FastQCFastQC Report
Tue 7 May 2024
EGAF00002877326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29610
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8682.931442080378251No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1970.6653157716987503No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC970.32759202971968926No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG670.22627490712597095No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA660.22289766970618033No Hit
GAGGTGAACTTCCAGGAGTATGTCACCTTCCTGGGGGCCTTGGCTTTGAT500.16886187098953057No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC370.12495778453225262No Hit
GATGAAACAGGAACTGGAAGCTGAATATTTGGCAATATTCAAGAAGACAG360.12158054711246201No Hit
CTGTTGGAAAACGCTTCAAAGAGGCAAATAACTTCCTGTGGCCCTTCAAA350.1182033096926714No Hit
GGTTAAGCACGTTTTGGGGATAATTTTCTCTTCTTTAGGCAATGATTAAG340.11482607227288079No Hit
GTATAGGATGGTGGATACTTACTATTAAGTTACTGCACAATTCCTAGAGG330.11144883485309016No Hit
TCCAAGATGTTCCTGCGCGGGGACTCAGTCATCGTGGTCCTGCGGAACCC320.10807159743329957No Hit
GGGCTGCACTGCCACCCTGGGCAACTTCGCCAAGGCCACCTTTGATGCCA310.10469436001350894No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT300.10131712259371835No Hit
CTCTAAACTTATGTGAACAGTTTTGAGTTTATGAATTCTAGAAACTAAAA300.10131712259371835No Hit
ATATTAATGAATATTATTATATTCATAGCACTTCATCATTACATAACAGA300.10131712259371835No Hit
TAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGAT300.10131712259371835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)