FastQCFastQC Report
Tue 7 May 2024
EGAF00002877210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7068
Total Bases501.8 kbp
Sequences flagged as poor quality0
Sequence length71
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA178525.254668930390494No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGA470.6649688737973968No Hit
GGCTCACATCTGTAATCCCAGCACTTTGGTAGGCCGAGGTGGGTGGATCA430.608375778155065No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA420.5942275042444821No Hit
CCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA400.5659309564233164No Hit
GTTCTCGGCTCCCACAGCAGAGCCAGGTGAGGGGGGGCCTGCCAGGACTA380.5376344086021506No Hit
GGCTTTGTAAATTTAAATATGTAAGTGCTATATAAGTCAGGAGCAGCTGT380.5376344086021506No Hit
CATGGTGAAACCCATCTCTACTAAAGATACAAAAAAATTTGGCCAACTGT350.4951895868704018No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340.48104131295981895No Hit
GGCCTCGAGCAGGTAGGACCCGGGCGATAGCCAGGACACGGCAGAGTGAG340.48104131295981895No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC330.46689303904923596No Hit
ATTTGATTAATCTGGTCAATGAGAATCAAATGGAAACATATCATTCTGGA300.4244482173174873No Hit
CCAAGGCAGTGCATGCATGTTGGGGTTTCCTTTACCTTTTCTATAAGTTG290.41029994340690434No Hit
GATGACTGTCAGGATGTTGCTTCAGAGTGTGAAGTCAAATGCATGCCAAC290.41029994340690434No Hit
GACCGAGAGTCTGTCTCAAAAAAAAAAAAAAGTTGAACAATACCAAACCA290.41029994340690434No Hit
GAAGTTGGAATGTTGGAGATGCAAGAATTTCCAAGAACTTTCTTAGCCTT280.39615166949632147No Hit
GGGGTTTTGTGTGTAGGTTTATGAAGAAGATTGGCTGGCAGATTTCCTTT280.39615166949632147No Hit
GATACAGAATCTGTATTGTTCTTACTGAAACACAGCATGGAATTAACATT270.38200339558573854No Hit
GATTTCCTGCCCAATATTTGTCTTTGGGCTGTCTCTAGTGACTAATTATT270.38200339558573854No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG260.36785512167515566No Hit
GTTTGATACCAGCCTGAGCAACATAGTGAGACCCTATCTCTACAAAATTA260.36785512167515566No Hit
GTGATTGGGGGAGGGGACAGGATCTAACAATGTCACTTGGGCCAGAATGG260.36785512167515566No Hit
GCTTATAGCTTTAAGTGCCTACATCAAAAAAGAGGAAAAACTTTAAATAA260.36785512167515566No Hit
GTGAACTCTGGCTTTTTATCCTCTGAGAAAACAGCCCACAGGACTGGGTC260.36785512167515566No Hit
ACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCAC250.35370684776457273No Hit
GTGGAAGAACGGTCTCAGAACTGTTTGTTTCAATTGGCCATTTAAGTTTA250.35370684776457273No Hit
GGGTACAGATGCAAGTCTTGATGAGGAACTTGATCGAGTCAAGATGAGTT240.3395585738539898No Hit
CACCAAACAAAAATGCCATTTTAAAAAAGGTGTATTTTTTCTTTTAGAAT230.3254102999434069No Hit
GGTCATGGCCGCCTGCCTGTCATTCCTGACTCACCACCGTCCCCAGGTGA230.3254102999434069No Hit
GATTAAATATATCTTTGTAAATTATTTTATGTCATAGTTTAATTGGTCTA230.3254102999434069No Hit
GTGAAAATGACCCTGAAGAACAGAGAACCTGTACAGCTGGAAACGCTGAG230.3254102999434069No Hit
CTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCT220.311262026032824No Hit
GGGCGGATTGAGGATGTCACCCCCATCCCCTCTGACAGCACTCGCAGGAA220.311262026032824No Hit
GGCAACATAGGGAGACTCCATCTCTACAAACAATAAGAATAAATTAGTTG220.311262026032824No Hit
CGTCTCTGTGTATGTGAAGTAGGGGAGGCCCTGGGGGATGACCTCCCAGC220.311262026032824No Hit
GCCAGCAAGTGCCGCCGGCCGGCTGTCAAGCAGTTCCACGACTCCAAGAT210.29711375212224106No Hit
GAACATGCCTAGTCCAACATTTTTTCCCAGTGAGTCACATCCTGGGATCC210.29711375212224106No Hit
CCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCAGATCCCTTTAGCC210.29711375212224106No Hit
CGTTAATTCCTGTGGACCAGAACTGATTCCCACAGCTACAGTTCAGCTTG210.29711375212224106No Hit
CGTTTGGCCTCTGACAATACTGGTATAATTGTAAATAATGTCAAACTCCG210.29711375212224106No Hit
GAGTGGAGGAGACTTGGTGTCCAACAGAGTCTAGGCTGGGTTCATTACAT210.29711375212224106No Hit
GGCTGGAGGGCTGGGAGGTGTTTGCAAAACCCAAGGTGTGAGCCCTGTGC210.29711375212224106No Hit
CCACGTCCCACGAATCCCAATAATGACAGCTTCAGACTTTGTTTTTTTAA200.2829654782116582No Hit
GATCTCTTCCTTGCAGGGAACAGCTTTGGGGCTGGGGGAACTAGAACCCA200.2829654782116582No Hit
GCTGATCAAGATGACACTGAGTAACCCTTCAGAACTAGATGAACTTATGA200.2829654782116582No Hit
CCTTACGGCCAAGTTTCTTCTAGTATGATGGAAAGTTTCTTTTTTCTTTG200.2829654782116582No Hit
CATTTGTAGGTAGTGAGACAAAATTGATGATCCATTAAGTAAACAATAAA200.2829654782116582No Hit
GATTTAAACTGGTGTCTTAAATAGGTTTGATCACATTCAAGGATTATTAT200.2829654782116582No Hit
AGTGTCCTGCCTCTTTGCTAGCAAATGCTTTTAGACACTGTGGCAGTGAG190.2688172043010753No Hit
GGCCAGAATCATGCAGATCCACTCCCGAAAGATGAATGTCAGTCCTGACG180.2546689303904924No Hit
GCTGGTGGGCCCAAGCCTCCAAGTGGGAAGAAAGACTGGGATGATGACCA180.2546689303904924No Hit
GTTCCCATGAACTATATGACTGGCCACACAGGATCTTTTGTATTTAAGGA180.2546689303904924No Hit
CTACTCCCAGACTCCGTACGCCTTCACGTCCTCCTCCATGCTGAGGCGCA180.2546689303904924No Hit
GGGGAGACCTTAAGAATTTTGACACAGTATGTCAAAAGTAATGTCAGCAA180.2546689303904924No Hit
TCTGTAAACTGTGCTAGTGCTGACGATGTTCTGTACAACTTAACTCACTG170.24052065647990947No Hit
GGCTCCCAAACGTACCCAGGCCCCTACAAAGGCTTCAGAGTAGATATCTC170.24052065647990947No Hit
GAATTAACTGTTGAATATGTGGTTTTTTCCTGTAACCAAATTGCTGTAAC170.24052065647990947No Hit
AGACCACCCAGGCCAACATGGTGAAACCCTGTCTCTCTACTAAAAAAAAA170.24052065647990947No Hit
CTTTCCCTACACGACGCGGGCAAGCTTTTGAAATTTGATTTTCCCCTCCC160.22637238256932654No Hit
CTTCTTTAGGCAATGATTAAGTTAGGCAATTTCCAGTATGTTAAGTAACA160.22637238256932654No Hit
GTTGAGGGACAGGATTAGGCAGGGTCTGTCCTGTGGCCACCTGGAAAGTC160.22637238256932654No Hit
GTCTTCACTGCCTCCTTTTCCCTGTCATGCTCATCAGCTTATGGCTTCTG160.22637238256932654No Hit
GTATCTCATAGAAACAAAGACTAACAATTTGTAAACAAAACAATCTGTAA160.22637238256932654No Hit
TTGCAGCAGGAAACTAAATATATTTTGCCAACTTTGGAAAAAGAATTATT160.22637238256932654No Hit
CATTTGGATTTTTTTAAAACAAGTAAATTTCTTATTGATGGCAACTAAAT160.22637238256932654No Hit
