTTTGTATGAGATGGTTAAAAAGGCCAAAGATAAAAGGTTT180.3658536585365854No Hit
GATCTCAAATCAGCACCCTAACATCACAATTAAAAGAGCTAGAGAGGCAA180.3658536585365854No Hit
GCTTGGAGAACAGCCTGAGGGAGGTGGAGGCCCGCTACGCCCTACAGATG170.34552845528455284No Hit
GCTACATTTGAAGCCTGTACAAAAGCTTATCCCTGTAACACATGTGCCAT170.34552845528455284No Hit
GCTGCCAAGCATCCTTCAATGAACAAGGCACTCCTCACAATAAAGAATTA170.34552845528455284No Hit
GGTGGTGCTTTTGTGCTTGTCTTGTATGATGAAATCAAGAAGTACACATA170.34552845528455284No Hit
CCTTAATACACTGCTATGGAGGACTTGGGAGATCTTGTCTTGTAGCTGCT170.34552845528455284No Hit
GTTTATTGTTGGACAACATTGTTTCACACATACATCAAACAGGCCAAAAA160.3252032520325203No Hit
GGATGTCACCCCCATCCCCTCTGACAGCACTCGCAGGAAGGGGGGTCGCC160.3252032520325203No Hit
GGAGCGCTTTGGGTCCAGGAATGGCAAGACCAGCAAGAAGATCACCATTG160.3252032520325203No Hit
CATCCAAACTGTATCTTCCTTTACTGTTTATATCTTCACCCTGTAATGGT150.3048780487804878No Hit
GCATCTAAAGTGAAGACTTAAGAAAAACTTAGTGACTACTAGATTATCCT150.3048780487804878No Hit
CTGTGACCTGACCAGCCCCACCGGCCCATCCTGGTCATGTTACTGCATTT150.3048780487804878No Hit
GTTTATTACAACTACTTTTAGGCCTGAACTGCTTGAGTCAGCTGACAAAT150.3048780487804878No Hit
GTCCGTGCTCCTCCAGCTGCCCCAGTGAGGGCCCACCCTGCCTGCACCTC140.2845528455284553No Hit
GTTCCAGATAGTTTCATTTCACCTGTAAACTGTATCACTTTGACTGATAT140.2845528455284553No Hit
AGTGGAGGAGACTTGGTGTCCAACAGAGTCTAGGCTGGGTTCATTACATG140.2845528455284553No Hit
CTGCCCCCATCTCTTCTGATTGCTGTTAACAAATGACTAGCTTTGCACAG140.2845528455284553No Hit
GCCTGTGACCGCGGTTGGCGAGGGCCTGGACCGAGTATGGGCTTATGATG140.2845528455284553No Hit
GCTATAGCCTAAGCGGCTGTTTACTGCTTTTCATTAGCAGTTGCTCACAT140.2845528455284553No Hit
GGGCACATCGTGACTGTGTTTTTCATAACTTATGTTTTTATATGGTTGCA130.26422764227642276No Hit
CTTCTGTAGCTCAGGAGAGCACCCCTCCACCCCATTTGCTCGCAGTATCC130.26422764227642276No Hit
GGCTTCATCTCACTTGGAAACATTGACCTCAGAAGTTACACTGACATAGC130.26422764227642276No Hit
GCCTCTGAGCAAGACAGTGCGCTTCAACGTGCTCAAGGTCACCAAGGCTG130.26422764227642276No Hit
CTTTGACTTCTTGCATGCGAGGGACCATTGCGTGGCCCACAAACTCTTTA130.26422764227642276No Hit
TGTGTACCTTTTTATCTTTTGCTTTGCTTTATTGTTATCAATCAGATAAA130.26422764227642276No Hit
GGCCGAGAGGGCCCAAATTGCTTGAGCCCAGGCGTTCAAGACCAGCTTAG130.26422764227642276No Hit
CACATGGGGTTCCTGGTTGTTCCTGCATCCCGATACCTCAGCCCTGGCCC120.24390243902439024No Hit
TCCCTACACGACGCGGGTCAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAA120.24390243902439024No Hit
GCTGAGGAGAGACCAGGTGGACCCCAGCTGCCTGTCACCCTTCATCTGGG110.22357723577235775No Hit
GCCCCGCCTGGGTGGCCGGACCCCTGGGCCTTGGGGCTGTGGATCCTGGT110.22357723577235775No Hit
GGGGTGGGCGGAGAGGAGAAGTATTGGAACTTCTTTCTTCTACCCTCTAG110.22357723577235775No Hit
CAATGTAACCATATGGAAGCAACTTTTTTTGGTGTCTCAAAGGAGTAACT110.22357723577235775No Hit
CATTCCACAGGCCCATGGACTCACTTTTGTAACAAACTCCTACCAACACT110.22357723577235775No Hit
