FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002877626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007
Sequences flagged as poor quality0
Sequence length71
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34834.55809334657398No Hit
CACAAGTACTCCGGCAGGGAGGGTGACAAGCACACCCTGAGCAAGAAGGAGCTGAAGGAGCTGATCCAGAA191.8867924528301887No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTACCCTATAG131.2909632571996028No Hit
AAATAATAATAATGCTATACTTAAATTACTTGTGAAAACAACACATTTTAAAGATTACGTGCTTCTTGGTA100.9930486593843099No Hit
GCTCTTCTGAGACTTCCATTCCTTTCTGATTGGCACACGTGCAGCTCATGACAATCTGTAGGATAACAATC90.893743793445879No Hit
GAATAAAAAACTCTCACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.7944389275074478No Hit
ACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCAGAGGTTGCGGTGAGCTGAGATTGCGCC80.7944389275074478No Hit
GCTTTAGAAAAGAAGTAAACTGGATACAAAAAGTTCCATTGAGGAACAGTTATTTACAGTATAAAAGATTT70.6951340615690168No Hit
GGTGTCAAGCAGTTCCACGACTCCAAGATCAAGTTCCCGGTGGGGCAGCGGGTCCTGCGCCGTCAGCACAA60.5958291956305859No Hit
GCTACTCGGGAGGCTGGGGCAAGAGAATCGCTTGAGCCTGGGAAGTAGAGGTTGGAGTGAGCCGAGATCGC60.5958291956305859No Hit
TAATAAACAGCCTCCTAGAGGCCACATTCTATTCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.5958291956305859No Hit
ATTATAGCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.49652432969215493No Hit
ATATATTCCTTAAAATATACATGGGGGCCTGAATGTCAGCCATCTTTATACTATAGAAAAAGGATTATGGA50.49652432969215493No Hit
TTGGCAGGCCAAGGAGGGTGGAAGACGAGGTCAAGAGATGAAGACCATCCTGGCTAACACAGTGAAACCCC50.49652432969215493No Hit
GTCCCAGCCTCGGGAGGGCACCTCCTTTTCTGTGCTTTCTTCCCTGAGGCATTCTTCCTCATCCCTAGGGT50.49652432969215493No Hit
CCACCCAGAGAAGCACACTTTGTGAGAACCAATGGGAAGGAGCCTGAGCTGCTGGAACCTATTCCCTATGA50.49652432969215493No Hit
CCTGCAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAAAATGGCTTGAACCTGGGAGGCAGAGGTTGCAGTG50.49652432969215493No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCT50.49652432969215493No Hit
CTGGATAATCAGTGTATGTTGCACCAGAATCAGCATTTTTTTTTTAACTGCAAAAAATGATGGTCTCATCT50.49652432969215493No Hit
GACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACA50.49652432969215493No Hit
CCATATGATCAGGACAGCTTTTCCACTTTACTCGGTTTCCTACAAGCAAGTAGGAAATACAGTGAATTTAC40.3972194637537239No Hit
CCCTGACTTTCTGAAGCTAGAAAAATGTTGTGTCTCCCAACCACCTTTCCATCCCCAGCCCCTCTCATCCC40.3972194637537239No Hit
GCTTTCAGAAGGCCCAGCAAGAATTTGCTGCTGGTGTCTTCTCCAACCCTGCGGTGCGAACCGCAGCTGCC40.3972194637537239No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3972194637537239No Hit
GTGGAAGGACCAATCACCTCTGATATTCTGTGGAAGGTTTTGGGGTCAAATTCTGCCCTCTGCATTCTGTG40.3972194637537239No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAAAAAAAAAAAAAAAAAAAAA40.3972194637537239No Hit
GTTTTGGGTATTGTGGGTCTCTTACATTTTCAAATGGATTTTAGGATTAGCTTGTCAATTTCTGCAAAAAA30.29791459781529295No Hit
GGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCC30.29791459781529295No Hit
GCGACTGAAGAAGATTCGGGCCCATAGAGGGCTGCGTCACTTCTGGGGCCTTCGTGTCCGAGGCCAGCACC30.29791459781529295No Hit
TTCCCTACACGACGCGGGGGGTGAGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29791459781529295No Hit
CCTTTCAGTTGATATTTGCTTTTTGTGTTTATTTATATGTAACTAAGTCTATTAATATAATAAAAATGAAT30.29791459781529295No Hit
GGTCTAGAAAACCAGCTTGCGAATAACAGTCCCCGTGGCCATCCCTGTGAGGGTGACGTTAGCATTACCCC30.29791459781529295No Hit