CCTGATGCCAGATTTTTTTCATAAATATTCTGCCTACTGTAAATATGGGT150.21222410865874364No Hit
GCCCGCCTCAGGAGGTGGCCGACTGCTGCTAGCGGCCTGCCGCCACGTCA150.21222410865874364No Hit
CTTTTGGAGTCAGAGCGAGAAGCCCGGAGGTTGCGCTGAGCTTGGCTGCT150.21222410865874364No Hit
CTTAACAGTGCAATATTAGAAACCCACAATCTGCCAAAGCAACCTCCACT150.21222410865874364No Hit
TGAGGGGACAGTGCAGCTACAGGATATGCCATCATGGCAGAAAGTTCTAT150.21222410865874364No Hit
GGAAAACAAGAAAGGCATTATAGTGAAATCGAGAACAAGGGAGAGGTTCA140.19807583474816073No Hit
GGATCTAGAAGAATATGTCAAACTATAAACTGCTTGTGATTGTGAATGAC140.19807583474816073No Hit
AGATTGGGTACTTAGTTGCCTGTTACTGAGTTAGCTCTACTCTTTTGGAC140.19807583474816073No Hit
GAAATATAAAGCCCTGCAACCTGCAAAAAAAAAAAAAAAAAAAAAAAAAA140.19807583474816073No Hit
GTGCCCACTACCAGCACCTTGATTCTTCCTCCAGAGTACAGTTCTTGGGG140.19807583474816073No Hit
GGCGCTCGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGACAGGAGAATTG140.19807583474816073No Hit
GGAGCTGAGTAACACCATCGATCATGATGTAGAGTGTGGTTATGAACTTT140.19807583474816073No Hit
GAGGTGTACCGGCATTTCGGCAAGGCCCCAGGAACCCCGCACAGCCACAC140.19807583474816073No Hit
GCATGTGGAGTGGAGGAGATGCTGCCCCTGTGGTTGGAACATCCTGGGGT140.19807583474816073No Hit
AGTTTGGGTGGGGGGTGATCACCAGGGGACCTAGGGAGATCCCCTTCCCA140.19807583474816073No Hit
CCTCTGAGGGCAGGGCACAGACAAACAAAAAGACAGCAGTAACCTCTGCA140.19807583474816073No Hit
GGAGAAAGGGAGCATTCCTGAGGTTCTATACTCAGCAGTAAAAGTAGAGG140.19807583474816073No Hit
CTCTCTAGTATACTGTAATTCTCTCAATTCTGACTAATTCTGATTATTCC140.19807583474816073No Hit
GCACAGACTCCTTATGGCAATAAGACACCAAATTATAAGCAGCACCTCAG140.19807583474816073No Hit
GACCTCAGGTGATCCACCCACCTCAACCTCCCAAAGTGCTGCAATTACAG140.19807583474816073No Hit
GTATTTAAATTGACCAACCTAATGTTACAACTACTTTGAGGTGGCCAAAT140.19807583474816073No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.19807583474816073No Hit
CCCTCTCTGTTGGCCTCAGAGTCACTCCTGCCCCCTCTCCCTGACTTGGT140.19807583474816073No Hit
CTGCCTCCTCTCCAGTGGTCTCCCAGGTGCCAGACCCAAAAGCTTTTCCT130.18392756083757783No Hit
GCTTTTCCTCTCTGCACCTGCGCTCTGGTGACTTGGGGTGGACGCCTCTG130.18392756083757783No Hit
GCTACATTTGAAGCCTGTACAAAAGCTTATCCCTGTAACACATGTGCCAT130.18392756083757783No Hit
GTTGACGTGTATTATAAAAGAACTACTCTACATGCTTCACACCCTAGAGA130.18392756083757783No Hit
ATTTAAAGGATAATAAGGTCCTGTCTTCACTAAGCCATTGACATGAATGT130.18392756083757783No Hit
CATACAAACAATGCAACCAACTATCCAAGTGTTATACCAACTAAAACCCC130.18392756083757783No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCA130.18392756083757783No Hit
GACTGCAGCCCTCTCAGACTTGAGCGTTAATTGGCTTATTTATTTATGGC130.18392756083757783No Hit
CTGCTGGAGATGGTGGAGTCCGTCATGCCGGTGGTGGTGTGCACATTGAG130.18392756083757783No Hit
CGCCTTTTAGAAGGAGAAACTTAAGTGTGGAATGCATTATATGGGCAAAG130.18392756083757783No Hit
