FastQCFastQC Report
Wed 8 May 2024
EGAF00002877630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002877630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832748
Total Bases59.1 Mbp
Sequences flagged as poor quality0
Sequence length71
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126051.5136631970295937No Hit
GTTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG34950.41969479362304085Illumina Single End PCR Primer 1 (100% over 45bp)
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA23850.28640116818053No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18750.22515815108532233No Hit
ATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCATCGTCCTAG16150.19393622080149095No Hit
GGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCA14360.17244112264454553No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC10520.1263287333022715No Hit
GTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCAT8740.1049537194925716No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)