GGTATTCCCGATCTGCCATGTATTCCAGAAAGGCCATGTACAAGAGGAAGTACTCAGCCGCAAAAAAAAAA30.29791459781529295No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA30.29791459781529295No Hit
GCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCCTTATCTACTTCCTAATCCTATATACCCTTT30.29791459781529295No Hit
CTATAGCACCCCCTCTACCCCCTCTAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29791459781529295No Hit
TTGGCAGGCCAAGGAGGGTGGAAGACGAGGTCAAGAGATGAAGACCATCCTGGCTAACACAGTGAAACACC20.19860973187686196No Hit
GGGTAGAGAGGCACTTTCAGGGACTTCCCTCCAGCTGCCTCTTCATCTGGGAATGAGCTAAGCAAGGCTGC20.19860973187686196No Hit
TGGCTTTCTCATGTAGGGCTCTCTGTGGTATTTGTTATTATTTTGCAACAAGACCATTTTAGTAAAAAAAA20.19860973187686196No Hit
GTATATAGGCTACTGAAAGAAGGATTCTGCATTTCTATTCCCCTCAGCCTACCCACTAAAATCTTTAAATA20.19860973187686196No Hit
TACATATGCTAAATAAACATAATTTAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
GGTCTCTCCCCCTTTGTTCAGCACTTCTTCCTGCCACCATGTGAAAAAAAGATGTTTTTGCTTCCCCTTCC20.19860973187686196No Hit
CCCCCACTTCTTGAATAAAATAATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
CACAAGTACTCCGGCAGGGAGGGTGACACGCACACCCTGAGCAAGAAGGAGCTGAAGGAGCTGATCCAGAA20.19860973187686196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.19860973187686196No Hit
GCTCACTCACCCACCACATTAACAACATAAAACCCTCATTCACACGAGAAAACACCCTCATGTTCATACAC20.19860973187686196No Hit
CTGGATAATCAGTGTATGTTGCACCAGAATCAGCATTTTTTTTTTAACTGCAAAAAATGATGGTCTCATCA20.19860973187686196No Hit
GTATAATAAAGTATGGAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
ATTATTCTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
AGCAAGTGCCGCCGGCCGGCTGTCAAGCAGTTCCACGACTCCAAGATCAAGTTCCCGCTGCCCCACCGGGT20.19860973187686196No Hit
TTCCCTACACGACGCGGGGGGCACGGCCTCCGGCAGCTCCCGGAGCAAGAGCAAGGACCGCAAGTACACTC20.19860973187686196No Hit
GTAATAAATATGTAAGGCCTTTCTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
AATGAGTTCATCTAATAAAATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
CCAAAAGCCAATAAAGTGATTACACCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
TTGGTGAACTATGAAATACGAAGGCCTTAATTTTGTACAATAAACTTTTATTTATATTCTATATAAAAAAA20.19860973187686196No Hit
GAGCAAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAGAAAAAAAA20.19860973187686196No Hit
ACCTTCCATATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
GTTTCATGCCCATCGTCCTAAAATTAATTCCCCTAAAAATCTTTAAAATAAAACCCATATTTACCCTATAA20.19860973187686196No Hit
CCCTCTGGGGGTCCTCTCTGAGTCAAATCCAATAATAAATAATTATACAATAAATTTTTTTTAATCAAATT20.19860973187686196No Hit
TCCCTACACGACGCGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
GAGGGACCCTTGGCTCCTGTGTCTGGTCCACACACCACAGAAGCTTGTATTATCAGTGAATATAAATGTAC20.19860973187686196No Hit
GGAATTTCCCACCGCTTTGTGAGCCGTGTCGTATGACCTAGTAAACTTTGTACCAATTCAAAAAAAAAAAA20.19860973187686196No Hit
GAACAAGACTCTCTCTCTCTCTCTCAGAATGATTCTGGCAAGGGCGACAGAGTGAGAATCCGTCTCAAAAA20.19860973187686196No Hit
CACAAGTACTCCGGCAGGGAGGGTGACAAGCACACCCTGAGCAAGAAGGAGCTGAAGGAGCTGCTCCAGAA20.19860973187686196No Hit
GCGCCCAGTGGAGCCGTTTGGGAGAATTGCCTGCGCCACGCAGCGGGGCCGGACAGGCCGCACAGACCTAC20.19860973187686196No Hit
ACATTGTGATACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
CCAACAGTGATGGTCAGCTAGATTTCTCAGAATTTCTTAATCTGATTGGTGGCCTAGCTATGGCTTGCCAT20.19860973187686196No Hit
ATCCAGTGGTGGGTAATTGTACAATAAATTTTTTTTAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)