FastQCFastQC Report
Wed 8 May 2024
EGAF00002877597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693217
Total Bases49.2 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74101.068929354011803No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA39420.5686531057374531No Hit
GCGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG26430.38126589509489817Illumina Single End PCR Primer 1 (100% over 45bp)
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA12490.1801744619650124No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC11910.17180767349906306No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11840.17079788868420712No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT7890.11381717413162112No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA7150.10314230608885817No Hit
TCCCTACACGACGCGGGGGAGCCATTGTGGCTCAGGCCGGTTGCGCCTGC7000.1009784814855954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)