FastQCFastQC Report
Tue 7 May 2024
EGAF00002877383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580
Total Bases112.1 kbp
Sequences flagged as poor quality0
Sequence length71
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35822.658227848101266No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA181.139240506329114No Hit
AGATAAAGTGATTTCAGGAATAGCAAGAGAAAAACTACCAAAGGTAGACA150.949367088607595No Hit
CCAATTTCACCTCTGTCTCCAGTATCACCACAAAATTGTTCTTCCTTGGA140.8860759493670887No Hit
GGCTTCTTCTAGTCGCAGTCCCCAAGTACTTGCTGAATCCTGTATGGACA130.8227848101265823No Hit
ATGTCTATAGTTTTGGCCTTAACTATGCCAATCTAATTATAATTCCCTGT100.6329113924050633No Hit
GACTTTCTAGTGTACAAGACACCATTGTGTCCAACTGTATATAGCTGCCA100.6329113924050633No Hit
CCTCGGCATGTACTGTAGTCACTCAGTATTTGTATATGTTGCTAGAATTT100.6329113924050633No Hit
CTTCAGACCAGCATCACAGATTATAACCCTCCGTAAATCATCTGCATCCC90.569620253164557No Hit
GTCATAGGCTTCTTTCAGTCTTTATGTCACCTCAGGAGACTTATTTGAGA80.5063291139240507No Hit
GCAGGGACTCTGTCCCCACACTCAAAAAGACTCAGCTCACTCAATGAGAG70.44303797468354433No Hit
GGCTAGGATCGGGTTCTGGCAGGGAGAACCTGCCCTGCCACTGGCCCCAT70.44303797468354433No Hit
GTTTTGTGGACATATTTTCTCTTAATATTGTCTACATGCTAGCTTTCAAG70.44303797468354433No Hit
GGTGTGATGGCACGTGCCTGTAGTCCCAGCTACTCAGGAGACTGAGGCAG70.44303797468354433No Hit
TATGTATATGTGTATATGTATGTGTATATATGAACATTATATATATGTGT70.44303797468354433No Hit
CTCGAGTGCCCCTGTCCCACCTGGCTCCCCCTGCTGGTGTCTAGTGTTTT70.44303797468354433No Hit
GCTAACTTAGCATTAACCTTTTAAGTTAAAGATTAAGAGAACCAACACCT70.44303797468354433No Hit
GCTTATACGAAATAATTGTTGTTCTGTTAACTGAATACCACTCTGTAATT70.44303797468354433No Hit
CAGTACATCAGAACTCATGCCAACAGTCTTTATGATGGGATTAAGGTGGA60.37974683544303794No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG60.37974683544303794No Hit
ATAGAATAAAGTTAATACCAAACTTGAAAAAAAAAAAAAAAAAAAAAAAA60.37974683544303794No Hit
CCTTCTCCAAGCTCTGAGAGCCCTCGCGTCCCAGGCCCCAGCCAGGGGGC60.37974683544303794No Hit
CCCCTCACCTCCTGCCACCAGGCCTGTGTGGACAATTGGGAATAATTCAG60.37974683544303794No Hit
ACCCCGACATGTCCTAACATTTTTCTCTATCAGTTGGTACCATAAGTTGT60.37974683544303794No Hit
GATTCCCAGCAAACCTGCTCCTCTTGTTCATAGTATTATCATGAAGATTA60.37974683544303794No Hit
CCTCTTTTGGAATATATTAAAAATAGAAAATTAGAAAAGCAGAGAATTCG60.37974683544303794No Hit
GTATAGATGCAAGATATTTATATATATTTTTGGTTGTCAATATTAAATAC60.37974683544303794No Hit
GACAATTATGTTCCTGAGGTCTCAGCCTTGGATCAGGAGATTATTGAAGT50.31645569620253167No Hit
ACACGACGCGGGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGG50.31645569620253167No Hit
GTCCCTCTCCAAGCGCCGCCCCTGGCGTGGGCTCGGTGGAGCAGACCCCG50.31645569620253167No Hit
GTTGCACAGGCTGGTCTCAAACCCCTGAGTTCAAGGAATCCTTCTGTCTC50.31645569620253167No Hit
GGGCGGGCAGATCTCTTGAGGTCAGGAGTTCGAGACTAGCCTGGCCAACA50.31645569620253167No Hit
