FastQCFastQC Report
Tue 7 May 2024
EGAF00002877319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23729
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5392.271482152640229No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2260.9524210881200219No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC510.21492688271735008No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG410.17278435669434025No Hit
ACCATGGTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTC350.14749884108053438No Hit
GATTATATGTCATACCTCCATTGGGGAATAAGCATAACCCTGAGATTCTT340.1432845884782334No Hit
ATTGTATACCGCAGATTCAGGCATGGATTCCGTGAAGGAACAACACCTAA330.1390703358759324No Hit
GTCTTGGGGAGTCGGATGATGCAGCTCGTGAGCAGTGGCGTAGAAAATGC320.13485608327363144No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA310.13064183067133045No Hit
ATTATTGGGTCATCAGAATTTGAAACAAAAAATCAAGCATGTTGTGAAGT310.13064183067133045No Hit
AAACCAAGCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAG310.13064183067133045No Hit
GTGTATATTTCCAATGAAATTTTTTTGCAAGCACACTTGTAACCATATGT300.12642757806902946No Hit
TGTTAAGATAGTTAAGCGTGCATAAGTTAACTTCCAATTTACATACTCTG300.12642757806902946No Hit
TTATTTTAGGAATGTATTCATTGTGGTACTTTTAGAAATGATTATTTGTG300.12642757806902946No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAA300.12642757806902946No Hit
TGATGAAACAGTGCCTCTCTGATCACTTTTAAAAGTATATTTTAGACCTC290.12221332546672847No Hit
GTTTTGTGCTGGGCATGGCCAGCCTTTCCCAACCCAGGCTCTGGGGAACT290.12221332546672847No Hit
CTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTAC290.12221332546672847No Hit
ATACTACAGAGTGTGCACACTGGCTATCATTGATCCAGGTGACTCTGACA290.12221332546672847No Hit
TCTACAGACAGTTCAAGGAAATTGTTGCAGTTATTGAAACGGTGTGAAGA280.1179990728644275No Hit
GAAAATAATGGAAAGGATTACAAAGTTAAGAGAAGGCTTAAAAAAGGAAT280.1179990728644275No Hit
GGCTACCCTGCCAAGCGCAAGAGAAAGTATAACTGGAGTGCCAAGGCTAA280.1179990728644275No Hit
ATCCCAGGGAGTGCAAGCTTAGAGCGTCTGGCCAAGAATCAGAGGAAGTG280.1179990728644275No Hit
ATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTATGTACTTAGCTGTA270.11378482026212651No Hit
CACTAAGACGACAGATAATCAAACTAAATAGACGTCTACAACTTCTGGAA270.11378482026212651No Hit
CCATGTATTTGGAGTCTGAGACAGTAATATGGATGTTTCATGAATAGATT260.10957056765982552No Hit
ATAGTGGACAGTCCAAGAGTGATAAGGCCGCAGCCTTGGGGACAAAAATT260.10957056765982552No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA260.10957056765982552No Hit
GTATTGGCATGCTTGCCAGTGTACCTGCTGGTGGGGCTGTAGCCGTCTCT260.10957056765982552No Hit
GTACGTAGGTACAGTTGGAGCACTATATGTACTCTCTGGACTACTTTGGA260.10957056765982552No Hit
GTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCC260.10957056765982552No Hit
ATATATGATTTTCTTTAAGGATGGTTTAGAAATGGAAAAATGTTAACAAA260.10957056765982552No Hit
AAATTGTACAATTTAGTAGAAATGGACCAATTCCCTGAAAATCACGAACT250.10535631505752455No Hit
CTCACCGGCCGCACGCCATGAGGGCCCTGTGGGTGCTGGGCCTCTGCTGC250.10535631505752455No Hit
TAGAAAAGGCTTGCCAATCTATTTATCCTCTCCATGATGTCTTCGTTAGA250.10535631505752455No Hit
GCTGGGGAGTCCCTGCCACACTCAGTCCCCCACCACACTGAATCTCCCCT250.10535631505752455No Hit
GATGAAACATTAAATTGTCTTCCTCGATTCTCCATCGGGTGTAGAGTTTT250.10535631505752455No Hit
TCTATATACTCCGTCCAATATAGATAATGTTTTAATAACAACTGTGGGAT250.10535631505752455No Hit
ATATAACATATCTTGAGCATTCTTGACAGACTCCTGGCTGTCAGTTTCTC250.10535631505752455No Hit
CCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG250.10535631505752455No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC250.10535631505752455No Hit
GGTCCAGCAGAAAAGAGGCTGAATCTTCTCCATTTGTTGAGCGACTTCTG250.10535631505752455No Hit
GATTTAAGGAGAGGGTGTTTCCTCTCATCCATTCTCATTAGCAAGAGAGG250.10535631505752455No Hit
GTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGTAAACAAACAAGT250.10535631505752455No Hit
GACTACCTAAATGCAGAATCACGAGTGAACAGACGTCAGATGCCTGATAA250.10535631505752455No Hit
TGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGA250.10535631505752455No Hit
ACTGACAACGAAGGCCGCGCCTGCCTTTCCCATCTGTCTATCTATCTGGC240.10114206245522357No Hit
TTGCCCGGCTGTGATTGCTGCCAGGCACTGTTCATCTCAGCTTTTCTGTC240.10114206245522357No Hit
CCTCAGTGCTGTGAGCACTGTTGCTCAGGAGTGACAAGTTGCTAATATGC240.10114206245522357No Hit
TGGTAGAATTTCACAGCAATAAAAAAAGAAAATTGACGCCAGATCATAAC240.10114206245522357No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT240.10114206245522357No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG240.10114206245522357No Hit
CCACCTGACCAACCTCCACAGGCTGGGTGGCCCGGAGGCTGGGCTGGGCG240.10114206245522357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)