GGCATTACTCTGCACTATAGCCATTTGCCCCAACTTAAGTTTAGAAATTA130.18392756083757783No Hit
TGCCCCAGCCAGTCCCCACAGCATAACAGGGTCTCCTTGGCAGCTGTATT130.18392756083757783No Hit
GATTTAGAGACTGGCAAGATTACTGATTTCATCAAGTTCGACACTGGTAA120.1697792869269949No Hit
GGTTGCAGTGAGCCGAGATCGTGCCACTGCACTCCAGCCTGGGCGACAGA120.1697792869269949No Hit
ACTTGGGAGGCTGAGGCAGGAGAATGGCATGAACCCAGGAGGTGGAGCTT120.1697792869269949No Hit
GACCTCACCTGGAAACTCACCTTCAGCGCCTCAGCCCTGGACTGTTAGAG120.1697792869269949No Hit
GGAGATTCTGAAGAAGTTCATGGATCAACATCCGGAGATGGATTTTTCCA120.1697792869269949No Hit
GGTGCCAAGTCTAGTGGTGGCAGGGAAGACTTGGAGTCATCTGGACTGCA120.1697792869269949No Hit
GGGCCTTCACAGAATGATGGCACCTTCCTAAACCCTCATGGGTGGTGTCT120.1697792869269949No Hit
GGCGTAGTGGCGGGCACCTATAATCCCAGCTACTCAGGAGGCTGAGGCAG120.1697792869269949No Hit
GGTCCCAGCACCGATGGCATTCCCTTTGCCCTGAGTCTGCAGCGGGTCCC120.1697792869269949No Hit
ATTCTTTGAGACATCAGCTGGGTAAAACATATTTTGTTTCAATATTAAAA120.1697792869269949No Hit
CTTTGAGTCTGTGGTTCACCACGAGCCCATCAGTGTTTCTTTGAGGTGAT110.155631013016412No Hit
GTTATACTGCTCATGACTGTATTTAAAAAGGAAGAATGATTGAAAACTAT110.155631013016412No Hit
GATTTGTTCTTGCAGATGTTCTTCTATCAAATACCTTATTTTACCTTACA110.155631013016412No Hit
GGCCTTTCGGGAGGGAGAGAATACCCAGGTTGCAGAGCCTGAGGCCTGTG110.155631013016412No Hit
CTATGGTTTGTGGTACAACTTCATTCTATAGAATATTAAGTGGAAGTGGG110.155631013016412No Hit
GCAGGGAATGGAACTTTAATGGAAGAACCACTTTTATCTATTCTTTTTAT110.155631013016412No Hit
GTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCTTCGAAGCGAAA110.155631013016412No Hit
GATTCAATGTAAAGCAAACTTTGAAAATAAGCTAATAGTCCTTAAAGAAA110.155631013016412No Hit
CAGTTTTGGCTTCTTAAACTTCATATTTGGGTAGGTTAAGCTGCCATACG110.155631013016412No Hit
CCTGGGACTTGGCAGTCATGCCGGGTGATGGTGTTCCTGCGGAGACCCTC110.155631013016412No Hit
GGGTCTTTGCAGATTCTAAAGTTATACATGAATACATCAAAGTGGACAAA110.155631013016412No Hit
CCCAAGGGAGCAGCAGTGGAGAGCATCCCAGTGTTTGGGGCACTGTGTTC110.155631013016412No Hit
CTGCCAAGAAGGAGGTCAGCAAGGCCCCTGAAGACAAGAAGAAGAGACTG100.1414827391058291No Hit
ATACAGGATTTAAACCTTTCTAAATAGATCCTAAAACTGTCTCTCACATT100.1414827391058291No Hit
TCCCAGCTACTCGGGAGGCTGAGGCAGGAGGATGGCGTGAACCCAGGAGG100.1414827391058291No Hit
CAATAAAACATTAATGCTAACACTTTTTAAAAAAAAAAAAAAAAAAAAAA100.1414827391058291No Hit
AGTGTAAACTGAATCCAATTCCTGGCCCCCAGAGCAAATAAGCTACTGTC100.1414827391058291No Hit
GTTTAAAACCCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.1414827391058291No Hit
GAGTCGACCCTGCACCTGGTCCTGCGCCTGAGGGGTGGCTGTTAATTCTT100.1414827391058291No Hit
CTACTGCACTCCAGCCTGGATGACAGACCGAGACTCCATCTCAAAAAACA100.1414827391058291No Hit
TTCTCCTCTCTGTTCAGGTAAATGTCACACTGTGCCCAGAATGGATGACC100.1414827391058291No Hit
GTTCTATCAATTGATTTCAAACCTTCAGAAATAGAAGTTGGAGTAGTGAC100.1414827391058291No Hit
