FastQCFastQC Report
Tue 7 May 2024
EGAF00002877389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30728
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6562.1348607133558968No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA5771.8777662067170007No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA5541.8029159073158032No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT2370.7712835199166884No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC2060.6703983337672481No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG1950.6346003644884145No Hit
TCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGG1940.6313460036448841No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAA1530.49791720906014064No Hit
GGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGT1460.4751366831554283No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACC1350.4393387138765946No Hit
TTCCCTACACGACGCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTG1110.36123405363186667No Hit
GTATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAA1090.35472533194480604No Hit
GTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTAC1070.3482166102577454No Hit
GCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAA1060.34496224941421505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1010.3286904451965634No Hit
CTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.31241864097891175No Hit
CCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTG960.31241864097891175No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT920.29940119760479045No Hit
CTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAA800.2603488674824264No Hit
CCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.2570945066388961No Hit
ACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCC780.2538401457953658No Hit
CCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAA770.2505857849518355No Hit
CTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCC760.24733142410830514No Hit
GAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATA750.2440770632647748No Hit
TCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATT650.21153345482947147No Hit
AATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG630.20502473314241085No Hit
GGCTACACAATTGTTACACTTTATTCAGATTACAATTAATTAGAGTGATT600.19526165061181985No Hit
AACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTT580.18875292892475917No Hit
GAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAAT580.18875292892475917No Hit
TTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGG530.17248112470710752No Hit
ACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.16271804217651653No Hit
ACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATC500.16271804217651653No Hit
AATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTC480.15620932048945588No Hit
GTTATAGTTTACTCAGCTTCACTGTTACATCCTAGATGAGTATTGTATTC440.14319187711533454No Hit
GAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAA380.12366571205415257No Hit
GTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTC370.12041135121062224No Hit
TCCCTACACGACGCGGGAGTATACTACGGTCAATGCTCTGAAATCTGTGG370.12041135121062224No Hit
CCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.1171569903670919No Hit
CCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAGCACCCCCTCT360.1171569903670919No Hit
CCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.1171569903670919No Hit
GATTTACCCTACCCATACATATCCCAAATAGTTTTTGATCAAAAACATGA350.11390262952356157No Hit
TATTTACCCTATAGCACCCCCTCTACCCCCTCTAGAGCAAAAAAAAAAAA350.11390262952356157No Hit
AGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA340.11064826868003123No Hit
CTGGTAAACTGTCCAAAACAAGGTTCCAAATAACACCTCTTACTGATTTA340.11064826868003123No Hit
GTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTA340.11064826868003123No Hit
GGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTC330.10739390783650091No Hit
TTTTCTATTTGTCTATTTTATACAAAAAGGCTACACAATTGTTACACTTT320.1041395469929706No Hit
ACCCTATAGCACCCCCTCTACCCCCTCTAGAGCCAAAAAAAAAAAAAAAA320.1041395469929706No Hit
CCCATACATATCCCAAATAGTTTTTGATCAAAAACATGAAATAGATCCAC320.1041395469929706No Hit
CCATTACTCAATTCTTAAAAATTAGAAATTGCTGTAGCAGTATTCACTAT310.10088518614944025No Hit
ATTAAAAGTGAAACATTTTGGCATGTTTGTTAATTCTAGTTTCATTTAAT310.10088518614944025No Hit
GTTCTACACCATTACTCAATTCTTAAAAATTAGAAATTGCTGTAGCAGTA310.10088518614944025No Hit
GCTTATTTTAAGCATATTAAAAAGGAAACTAATTGGACCATTTTCTATTT310.10088518614944025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)