CAAAGCTGAAGGAGAAGTATGAGAAGGATGTTGCTGACTATAAGTCGAAA50.31645569620253167No Hit
CACCCCTGCATGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.31645569620253167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.31645569620253167No Hit
GACACTATTACCAAAGATTGTTGGTTAGGCCAGATTGACACCTATTTATA50.31645569620253167No Hit
GAAGATTAACCCTGAGAATTCCAAGCTGTCAGACCTGGACAGTGAGACTC50.31645569620253167No Hit
GTAAAAGTTCTAAAATGAAGTCACACAAGACAATTATTCTTATGCCTAAG50.31645569620253167No Hit
GTACTAATCTCCAATTAAAAAATTTTTTAACATGTAAAAAAAAAAAAAAA50.31645569620253167No Hit
GCTCTCTGGGCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.31645569620253167No Hit
GTGTGGGTCAAGATCAGCTCCAGCTGGGTCTGCCTCCTGCTTTACGTCTG50.31645569620253167No Hit
GACATGGGCTTTGGTCTTTTTGACTAAACCTCTTTTATAACATGTTCAAT50.31645569620253167No Hit
GCACTTGGATTATGGATCTGAATAGAGAAATGCTTACAGATAATCATTAG40.25316455696202533No Hit
TCTACAGACAGTCAATGTGGATGAGAACTAATCGCTGATCGTCAGATCAA40.25316455696202533No Hit
ACATACAATAATATTAAACTTATGATGATATATGTACTCACTAAATTACA40.25316455696202533No Hit
ATAGAGATTATTTCTGTAAATGAAATTGGTAGAGAACCATGAAATTACAT40.25316455696202533No Hit
CCAATAAGGAAAAGCTTGAAGCCACCATTAATGAATTAGTCTAATCATGT40.25316455696202533No Hit
ACGCGGGGAAGAAGAGGCTGGGTGTGGTGGCTCACGCCTGTAGTCCCAGC40.25316455696202533No Hit
TGGCCTGGGTAGGACATTTCCCAGCAAGGGCTGGAGGAGTTGCCGTGCCT40.25316455696202533No Hit
AAACCAAATATGTTAAAGTTTAAGTGTGCAGCCATAGTTTGGGTATTTTT40.25316455696202533No Hit
GAAAGAGATTGTAACAAATTTCTTGGCTGGATTTGAAGCTTAAACTCCTG40.25316455696202533No Hit
ATGTGGCCATTAGCCTTCGGTTCTGGCATGGGATTAGGAATGGCTTATTC40.25316455696202533No Hit
GGATTAGGCACGCGCCTGTAATCCTCAGCTCCTTGGGAGACTGAGGCAGG40.25316455696202533No Hit
CTCTTGTCCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.25316455696202533No Hit
ATCCTGTAGTGTTCCTGGAGAAGCTAGAGCCTGATTGTAGGCTACTACTC40.25316455696202533No Hit
GTGTTGCCAATGTGACAGCATCAAGAGGTGGGGTCTTTAAGAGATCACTA40.25316455696202533No Hit
TAAATATACTAAAGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.25316455696202533No Hit
CTACTAAACATACAAAGAATTAGCCGGGCGTGGTGGCGGGCGCCTGTGGT40.25316455696202533No Hit
GCAACTCCATGCAAACCATCCAAAAGACCACCACCCGCCGGATAGTGGAT40.25316455696202533No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC40.25316455696202533No Hit
AGAACCTGTATTTTGAAAAACACATTCATGTATATTTATTCCTGGAATTA40.25316455696202533No Hit
ATACAGTGCTTAGGATGTCTTTCACAGAGCTTATTAAAAAGATGAAACCT40.25316455696202533No Hit
CTTGCAAAATGTTTGGATGTAATGTTAAAGCGCAATGTGCAAAATTTAAA40.25316455696202533No Hit
GCATTGAGAATATAATATGAAATTATGAAAAAAAAAAAAAAAAAAAAAAA30.18987341772151897No Hit
AGATTGTTTTATTTGAATGGGGAAAATACCCTTTAAATTATGACGGACAT30.18987341772151897No Hit