GATTAGTCAATCGATTAAAGTTTGATAATTAAAAAAAAAAAAAAAAAAAA100.1414827391058291No Hit
GGATTAGTTCTTTTCAGCATGTTCCTTCTGTATGATACCCAGAAAGTAAT100.1414827391058291No Hit
CACCGTGAGGCCAAGATCCCCTCAGAAGGCAGGAGTGCTGCCCTCTCCCA90.1273344651952462No Hit
ATATCCCTCCCGTGTGTGGTTGGAAAACTTTTGTTTTTTGGGGTTTTTTT90.1273344651952462No Hit
CTTTCCCTGCTGTTACCTAATTACAAATTGGATGGAACTGTGTTTTTTTC90.1273344651952462No Hit
GCATTTTATGAGTGCGTTGGATAAGGCCCTTACGTCAGTTGTAAATTACA90.1273344651952462No Hit
ACTAAGAGCCTTCTGAGCCCAGCGACTTCTGAAGGGCCCCTTGCAAAGTA90.1273344651952462No Hit
GTTTCTGTATGTTAATGACTTGTTGGGTAGCTATTAAGGTACTAGAGTTG90.1273344651952462No Hit
GGATTAACTTCTGCCAGCTCAGACCATTTCCTAATCAGTTGAAAGGGAAA90.1273344651952462No Hit
CTTTTGTAACAAACTCCTACCAACACTGACCAATAAAAAAAAATGTGGGT90.1273344651952462No Hit
CCTGGAGATCGGCCTGCTGCTCCGCAACTTCGACCGCTACGGCGTGGAGT90.1273344651952462No Hit
CCAACTGGAGCTCTCCTTTGCTTGTCCTCTTTGCCTTCGGTAATATGTAT90.1273344651952462No Hit
GAATAATGGTAGCCTAAACTTAATATTTTTATAAAGTATTGTAATATTGT90.1273344651952462No Hit
CACTCTGAATAAAGGACAGTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAA90.1273344651952462No Hit
GGACAGATCACTTGAGGTCAGGAGTTCGAGACCAGCCCAGCCAACATGGC80.11318619128466327No Hit
ATCCATACCTGTGCATGAGCTGTATTCTTCACAGCAACAGAGCTCAGTTA80.11318619128466327No Hit
GGTGTTGACTTGAAAAATAAAGTGTTGATTGGCTAGAAAAAAAAAAAAAA80.11318619128466327No Hit
ACTGCTACATGTAGTGAGATTATTTTTCAAGCTGAATTGAAACCACACAT80.11318619128466327No Hit
GGGTTTCACCATGTTGGCCAGGCTGGGCTCGAACTCCTGGACCTCAAGTG80.11318619128466327No Hit
GTTCTAATAATGTCCTTAAATTTTATTTCCAGCTCCTGTTCCTTGGAAAA80.11318619128466327No Hit
GCCTTTTGCTGGTGTTTCTGGAATTTGCTTTCCCTCACCTCTCACTTCCT80.11318619128466327No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA80.11318619128466327No Hit
GCTATGAAGTAATAGAGTTTGCTTAATCATTTGTAATTCAAACATGCTAT80.11318619128466327No Hit
GGTTCAGGCTTCACCCACTGGAAAGAACCACCATCTGCTCTAACCATGTA80.11318619128466327No Hit
ATTGTACTCCAGCCTGGGTAACAAGAGCGAAACTCCATCTCAAAAAAAAA80.11318619128466327No Hit
GGCTCTAAGTAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.11318619128466327No Hit
CAAACCATTTTATTTTTTGGGCCATTACCCCATACCCCTTATTGCTGCCA80.11318619128466327No Hit
GAATGCAGGAGAAGGGTGGAGAGGAGGCAGGGGCCGAGGGGGTGCTTGGT80.11318619128466327No Hit
GCTGTAAACTGTAAAAAGTTTATGGAGACTTAAAGTCTTGATGTTGTGAA80.11318619128466327No Hit
GACATGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.11318619128466327No Hit
GAACAAACCATGCTGGGTTAATAAATTGCCTCATTCGTAAAAAAAAAAAA80.11318619128466327No Hit
ATAAACTGCCCTGCTGAATTTGAATATACTACGGAAAAGACCTGTTCTAT80.11318619128466327No Hit
CCTCCTCACAGTTGCCATGTAGACCCCTTGAAGAGGGGAGGGGCCTAGGG80.11318619128466327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)