FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002877319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23729
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4741.9975557334906655No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2220.935564077710818No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT500.2107126301150491No Hit
ACCATGGTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGTCCTCTGCCAGCCTAC340.1432845884782334No Hit
GATTATATGTCATACCTCCATTGGGGAATAAGCATAACCCTGAGATTCTTACTACTGATGAGAACATTATC320.13485608327363144No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC320.13485608327363144No Hit
ATTATTGGGTCATCAGAATTTGAAACAAAAAATCAAGCATGTTGTGAAGTTGAAAGATGAAAATAGCCAAC310.13064183067133045No Hit
ATTGTATACCGCAGATTCAGGCATGGATTCCGTGAAGGAACAACACCTAAACCCAAGAGGGCAGCTGTTGC310.13064183067133045No Hit
GTGTATATTTCCAATGAAATTTTTTTGCAAGCACACTTGTAACCATATGTGTATAATTAACAAACCTGTGT300.12642757806902946No Hit
GTCTTGGGGAGTCGGATGATGCAGCTCGTGAGCAGTGGCGTAGAAAATGCACTCACCAAATCAGAGCTGTT300.12642757806902946No Hit
ATACTACAGAGTGTGCACACTGGCTATCATTGATCCAGGTGACTCTGACATCATTAGAAGCATGCCAGAAC290.12221332546672847No Hit
TGTTAAGATAGTTAAGCGTGCATAAGTTAACTTCCAATTTACATACTCTGCTTAGAATTTGGGGGAAAATT290.12221332546672847No Hit
TTATTTTAGGAATGTATTCATTGTGGTACTTTTAGAAATGATTATTTGTGAAGTTAATACTCAGATTTTTT290.12221332546672847No Hit
GGGAAGGAAAGAACTTGCATGTTGGTGAAGGAAGAAGTGGGGTGGAAGAAGTGGGGTGGGACGACAGTGAA280.1179990728644275No Hit
ATCCCAGGGAGTGCAAGCTTAGAGCGTCTGGCCAAGAATCAGAGGAAGTGGCTGATGACTATCAGCCAGTT280.1179990728644275No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGC270.11378482026212651No Hit
TCTACAGACAGTTCAAGGAAATTGTTGCAGTTATTGAAACGGTGTGAAGACGGATTCTTTGGTTGATAAAT270.11378482026212651No Hit
GGCTACCCTGCCAAGCGCAAGAGAAAGTATAACTGGAGTGCCAAGGCTAAAAGACGAAATACCACCGGAAC270.11378482026212651No Hit
TGATGAAACAGTGCCTCTCTGATCACTTTTAAAAGTATATTTTAGACCTCTCTACCCTAGTTAACATTCCA270.11378482026212651No Hit
ATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTATGTACTTAGCTGTACTATAAGTAGTTGGTTTGTAT260.10957056765982552No Hit
GTACGTAGGTACAGTTGGAGCACTATATGTACTCTCTGGACTACTTTGGACAGAAGTAGGTTTTTGAATGT260.10957056765982552No Hit
GTTTTGTGCTGGGCATGGCCAGCCTTTCCCAACCCAGGCTCTGGGGAACTTGTAATGGGGAAGGCCTCAGG260.10957056765982552No Hit
CTACAATCTTCCTAGGAACAACATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAG260.10957056765982552No Hit
ATAGTGGACAGTCCAAGAGTGATAAGGCCGCAGCCTTGGGGACAAAAATTATTGATGAAGATGGCCTGTTG250.10535631505752455No Hit
GATGAAACATTAAATTGTCTTCCTCGATTCTCCATCGGGTGTAGAGTTTTTAAACTATCAATGGCATTTCA250.10535631505752455No Hit
AAATTGTACAATTTAGTAGAAATGGACCAATTCCCTGAAAATCACGAACTTCTAAAACTAACCAAGATGAA250.10535631505752455No Hit
CCATGTATTTGGAGTCTGAGACAGTAATATGGATGTTTCATGAATAGATTTGAGGAAAAAAAGAATACACA250.10535631505752455No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG250.10535631505752455No Hit
CCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC250.10535631505752455No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTA250.10535631505752455No Hit
CACTAAGACGACAGATAATCAAACTAAATAGACGTCTACAACTTCTGGAAGAGGAGAACAAAGAACGTGCT250.10535631505752455No Hit
ATATAACATATCTTGAGCATTCTTGACAGACTCCTGGCTGTCAGTTTCTCAGTGGCAACTATTTACTGGTT240.10114206245522357No Hit
GAAAATAATGGAAAGGATTACAAAGTTAAGAGAAGGCTTAAAAAAGGAATGAAATATAGGGTTGGATAAAA240.10114206245522357No Hit
GTCTACAATGTTACCCAGCATGCTGTTGGCATTGTTGTAAACAAACAAGTTAAGGGCAAGATTCTTGCCAA240.10114206245522357No Hit
TCTATATACTCCGTCCAATATAGATAATGTTTTAATAACAACTGTGGGATAAAAGTTATCTTCCCCTTGGA240.10114206245522357No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGA240.10114206245522357No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAA240.10114206245522357No Hit
GGTCCAGCAGAAAAGAGGCTGAATCTTCTCCATTTGTTGAGCGACTTCTGAAAAAGGGCTATGAAGTTATT240.10114206245522357No Hit
GATTTAAGGAGAGGGTGTTTCCTCTCATCCATTCTCATTAGCAAGAGAGGCTTTCTGTGAGCTTCTCACAC240.10114206245522357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)