GTAACAAGAGGAAATCCAATTTCTCAAACAGTGCCGATGACATCAAATCT110.22357723577235775No Hit
GCCGGGTGTTCCCAGACAAAGAGGTCATGCTGGATGCTGCCTTAGCGCTG110.22357723577235775No Hit
ACACGACGCGGGCCTGACTGTAATAATATTAAACTTTTTTAAAAAACCAT110.22357723577235775No Hit
GCCTCATAGCTGAAGTTGCCTGAATGATCCTCCTGTTGCATGTAACCCAC100.20325203252032523No Hit
GGAACGACCTGAGGCCCAAGCTCAGCTGCCTCTTTGCGGGACCACACAGC100.20325203252032523No Hit
GCAGTGCTACCGGATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACA100.20325203252032523No Hit
GAGAGTGGCTGGAAGAGACTCCAGGCCCCTGGGGAGACTGTACTGTTCCT100.20325203252032523No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.20325203252032523No Hit
GGGAGTCTGTGTTCCACACAACCCGCTGGGTGCCCCCCTTGATCGGGGAG100.20325203252032523No Hit
GAGGCAAGAGAATCTCTTGAACCTGAGAGGCGGAGGTTGCAGTGAGCCAA90.1829268292682927No Hit
CCACCCTTGCCCCAAGTCTTTTAACCTGGGTGTTAGCATTTTAAAGAGAC90.1829268292682927No Hit
GGATCCTACATGGAGAGAGACAGAACTCTCTCAGCTGACACTCTCAGAGA90.1829268292682927No Hit
GCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCT90.1829268292682927No Hit
GGGGTGTCTGAGGCCAGGGAGAGCTCTACTCATTCCCCTGTGTGATCCCA90.1829268292682927No Hit
CTCCAATAAAGTGCCTTCTGGGCTTTTTCTAACCTTAAAAAAAAAAAAAA80.16260162601626016No Hit
GAAGTGTATTCCTTTTCAGGCCTGCAGAGTGTCCCTCCCTTGGCTCCAGA80.16260162601626016No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.16260162601626016No Hit
CAAGAAGAAAGTGTTCAAATTAAAAAAGCTGCTGCCAAGTAAAAAAAAAA80.16260162601626016No Hit
GGATCCTGGATGTGTGTGTTCTTGTTAAAGAGGCTGTTATCAGGCTTAAC80.16260162601626016No Hit
GAGGCAGAGGTTGCTGGGAGCCAAGATTGTGCCACTTAACTCCAGTCTGG80.16260162601626016No Hit
GAACGTAACCTGAAGTAACCTGATGTTAACCAATCTGCTGTGTCTACTAT80.16260162601626016No Hit
CTGATTTTACGTAGATCATCCTTACAGTCCAACACAGGAAATATTTGTTT80.16260162601626016No Hit
CACTCCGCGCCGATGCCACCGGCCTGTGGGTCTCTGAAGGGACCCCCCCC80.16260162601626016No Hit
GCTTGAAGCCACATGGAGGGAGTTTCATTAAATGCTAACTACTTTTAATA80.16260162601626016No Hit
CTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGG80.16260162601626016No Hit
GCACCCTAAGCATCCTGCTGCCTTCCCACAACATTAAACTCTCCTTCCTC80.16260162601626016No Hit
GGGCAGGGGGCAGCAGGATACCTCCAGCCAGGCCTCTGTGGCCTCTGTTT70.14227642276422764No Hit
GTATTTAAGGTTTCCAATAAAATGTACACCCCTCAAAAAAAAAAAAAAAA70.14227642276422764No Hit
GGCCGTACCACTGTGGCTGCTGGCAGCATCTCTGCTCTGCCTTCTCAGCA70.14227642276422764No Hit
CTGCAAGATGGACACGAGCCACAAGCTGCACTGTGAACCTGGGCACTCCG70.14227642276422764No Hit
GAGCAACCCACCAAACCTAACAAGGACCCCCGAACACTTTCATACTAAGA70.14227642276422764No Hit
CTTTCCCTACACGACGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.14227642276422764No Hit
GGAGAAATTGTGACAACAATTTGAAAATTGTGGTTGCATTTTAAAGTGTG70.14227642276422764No Hit
CGCCTGAACCCAGGAGGCGGAGGTTGCAGTGAGCTGAGATCGCGCCACTG70.14227642276422764No Hit
