FastQCFastQC Report
Wed 8 May 2024
EGAF00002877435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543893
Total Bases38.6 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60991.1213602675526253No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA29440.5412829361657532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10490.1928688179476478No Hit
ATTCAAGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG10240.18827232562287066Illumina Single End PCR Primer 1 (100% over 41bp)
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC9920.18238881544715596No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT7660.14083652483117085No Hit
ATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGC5790.10645476224183802No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG5600.10296142807500741No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)