FastQCFastQC Report
Tue 7 May 2024
EGAF00002877626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007
Total Bases71.4 kbp
Sequences flagged as poor quality0
Sequence length71
%GC23

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35635.352532274081426No Hit
CACAAGTACTCCGGCAGGGAGGGTGACAAGCACACCCTGAGCAAGAAGGA242.3833167825223436No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA201.9860973187686197No Hit
AAATAATAATAATGCTATACTTAAATTACTTGTGAAAACAACACATTTTA111.0923535253227408No Hit
GCTCTTCTGAGACTTCCATTCCTTTCTGATTGGCACACGTGCAGCTCATG90.893743793445879No Hit
ACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCAGAGGTT90.893743793445879No Hit
TTGGCAGGCCAAGGAGGGTGGAAGACGAGGTCAAGAGATGAAGACCATCC90.893743793445879No Hit
GGTGTCAAGCAGTTCCACGACTCCAAGATCAAGTTCCCGGTGGGGCAGCG80.7944389275074478No Hit
CTGGATAATCAGTGTATGTTGCACCAGAATCAGCATTTTTTTTTTAACTG80.7944389275074478No Hit
GAATAAAAAACTCTCACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.7944389275074478No Hit
GACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGC70.6951340615690168No Hit
GCTACTCGGGAGGCTGGGGCAAGAGAATCGCTTGAGCCTGGGAAGTAGAG70.6951340615690168No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG70.6951340615690168No Hit
GCTTTAGAAAAGAAGTAAACTGGATACAAAAAGTTCCATTGAGGAACAGT70.6951340615690168No Hit
GAGCAAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.5958291956305859No Hit
CCTGCAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAAAATGGCTTGAACC60.5958291956305859No Hit
TAATAAACAGCCTCCTAGAGGCCACATTCTATTCTTTAAAAAAAAAAAAA60.5958291956305859No Hit
GTCCCAGCCTCGGGAGGGCACCTCCTTTTCTGTGCTTTCTTCCCTGAGGC60.5958291956305859No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATA50.49652432969215493No Hit
ATATATTCCTTAAAATATACATGGGGGCCTGAATGTCAGCCATCTTTATA50.49652432969215493No Hit
CCACCCAGAGAAGCACACTTTGTGAGAACCAATGGGAAGGAGCCTGAGCT50.49652432969215493No Hit
CCCTGACTTTCTGAAGCTAGAAAAATGTTGTGTCTCCCAACCACCTTTCC50.49652432969215493No Hit
ATTATAGCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.49652432969215493No Hit
GTGGAAGGACCAATCACCTCTGATATTCTGTGGAAGGTTTTGGGGTCAAA40.3972194637537239No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA40.3972194637537239No Hit
GCGACTGAAGAAGATTCGGGCCCATAGAGGGCTGCGTCACTTCTGGGGCC40.3972194637537239No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3972194637537239No Hit
GCTTTCAGAAGGCCCAGCAAGAATTTGCTGCTGGTGTCTTCTCCAACCCT40.3972194637537239No Hit
CCATATGATCAGGACAGCTTTTCCACTTTACTCGGTTTCCTACAAGCAAG40.3972194637537239No Hit
GGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGG40.3972194637537239No Hit
GTCAGGTGAAGGACTGTAAGCCCACACAAGCTCTTCTTATCTCTACTAGA30.29791459781529295No Hit
TTGGTGAACTATGAAATACGAAGGCCTTAATTTTGTACAATAAACTTTTA30.29791459781529295No Hit
CCTTTCAGTTGATATTTGCTTTTTGTGTTTATTTATATGTAACTAAGTCT30.29791459781529295No Hit
GGTATTCCCGATCTGCCATGTATTCCAGAAAGGCCATGTACAAGAGGAAG30.29791459781529295No Hit
GTTTTGGGTATTGTGGGTCTCTTACATTTTCAAATGGATTTTAGGATTAG30.29791459781529295No Hit
CTATAGCACCCCCTCTACCCCCTCTAAAACCAAAAAAAAAAAAAAAAAAA30.29791459781529295No Hit
GGGTAGAGAGGCACTTTCAGGGACTTCCCTCCAGCTGCCTCTTCATCTGG30.29791459781529295No Hit
GAACAAGACTCTCTCTCTCTCTCTCAGAATGATTCTGGCAAGGGCGACAG30.29791459781529295No Hit