GGTCTAATGTATCCACAGGCTGTTGTCTTATTAGTAAATGCAAAGTAATG30.18987341772151897No Hit
GTTATACAAACCATATACATCTGGTTACCAAACATAAATGCTGAACATTC30.18987341772151897No Hit
CTGTGCTATAATGTACTGAAACCACCATATTACAGAAATATTTACTACAT30.18987341772151897No Hit
TCAGTATTGAGCCAGGAGTTGAGGTGGAAGTCACCATTGCAGATGCTTAA30.18987341772151897No Hit
CAATAAATAAAGTTCTTTTTTAGTTCCAAAAAAAAAAAAAAAAAAAAAAA30.18987341772151897No Hit
GGCCTTCCGGGCAGATGACACCTTTGAGGCCCTGTGCATCGAGCCGTTTT30.18987341772151897No Hit
GTCCTGGTAGACATCAGTTGTTTAAGAAGCCTCAATCCCAGTTAATTGGT30.18987341772151897No Hit
ATAAAAATGAAAAGACGCTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18987341772151897No Hit
GTTGAAACCGTGTACTTACCAATAAACTATTTGCTATTCAAAAAAAAAAA30.18987341772151897No Hit
ATATACACACCACACACACACACCACATACACCACACACACACGTTCCCA30.18987341772151897No Hit
GGTTGGAGCTGCTGAGACGACGCTCACAGAGCTGAGACGTACAGTCCAGT30.18987341772151897No Hit
AGCCTGGGGGACAGAGTGAGACTTCATCTCGAAAAAAAAAAAAAAAAAAA30.18987341772151897No Hit
CGCGCGCACGCAGGGGTCTTTTAAAACCCGGCCGGAATACAAAGGCCGGG30.18987341772151897No Hit
GGGTAGAGATAGTGATTAAATGTCGACTTTATGCTGTAATAAATTGTCAA30.18987341772151897No Hit
GCACTTGCCCATTCCTTACACCCCTTCCCCATCCTGCTCCGCTTCATGTC30.18987341772151897No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA30.18987341772151897No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATA30.18987341772151897No Hit
CAGCATTGCTGACACATGAGACTAACACGTGCAATTATTTAAAAAGATTT30.18987341772151897No Hit
GTACCCAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18987341772151897No Hit
GTACTTAGCTGTACTATAAGTAGTTGGTTTGTATGAGATGGTTAAAAAGG30.18987341772151897No Hit
TGCCCAAGCTGCTGACGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18987341772151897No Hit
GATGAGGAGGAACCACCTGATCTCCCCTTGGACTGATACCCTTTTCCCAT30.18987341772151897No Hit
ATTTATGAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18987341772151897No Hit
CCTGGGTGACAGAGTGAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAAA30.18987341772151897No Hit
GAGTTCGAGACCAGCCTGGCCAATATGGTGAAACTCAATCTCTACTAAAA30.18987341772151897No Hit
CTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAAT30.18987341772151897No Hit
GCTGAAACAGCTGGACGACCTGAAGGTGGAGCTGTCCCAGCTGCGCGTCG30.18987341772151897No Hit
AAATAAACCATGTTTGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.18987341772151897No Hit
GCAATCTCTGCTTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCAGA20.12658227848101267No Hit
CTGCTGTGCGTGCTGGGCCTGCTACTCTGCGGGGCGGCGAGCCTCGAGCT20.12658227848101267No Hit
GTGCATTTATTGTCTTTCCACATATTGGAGGAATGTCATCTTCCTAAATG20.12658227848101267No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT20.12658227848101267No Hit
CTTTCTTCCTTTGGTAGGAATGGCCTGAGTTGGCGTTGTGGGCAGGCTAC20.12658227848101267No Hit
