FastQCFastQC Report
Wed 8 May 2024
EGAF00002877425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567391
Total Bases40.2 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA57191.0079469008144295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40870.7203145626208383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29070.5123451024073346No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT12370.2180154426136474No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA10920.19245987335012363No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC9870.17395411629722712No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG9220.16249817145495787No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG8860.15615334046539336No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT8090.1425824519599359No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT7950.1401150176862164No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC7810.13764758341249686No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAATCA6130.10803837212786244No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC5690.10028357869617248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)