TTCCCTACACGACGCGGGGGGTGAGACCAAAAAAAAAAAAAAAAAAAAAA30.29791459781529295No Hit
GTAGGCTCCCTTCCCCTACTCATCGCACTGATTTACACTCACAACACCCT30.29791459781529295No Hit
CCCTGGAAGTGGAGCCCAGTGACACCATCGAAAAAAAAAAAAAAAAAAAA30.29791459781529295No Hit
GGTCTAGAAAACCAGCTTGCGAATAACAGTCCCCGTGGCCATCCCTGTGA30.29791459781529295No Hit
GTATATAGGCTACTGAAAGAAGGATTCTGCATTTCTATTCCCCTCAGCCT30.29791459781529295No Hit
GCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTACT30.29791459781529295No Hit
GTAATAAATATGTAAGGCCTTTCTTTTTAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
ATATATGTGACAGATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
TTCCCTACACGACGCGGGGGGCACGGCCTCCGGCAGCTCCCGGAGCAAGA20.19860973187686196No Hit
GGAATTTCCCACCGCTTTGTGAGCCGTGTCGTATGACCTAGTAAACTTTG20.19860973187686196No Hit
GAGGGACCCTTGGCTCCTGTGTCTGGTCCACACACCACAGAAGCTTGTAT20.19860973187686196No Hit
ACATTGTGATACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
ATTATTCTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
GCGCCCAGTGGAGCCGTTTGGGAGAATTGCCTGCGCCACGCAGCGGGGCC20.19860973187686196No Hit
GAAATATGTGGATGAGGCTTCCAAGAAGGAGATCAAAGACATCCTCATCC20.19860973187686196No Hit
CCAACAGTGATGGTCAGCTAGATTTCTCAGAATTTCTTAATCTGATTGGT20.19860973187686196No Hit
TACATATGCTAAATAAACATAATTTAAAAAACAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
CCAAAAGCCAATAAAGTGATTACACCCGAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
AGCAAGTGCCGCCGGCCGGCTGTCAAGCAGTTCCACGACTCCAAGATCAA20.19860973187686196No Hit
GTATAATAAAGTATGGAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
GCTGTGCTCCAGCCTGGGTGACAAAGCGACTCCGTCTCTCAAAAAAAAAA20.19860973187686196No Hit
ATCCAGTGGTGGGTAATTGTACAATAAATTTTTTTTAATCAAAAAAAAAA20.19860973187686196No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAA20.19860973187686196No Hit
CACAAGTACTCCGGCAGGGAGGGTGACACGCACACCCTGAGCAAGAAGGA20.19860973187686196No Hit
GTGTAACGTTACATGACTCGTTGAGAAAGTTGAGGAATTTCCTCTACCAC20.19860973187686196No Hit
GGTCCAGGGCCCTGCGGGCCTCCCCCTGGCCATGGCCCAGGTCACCCACC20.19860973187686196No Hit
GCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTGCT20.19860973187686196No Hit
TTCCCTACACGACGCGGGGACATAAAGCCGGAAAATATCTTGATGTGTGT20.19860973187686196No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC20.19860973187686196No Hit
TGGCTTTCTCATGTAGGGCTCTCTGTGGTATTTGTTATTATTTTGCAACA20.19860973187686196No Hit
TCCCTACACGACGCGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
CCCTCTGGGGGTCCTCTCTGAGTCAAATCCAATAATAAATAATTATACAA20.19860973187686196No Hit
GGTCTCTCCCCCTTTGTTCAGCACTTCTTCCTGCCACCATGTGAAAAAAA20.19860973187686196No Hit
GTTTCATGCCCATCGTCCTAAAATTAATTCCCCTAAAAATCTTTAAAATA20.19860973187686196No Hit
AATGAGTTCATCTAATAAAATTGAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.19860973187686196No Hit
CTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTA20.19860973187686196No Hit
ATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAAT20.19860973187686196No Hit
TCCTCAGAGCTCAGGAGCGGGCTGCTGAGAGCTAAACCCAACAATTTTCT20.19860973187686196No Hit
CCCCCACTTCTTGAATAAAATAATTTAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
ACCTTCCATATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)