GGTCTAGAAAACCAGCTTGCGAATAACAGTCCCCGTGGCCATCCCTGTGA70.14227642276422764No Hit
GTTACTCGGGAGGCTGAGGCAGGAGAATTGCTTAAATCTGGGAAGAGGAG70.14227642276422764No Hit
TGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAAAAAAA70.14227642276422764No Hit
GCCTCGTGTCATCTCCGGGGTGGCCACAGGCTAGATCCCCGGTGGTTTTG70.14227642276422764No Hit
GTAAAAACCCCAGGCACTCAGTCTCTAACAAGCTTTTCTGGTTGACAGTG70.14227642276422764No Hit
ATTGTATGTAGCTCGTGATCACATTTTCTGTTGGTTAACATTGTTCCTAG60.12195121951219512No Hit
CTGTTATGTTATGATTTTATTCTTTGTGAGTTAATTAGAATAAAGTCATT60.12195121951219512No Hit
AGATTTCCTGTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12195121951219512No Hit
TGTATTTTACGTACAAGGTTCGCTACACTAACAGCTCCACCGAGATTCCT60.12195121951219512No Hit
CTCCTACGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12195121951219512No Hit
CATCAACGAGGGGAAGCTGTGGAAACACATCAAGCACAAGTATGAGAACA60.12195121951219512No Hit
GGCGTGAGCCACCACACCTGGCCTCAAGAGAGCCACCTACCTCGGCCTCC60.12195121951219512No Hit
CTCTTGATGTCTTAGTAAATTTGCATTTGATATATCATTGATGAGATTTT60.12195121951219512No Hit
GGCTTATTAGAAGAATGAACTAAGGTGTCTACCATGATTATTTTTCTAAG60.12195121951219512No Hit
GAATTCCAGTGCCTCCTACGGGGGCAGCACACTTAGATATTCCTGGACAT50.10162601626016261No Hit
GTGTTCGCCCTCCTGCGAGGAGTAGAGTGTGGTGGGTCCGAGCTAGTGAA50.10162601626016261No Hit
CCTGGGAGGCGGTGGTTGCAATGATCTGAGATCATGACACTGCACTCCAG50.10162601626016261No Hit
CCCTGGGAGTGGGTGGAGGCAGCCAGGGCTTACCTGTACACTGACTTGAG50.10162601626016261No Hit
TTGCTGACTGTAGCTTTGGAAGTTTAGCTCTGAGAACCGTAGATGATTTC50.10162601626016261No Hit
CCTGCGCAGCCTGGCCTGCCAGCCCATCAGCTTCTCGGGCTTCGGGGCAG50.10162601626016261No Hit
CCTGGGCAACTTCGCCAAGGCCACCTTTGATGCCATTTCTAAGACCTACA50.10162601626016261No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA50.10162601626016261No Hit
TCCCTACACGACGCGGGAGGAAAAAAATCAATAAACAGTTGATCTCGTGC50.10162601626016261No Hit
CTTGAATACCTGGCTAAGGAGTTTAAAGTGTATCCAAGAGGACGTGGGAG50.10162601626016261No Hit
GATAATTAAAAAGAAAACCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10162601626016261No Hit
GTTGTATCCTTTAATTTCCAGCCTTTTAGGTTAGTTTCTGTAACAGAACA50.10162601626016261No Hit
TTCCGCCCCATTCTGTCCCCCTCATGGCCACCTTGCCATGTCCGCGCCGG50.10162601626016261No Hit
CAGATGCCTCCCCACCACCAATTGCCAAAGGTCCAATAAAATGCCTCAAC50.10162601626016261No Hit
CCTACAAAATAGTTATACATAGTGGAATAAAGAAGGTAAACCATCAAAAA50.10162601626016261No Hit
GGACAGGGGGTTTCTGTTGCAAAAATACAAAACAAAAGCGATAAAATAAA50.10162601626016261No Hit
GGCGTGAGCTACCACACCTGGCCACATGATTCTATATCTGGAAAACCCCA50.10162601626016261No Hit
TCTTGGTAGTGCCTCAGATCCTAATTTTGAATATGGGTTGAGTGAGATAA50.10162601626016261No Hit
GGATCTCTACCAGCAATGTGGAATTATCACCCATCATCATCCAATCGCAG50.10162601626016261No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)