FastQCFastQC Report
Tue 7 May 2024
EGAF00002877537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70709
Total Bases5 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7151.011186694763043No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA2220.3139628618704832No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1030.1456674539308999No Hit
GTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAGCTTTC910.12869648842438727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.12586799417330183No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA810.11455401716896012No Hit
GCCTAAGCCTCTCCCTCCAGCCAATAGGCAGCTTTCTTAACTATCCTAAC770.10889702866678924No Hit
TCCCTACACGACTAAGTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG710.10041154591353292Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)