ATGTGGAACACAAGGTTGAAACTTTTTCTGGTGTCTATAAGAAGCTCACG20.12658227848101267No Hit
TTCCCTACACGACGCTCTTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12658227848101267Illumina Single End PCR Primer 1 (95% over 23bp)
TCTCGAACTCCTGAACTCAAGCCATCCTCCACCTCAGCGTCCCAAAGTGC20.12658227848101267No Hit
ATATCAAAGCAAACACCAGAAAGTTATATCTTTGATGCATTTTTTCAAAA20.12658227848101267No Hit
GCAATCTCTGCTTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGA20.12658227848101267No Hit
CCTTTACCTTTTCTATAAGTTGTACCAAAACATCCACTTAAGTTCTTTGA20.12658227848101267No Hit
GGTGATGACCTGCTTCCTGCTGGCACTGAGGATTATATCCATATAAGAAT20.12658227848101267No Hit
GACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCAC20.12658227848101267No Hit
TGGCATCATTGCACTCCAGCCTGGACAAGAGTGATACTCTGTCTCAAAAA20.12658227848101267No Hit
GCATTAATTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
ATTCCACGATGCCAAATTCATGGCAGACATTGATCTGGATCCAGGCTGTA20.12658227848101267No Hit
ATTCGGTGTTGGCAAGAAACGGCTTTACTTACAAAACAGACTCTTTACCT20.12658227848101267No Hit
AGGTTAGGGTACCCAGTGTCTTCCAAACTAAAGTATTGTAATATTACCCT20.12658227848101267No Hit
ATATTCCTTTATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
CTCTAGAGCCAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
GCACTTGCCCATTCCTTACACCCCTTCCCCATCCTGCTCCGCTTCATATC20.12658227848101267No Hit
GCAATCTCTGCTTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCGGA20.12658227848101267No Hit
CCAACAGTGATGGTCAGCTAGATTTCTCAGAATTTCTTAATCTGATTGGT20.12658227848101267No Hit
GTCAAGTTTGAAGAGAGATACAAGACAGGCAAGAACAAGTGGTTCTTCCA20.12658227848101267No Hit
CTTCTAGTATGATGGAAAGTTTCTTTTTTCTTTGCTCTGAATAAAACTGA20.12658227848101267No Hit
GCCTCGGGAGTGGGAATTGCTGGTACAAAGACCAAAACAACCAAATGCCA20.12658227848101267No Hit
GTGTAACACTGCAAGAAGTCTTGAGAAAAAGATTATTGTAGTGTTCAAAA20.12658227848101267No Hit
GAGGAGAGGCACCCGATATATGTTCTCTAGGCCTTTTAGAAAACATGGAG20.12658227848101267No Hit
GCCAACTTTCCTACAAGCATGACTGCAGACGTGGAAGAGAAAAGGCAGAT20.12658227848101267No Hit
GTGCGTGAGTTCCTGGAGAAGAACTATACTGACGAAGCCATTGAAACAGA20.12658227848101267No Hit
CGTTAAAGCTTTCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTC20.12658227848101267No Hit
GGAATACACGTCTTCGGTTGGTGGCATTCGAACTCAAATCGGTCACATCG20.12658227848101267No Hit
ATATTGACAAGTGTCTTTTTGAAATAAAGAACCAGTCCCTCCAAAAAAAA20.12658227848101267No Hit
ACTTAAAGCATCATAGTCCTTTTATATTTGTCTTTGTATTTTATAAGATA20.12658227848101267No Hit
GTGAGCCAAGATCACGCCACTGCACTCCAGCCTGGGTGACAGACTCTGTC20.12658227848101267No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
GCAACAGATATCTCTTGCAACACAAATGGTTAGAATGATTTTGAAGATTG20.12658227848101267No Hit
GTTTGTGTTTATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
CTTCCGATCTGTTCTTACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
GTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
GTATAACTTTGAATAAATGGTTAATGTTCACTTAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
TTCCTTGTGTGATGCCTCCTTAGGCACACAGGCAATGTCTCAAGTACTTT20.12658227848101267No Hit
GTTTCATGCCGATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA20.12658227848101267No Hit
ACCTTAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCCTG20.12658227848101267No Hit
GAGGATAGATGATCTCTGTGGGTCGGAGCATTGGGTACAAAAAAAAAAAA20.12658227848101267No Hit
GTTCTGCACTTAATAAATGGTTTTGCTACAAAAAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA20.12658227848101267No Hit
TTTCTGTTTTGTAATTTAAGAAGAGCTATTCATCACTGTAATTATTATTA20.12658227848101267No Hit
CTTCTACTCTTTGAGACATCACTGGCCTATAATAAATGGGTTAATTTATG20.12658227848101267No Hit
GTGTTTGTCTGACAAAAAAAAAAAATAAAATAAAATAAACTGTTTTGAAA20.12658227848101267No Hit
GCTACCTTATCAAGGACGGCAAGCTGATCAAGAACAATGCCTCCACTGAC20.12658227848101267No Hit
GGCCTTGGCGGTTCCCCGTTTCCTTACGTAACCTCAGTGTTCACGCTGTT20.12658227848101267No Hit
GTATAAATGTGCCCCAAATATTACATGGCATGTACACATACTAAAAACAC20.12658227848101267No Hit
ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAA20.12658227848101267No Hit
CCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGATCAAATA20.12658227848101267No Hit
CCCTGATGGACCCTCAGTCTTCTCATGAATAAATTCCTTCAACGCCTAAA20.12658227848101267No Hit
CAGTTGGGCTTTGTTCATTCTAGCCACGGGGGTGAAAGGGATCTTTTGCA20.12658227848101267No Hit
CTACATATTTACCACAACACAATGGGGCTCACTCACCCACCACATTAACA20.12658227848101267No Hit
GTAGTTGACTAGAATGCTCTGTATACTTTATATTAAAAAATGCTTTGCAT20.12658227848101267No Hit
GGTGATAGCATTGCTTTCGTGTAAATTATGTAATGCAAAATTTTTTTAAT20.12658227848101267No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
CCTTATGTACCGAGCAAATGCCAGGTCTAGCAAACATAATGCTAGTCCTA20.12658227848101267No Hit
GTTATAAGGTCTATGATGTGTTTACTTTAAAAATTGCTGTTAAAAGCAAC20.12658227848101267No Hit
CCAAAGGAGCCCTTGAGCTAGGGAGGTGGTGCAGTCAGCCTCGCTCTCAA20.12658227848101267No Hit
CTAAGATGTGAGGAAATTGTGATTTGTTCTCTCCACCACAAATGAATTAC20.12658227848101267No Hit
GTGTTTGTCTGACAAAAAAAAAAAATAAAATAAAATAAACTGTTTTAAAA20.12658227848101267No Hit
GTATTGACTCACCCATCAACAACCGCTATGTATTTAAAAAAAAAAAAAAA20.12658227848101267No Hit
GTGTCCCTCTGCCCCCTTCCATCCCCAACCACATTTGACTGTAGCATTGC20.12658227848101267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAA20.12658227848101267No Hit
GTGTAGCAATTACTGTCTTTAAAAATTAAGTGGAAGAAGAATTACTTTAA20.12658227848101267No Hit
GCTAAAGTTCTGAAATATGGAATGTACTGCCTCTAATTTTTCTTTGTCTT20.12658227848101267No Hit
ATTTATTTCTGTGTAAAGTTATTTAAAAACTGTATTATATAAAAGGCAAA20.12658227848101267No Hit
ATGTTGTAGTTACCTACTGAGTAGGCGGCGATTTTTGTATGTTATGAACA20.12658227848101267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)