FastQCFastQC Report
Wed 8 May 2024
EGAF00002877362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326955
Total Bases23.2 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52181.5959382789680538No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA11540.35295377039653775No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8430.25783364683213283No Hit
GTCCAAGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG4970.15200868621064062Illumina Single End PCR Primer 1 (100% over 41bp)
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGC3960.12111758498875992No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG3890.11897661757734246No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA3610.11041274793167255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)