FastQCFastQC Report
Wed 8 May 2024
EGAF00002877602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233353
Total Bases16.5 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30961.3267453171804091No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA19510.8360723881844244No Hit
GGACCAGACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG7650.3278295115126011Illumina Single End PCR Primer 1 (100% over 41bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5950.2549785089542453No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA5910.2532643677175781No Hit
GATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACC5560.23826563189674016No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA3840.16455755872005073No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC3460.1482732169717124No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC3270.1401310460975432No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2970.12727498682253924No Hit
TCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGACCCGTATTTACC2590.1109906450742009No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG2490.1067052919825329No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)