FastQCFastQC Report
Wed 8 May 2024
EGAF00002877262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114700
Total Bases7.5 Mbp
Sequences flagged as poor quality0
Sequence length66
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42403.696599825632084No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6890.6006974716652136No Hit
GCTCAAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG2040.17785527462946818Illumina Single End PCR Primer 1 (100% over 41bp)
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGC2010.17523975588491716No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)