FastQCFastQC Report
Wed 8 May 2024
EGAF00002877570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399029
Total Bases28.3 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54051.3545381413380984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26980.6761413330860664No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA19010.47640647672224323No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC12650.317019564994023No Hit
GTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAGCTTTC5710.14309736886291474No Hit
GCGACTTCTGAAGGGCCCCTTGCAAAGTAATAGGGCTTCTGCCTAAGCCT4360.10926524137343402No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA4280.10726037455924256No Hit
GCCTAAGCCTCTCCCTCCAGCCAATAGGCAGCTTTCTTAACTATCCTAAC4250.10650854950392076No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT4130.1035012492826336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)