FastQCFastQC Report
Tue 7 May 2024
EGAF00002877590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30276
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3491.1527282335843574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.43929184832870916No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA840.27744748315497425No Hit
GTAATAGGGCTTCTGCCTAAGCCTCTCCCTCCAGCCAATAGGCAGCTTTC480.15854141894569956No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC450.14863258026159334No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCA440.14532963403355795No Hit
ACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACCTAAGGGTC350.11560311798123928No Hit
AACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAA340.11230017175320385No Hit
GTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGT340.11230017175320385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)